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Special Issue "Technical Advances in Light Microscopy"
A special issue of Methods and Protocols (ISSN 2409-9279).
Deadline for manuscript submissions: closed (15 May 2019).
Dr. Pablo Loza-Alvarez Website E-Mail
ICFO-Institut de Ciencies Fotoniques, The Barcelona Institute of Science and Technology Mediterranean Technology Park, Av. Carl Friedrich Gauss, 3 08860 Castelldefels (Barcelona), Spain
Interests: light sheet microscopy; super-resolution microscopy; multiphoton microscopy
The different imaging modalities, including confocal, multiphoton, light sheet, and super-resolution, etc., can be collectively called advanced light microscopy technologies. Each technique has specific advantages and drawbacks that appeal to different researchers, depending on their specific applications and needs. In fact, despite the latest efforts, biomedical researchers are still looking for novel ways for visualising organisms, tissues, cells and subcellular components. In each case, higher resolutions, larger fields of view and penetration depths, faster imaging speeds, higher signal to noise ratios and better sensitivities, etc. keep to be sought. On top of that, all of these are to be compatible with in vivo imaging. For example, multiscale imaging (from a few nm to mm) in neurobiology would allow observation of how far synapses are interconnected in the brain. Fast dynamic observations of processes in the whole sample could be used for a precise tracking of dividing cells during long development periods. Light-based manipulation of the samples, including optogenetics and photoactivation could enable the unravelling of protein transport during transduction of light into signals on retinas, and so on. To address these issues, a multi-disciplinary cutting-edge research effort is necessary. This should combine different scientific disciplines such as optics and photonics, engineering, medicine, chemistry and mathematics. In this Special Issue on “Technical Advances in Light Microscopy”, we welcome original research and review articles dealing with novel technical implementations in light microscopy used to tackle important bio-medical questions. Approaches include the implementation of multimodal microscopy imaging, the use of novel scientific instrumentation, the use of novel fluorescent markers, the development of modelling and algorithms for image quantification and analysis and so on.
Dr. Pablo Loza-Alvarez
Manuscript Submission Information
Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All papers will be peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.
Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Methods and Protocols is an international peer-reviewed open access quarterly journal published by MDPI.
Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 1000 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.
- Light sheet microscopy (SPIM, DSLM, DiSPIM, etc.)
- Nonlinear microscopy (TPEF, SHG, THG, etc.)
- Super resolution microscopy (STED, STORM, PALM, structured illumination etc.)
- Lattice light sheet microscopy
- Photoexcitation and photoablation
- Optical tweezers
- Adaptive Optics
- Wavefront shaping
- Beam engineering
- Nonlinear and specialised dyes
- Deconvolution algorithms
- Inverse problem solving
- Hyperspectral imaging
- Machine learning