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The Application of LC-MS in Pharmaceutical Analysis—2nd Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 30 January 2026 | Viewed by 300

Special Issue Editors


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Guest Editor
Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, SHM B254, New Haven, CT 06510, USA
Interests: drug-drug interaction analysis; cell signaling; herbal chemcials
Special Issues, Collections and Topics in MDPI journals
Deputy Dean of School of Pharmaceutical Sciences and Director of Pharmaceutical Analysis Teaching and Research Office, Hunan University of Medicine, Huaihua 41800, China
Interests: LC-MS; metabolites; pharmaceutical analysis
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

In recent decades, liquid chromatography–mass spectrometry (LC-MS) and ultra-high-performance liquid chromatography combined with high-resolution mass spectrometry (UHPLC-HRMS) have gained significant prominence as vital analytical instruments, particularly the latter. Their superior separating efficiency, specificity, and sensitivity have rendered them indispensable in various fields, especially in pharmaceutical analysis. Therefore, we propose an in-depth exploration of the applications and advancements of LC-MS in pharmaceutical analysis through a dedicated Special Issue, titled “The application of LC-MS in pharmaceutical analysis”.

This Special Issue aims at gathering and disseminating the latest research, methodologies, and findings related to the application of LC-MS in pharmaceutical analysis. We envision a comprehensive collection of manuscripts that cover a wide range of themes, including (but not limited to) the following:

  1. Method development and validation using LC-MS for pharmaceutical analysis;
  2. Chemical characterization and metabolite identification using LC-MS;
  3. Untargeted and targeted metabolomics studies employing LC-MS;
  4. Pharmacokinetic analysis using LC-MS techniques;
  5. High-throughput LC-MS approaches for drug discovery and analysis;
  6. The analysis and identification of impurities and degradation products using LC-MS.

We believe that this Special Issue will serve as an excellent platform for researchers and practitioners in the field to share their valuable insights, novel methodologies, and significant findings. It will not only contribute to the existing knowledge base but also stimulate further advancements in LC-MS technology and its applications in pharmaceutical analysis.

Dr. Wing Lam
Dr. Wei Cai
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Molecules is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2700 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • LC–MS
  • chemical characterization
  • metabolite identification
  • metabolomics
  • pharmacokinetics

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Published Papers (1 paper)

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Research

19 pages, 1078 KiB  
Article
Antioxidant Activity and Phytochemical Profiling of Steam-Distilled Oil of Flaxseed (Linum usitatissimum): Therapeutic Targeting Against Glaucoma, Oxidative Stress, Cholinergic Imbalance, and Diabetes
by İlhami Gulcin, Muzaffer Mutlu, Zeynebe Bingol, Eda Mehtap Ozden, Ziba Mirzaee, Ahmet C. Goren and Ekrem Köksal
Molecules 2025, 30(16), 3384; https://doi.org/10.3390/molecules30163384 - 14 Aug 2025
Abstract
This investigation explored the chemical constituents and biological activities of the steam-distilled oil of L. usitatissimum (SDOLU), employing sophisticated techniques including LC-HRMS, GC-MS, and GC-FID. The analysis identified a diverse array of 17 phenolic compounds, with linoleoyl chloride (64.05%) and linoleic acid (10.39%) [...] Read more.
This investigation explored the chemical constituents and biological activities of the steam-distilled oil of L. usitatissimum (SDOLU), employing sophisticated techniques including LC-HRMS, GC-MS, and GC-FID. The analysis identified a diverse array of 17 phenolic compounds, with linoleoyl chloride (64.05%) and linoleic acid (10.39%) as the major fatty acid components. The SDOLU demonstrated remarkable antioxidant capacity, effectively neutralizing free radicals in both DPPH (IC50: 19.80 μg/mL) and ABTS•+ (IC50: 57.75 μg/mL) scavenging assays, alongside robust electron-donating activity in reducing ability tests. Moreover, the SDOLU showed significant inhibition of key enzymes implicated in metabolic and neurodegenerative disorders, including α-amylase (IC50: 531.44 μg/mL), acetylcholinesterase (IC50: 13.23 μg/mL), and carbonic anhydrase II (IC50: 281.02 μg/mL). Collectively, these results highlight the SDOLU as a valuable natural source of multifunctional bioactivities with potential applications in combating oxidative stress and enzyme-related global diseases. Further studies are warranted to validate its therapeutic efficacy and expand its industrial utilization. Full article
(This article belongs to the Special Issue The Application of LC-MS in Pharmaceutical Analysis—2nd Edition)
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