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Recent Advances in Mass Spectrometry Analysis for Medical and Pharmaceutical...
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Recent Advances in Mass Spectrometry Analysis for Medical and Pharmaceutical Applications

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: closed (31 March 2025) | Viewed by 2476

Special Issue Editor

Prof. Dr. Wojciech Piekoszewski

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Faculty of Chemistry, Jagiellonian University, Krakow, Poland
Interests: proteomics; analysis of biologically active compounds; LC-MS/MS; pharmacokinetics; lipidomics; biomarkers
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Recent mass spectrometry (MS) has significantly impacted medical and pharmaceutical applications, enabling more precise and sensitive analyses. Mass spectrometry has evolved from a tool primarily used for molecular weight determination to a sophisticated technique capable of detailed structural analysis and quantification of complex biomolecules. These innovations have accelerated the development of diagnostic biomarkers, therapeutic drug monitoring, and the study of drug metabolism. Furthermore, recent improvements in instrumentation, such as high-resolution MS and tandem mass spectrometry, have enhanced the detection of small molecules, peptides, proteins, and metabolites in biological samples, contributing to personalized medicine and pharmacokinetics. Advances in sample preparation techniques, data analysis algorithms, and bioinformatics integration have also expanded the scope of MS applications in proteomics, metabolomics, and lipidomics. The use of mass spectrometry in clinical settings and pharmaceutical development continues to grow, offering improved accuracy and efficiency in drug discovery, toxicology, and disease monitoring. This Special Issue covers recent developments and future trends in MS-based approaches for medical and pharmaceutical research.

Prof. Dr. Wojciech Piekoszewski
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • mass spectrometry (MS)
  • biomarkers
  • drug metabolism
  • proteomics
  • metabolomics
  • pharmaceutical analysis
  • personalized medicine
  • therapeutic drug monitoring

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Published Papers (3 papers)

