Next Article in Journal / Special Issue
Enhanced Light Sheet Elastic Scattering Microscopy by Using a Supercontinuum Laser
Previous Article in Journal
Recovery and Detection of Enteric Viruses from Non-Traditional Irrigation Water Sources
Previous Article in Special Issue
The DIVER Microscope for Imaging in Scattering Media
Open AccessProtocol

Protocol for the Design and Assembly of a Light Sheet Light Field Microscope

1
Biomedical Computer Vision Group, Universidad de los Andes, Bogota 111711, Colombia
2
Biophysics Laboratory, Universidad de los Andes, Bogota 111711, Colombia
*
Author to whom correspondence should be addressed.
Methods Protoc. 2019, 2(3), 56; https://doi.org/10.3390/mps2030056
Received: 16 May 2019 / Revised: 29 June 2019 / Accepted: 1 July 2019 / Published: 4 July 2019
(This article belongs to the Special Issue Technical Advances in Light Microscopy)
Light field microscopy is a recent development that makes it possible to obtain images of volumes with a single camera exposure, enabling studies of fast processes such as neural activity in zebrafish brains at high temporal resolution, at the expense of spatial resolution. Light sheet microscopy is also a recent method that reduces illumination intensity while increasing the signal-to-noise ratio with respect to confocal microscopes. While faster and gentler to samples than confocals for a similar resolution, light sheet microscopy is still slower than light field microscopy since it must collect volume slices sequentially. Nonetheless, the combination of the two methods, i.e., light field microscopes that have light sheet illumination, can help to improve the signal-to-noise ratio of light field microscopes and potentially improve their resolution. Building these microscopes requires much expertise, and the resources for doing so are limited. Here, we present a protocol to build a light field microscope with light sheet illumination. This protocol is also useful to build a light sheet microscope. View Full-Text
Keywords: light field microscopy; light sheet fluorescence microscopy; LSFM; 3D imaging; fast imaging; open science light field microscopy; light sheet fluorescence microscopy; LSFM; 3D imaging; fast imaging; open science
Show Figures

Figure 1

MDPI and ACS Style

Madrid-Wolff, J.; Forero-Shelton, M. Protocol for the Design and Assembly of a Light Sheet Light Field Microscope. Methods Protoc. 2019, 2, 56.

Show more citation formats Show less citations formats
Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Article Access Map

1
Back to TopTop