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Research

22 pages, 6822 KiB  
Article
The Proteomic Analysis of Platelet Extracellular Vesicles in Diabetic Patients by nanoLC-MALDI-MS/MS and nanoLC-TIMS-MS/MS
by Joanna Kasprzyk-Pochopień, Agnieszka Kamińska, Przemysław Mielczarek, Radosław Porada, Ewa Stępień and Wojciech Piekoszewski
Molecules 2025, 30(6), 1384; https://doi.org/10.3390/molecules30061384 - 20 Mar 2025
Viewed by 460
Abstract
Platelet extracellular vesicles (PEVs) are emerging as key biomarkers in diabetes mellitus (DM), reflecting altered platelet function and coagulation pathways. This study compares two proteomic techniques—nanoLC-MALDI-MS/MS and nanoLC-TIMS-MS/MS—for analyzing PEVs in diabetic patients, to assess their potential for biomarker discovery. PEVs were isolated [...] Read more.
Platelet extracellular vesicles (PEVs) are emerging as key biomarkers in diabetes mellitus (DM), reflecting altered platelet function and coagulation pathways. This study compares two proteomic techniques—nanoLC-MALDI-MS/MS and nanoLC-TIMS-MS/MS—for analyzing PEVs in diabetic patients, to assess their potential for biomarker discovery. PEVs were isolated from platelet-rich plasma and characterized using tunable resistive pulse sensing (TRPS), Fourier-transform infrared (FTIR) spectroscopy, and transmission electron microscopy (TEM). Proteomic analyses identified significant differences in protein expression between diabetic and non-diabetic individuals, with nanoLC-TIMS-MS/MS demonstrating superior sensitivity by detecting 97% more unique proteins than nanoLC-MALDI-MS/MS. Key differentially expressed proteins included apolipoproteins and oxidative stress markers, which may contribute to platelet dysfunction and cardiovascular complications in DM. Sex-specific variations in protein expression were also observed, highlighting potential differences in disease progression between male and female patients. The integration of advanced proteomic methodologies provides novel insights into the role of PEVs in diabetes pathophysiology, underscoring their diagnostic and therapeutic potential. These findings pave the way for improved biomarker-based strategies for early detection and monitoring of diabetic complications. Full article
(This article belongs to the Special Issue Recent Advances in Mass Spectrometry Analysis for Medical and Pharmaceutical Applications)
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18 pages, 5351 KiB  
Article
Characterization of Honey Microbiome Using MALDI-TOF Mass Spectrometry and Physicochemical Study
by Dominika Błońska and Bogusław Buszewski
Molecules 2025, 30(6), 1266; https://doi.org/10.3390/molecules30061266 - 12 Mar 2025
Viewed by 525
Abstract
Honey, a super-saturated solution produced by Apis mellifera, is well-known for its historical medicinal uses, as well as culinary applications. Comprising sugars, phenols, enzymes, and more, its complex composition contributes to its medicinal properties. The microbiome, dominated by spore-forming bacteria and yeasts, [...] Read more.
Honey, a super-saturated solution produced by Apis mellifera, is well-known for its historical medicinal uses, as well as culinary applications. Comprising sugars, phenols, enzymes, and more, its complex composition contributes to its medicinal properties. The microbiome, dominated by spore-forming bacteria and yeasts, is also a crucial factor in the health benefit properties of honey. The identification of the microbiome of honeys contributes to a better understanding of their microbial landscape and health-benefit properties and is also relevant to the environmental aspect. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) is emerging as a key tool for microbial identification, but challenges remain in ensuring accuracy under different conditions. This study focuses on developing optimal conditions for microbial isolation and culture, aiming to balance diversity and avoid negative effects on identification. It further has the objective of evaluating the influence of geographic and botanical factors on the composition and diversity of the honey microbiome. Full article
(This article belongs to the Special Issue Recent Advances in Mass Spectrometry Analysis for Medical and Pharmaceutical Applications)
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27 pages, 6354 KiB  
Article
Potential Clinical Application of Analysis of Bisphenols in Pericardial Fluid from Patients with Coronary Artery Disease with the Use of Liquid Chromatography Combined with Fluorescence Detection and Triple Quadrupole Mass Spectrometry
by Tomasz Tuzimski, Szymon Szubartowski, Janusz Stążka, Kamil Baczewski, Daria Janiszewska, Viorica Railean, Bogusław Buszewski and Małgorzata Szultka-Młyńska
Molecules 2025, 30(1), 140; https://doi.org/10.3390/molecules30010140 - 1 Jan 2025
Cited by 1 | Viewed by 1017
Abstract
Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, [...] Read more.
Bisphenols may negatively impact human health. In this study, we propose the use of HPLC–FLD for the simultaneous determination of bisphenols in pericardial fluid samples collected from patients with coronary artery disease undergoing coronary artery bypass surgery. For sample preparation, a fast, simple, and ”green” DLLME method was used, achieving mean recovery values in the range of 62%–98% with relative standard deviations between 2% and 6% for all analytes. Quantitative analysis of bisphenols in the samples was then performed by LC–MS/MS on a triple quadrupole (QqQ) mass spectrometer and electrospray ionization (ESI-/ESI+) was applied in the negative and positive ion modes, respectively. The LODs and LOQs ranged from 0.04 ng/mL to 0.37 ng/mL and 0.12 ng/mL to 1.11 ng/mL, respectively. Pericardial fluid was collected from patients with coronary artery disease during coronary artery bypass surgery. Bisphenol residues were identified and quantified in samples from 19 patients. The procedure was successfully applied to the biomonitoring of free forms of 14 bisphenols in pericardial fluid. After statistical examination of the relationships between the selected variables, a strongly positive correlation was found between creatinine kinase and troponin I, as well as the number of venous anastomoses, circulation time, and clamp cap time. Full article
(This article belongs to the Special Issue Recent Advances in Mass Spectrometry Analysis for Medical and Pharmaceutical Applications)
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