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A New Diagnosis Tool of Cancer by Spectroscopic Analysis-Second Edition

A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Analytical Chemistry".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 845

Special Issue Editors


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Guest Editor
Department of Molecular Chemistry, National University of Kyoto Institute of Technology, Kyoto, Japan
Interests: FT-IR microscopy; cancer imaging; fluorescence spectroscopy; far infrared spectroscopy
Special Issues, Collections and Topics in MDPI journals

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Guest Editor
Quantitative RedOx Sensing Group, Department of Radiation Regulatory Science Research, Institute for Radiological Science (NIRS), National Institutes for Quantum Science and Technology (QST), 4-9-1 Anagawa, Inage-ku, Chiba 263-8555, Japan
Interests: electron paramagnetic resonance; electron spin resonance; free radical; reactive oxygen species; oxidative stress; magnetic resonance imaging; spectral-spatial imaging; redox imaging; oxygen; oxymetry; oxygen mapping; radiation chemistry; particle beam; heavy-ion beam

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Guest Editor
Graduate School of Biological and Environmental Sciences, Kwansei Gakuin University, 2-1 Gakuen, Sanda 669-1337, Japan
Interests: cancer; FTIR; immunohistochemistry; Raman spectroscopy; TEM

Special Issue Information

Dear Colleagues,

Diagnosis of human cancer by pathologists has been based on the hematoxylin–eosin (HE) stain for the last 140 years. Recently, however, many new kinds of spectroscopic analysis have suddenly been developed, especially in the field of the infrared region with laser and algorithm data analysis technologies, similar to a type of nuclear magnetic resonance imaging (MRI). Conventional staining is performed using innovative tools such as MRI, which can quantitatively visualize biological components in fresh tissue (frozen sections) and visualize seismic components in the invisible infrared region. We have planned the second edition of this Special Issue in order to break the jinx that a diagnosis cannot be made unless a diagnosis is made.

Spectroscopic analysis technology does not involve stain treatment of the slice of cancer tissue, especially raw tissue by Raman, Fourier transform infrared (FT-IR), and mass spectroscopic microscopes. In the raw tissue sample, no artificial image information on lipids, protein secondary conformation, or water molecules in cancerous patients is missed with HE, as with MRI.

This Special Issue will identify the spectroscopic parameters dependent on cancer stages, malignancies, and how to respond to the many kinds of differentiations of the morphological parameters for each cancer case in the future with the help of analysis techniques of an algorithm, chemometric analysis, and a fractal theory to solve image correlation and/or similarity of HE stains.

Prof. Dr. Norio Miyoshi
Dr. Ken-ichiro Matsumoto
Dr. Bibin B. Andriana
Guest Editors

Manuscript Submission Information

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Keywords

  • FT-IR microscope
  • FT-IR markers of cancer
  • mapping images
  • HE stained
  • images
  • water molecule distribution
  • T2-MRI

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Published Papers (1 paper)

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Research

15 pages, 1171 KiB  
Article
An Electron Paramagnetic Resonance Study of the Superoxide-Scavenging and Redox-Modulating Effects of Lecithinized Superoxide Dismutase in the Bloodstream
by Dessislava Lazarova, Plamen Getsov, Rumiana Bakalova, Biliana Nikolova, Severina Semkova, Zhivko Zhelev, Zhiwei Qiao, Tomohiro Ishikawa, Koichiro Fukuda, Kensuke Osada, Milka Mileva, Tohru Mizushima and Ichio Aoki
Molecules 2025, 30(9), 1882; https://doi.org/10.3390/molecules30091882 - 23 Apr 2025
Viewed by 164
Abstract
Lecithinized superoxide dismutase (PC-SOD) was found to have a significantly improved half-life in the bloodstream and better pharmacological effects compared with unmodified SOD. However, there is no direct evidence that parenterally administered PC-SOD decreases superoxide levels in blood and tissues in vivo. In [...] Read more.
Lecithinized superoxide dismutase (PC-SOD) was found to have a significantly improved half-life in the bloodstream and better pharmacological effects compared with unmodified SOD. However, there is no direct evidence that parenterally administered PC-SOD decreases superoxide levels in blood and tissues in vivo. In the present study, we investigated the ability of PC-SOD versus unmodified SOD as a superoxide scavenger in mice subjected to oxidative stress. Experiments were performed on a lipopolysaccharide (LPS) mouse model of acute inflammation known to be accompanied by the overproduction of superoxide in the blood. The mice were divided into four groups: untreated (healthy; n = 6), LPS-treated (n = 7), LPS/SOD-treated (n = 6), and LPS/PC-SOD-treated (n = 7) mice. SOD and PC-SOD were injected intravenously. Blood samples were collected at four time intervals and analyzed by electron paramagnetic resonance (EPR) spectroscopy using a nitroxide probe, 3-carbamoyl-2,2,5,5-tetramethylpyrrolidine-1-oxyl (CMP). The following effects were observed: (i) In the blood of healthy mice, the EPR signal was significantly lower compared with the control (p < 0.001) and LPS-treated mice (p < 0.01); (ii) in the blood of LPS-treated mice, the EPR signal was identical to that of the control; and (iii) in the blood of LPS/SOD-treated mice collected immediately after enzyme injection, the EPR signal was significantly lower compared with the control (p < 0.01) and LPS-treated mice (p < 0.05). However, the effect disappeared in the samples collected 30 min and 1 h after enzyme injection. (iv) In LPS/PC-SOD-treated mice, the EPR signal was significantly lower compared with the control (p < 0.01) and LPS-treated mice (p < 0.05), even in the blood samples collected within 1 h after enzyme injection. The data indicate that the blood of healthy mice was characterized by a high reducing capacity, while the blood of LPS-treated mice was characterized by a high oxidative capacity. SOD decreased superoxide production immediately after enzyme injection. However, the effect was short-lived and disappeared within 30 min. PC-SOD effectively decreased superoxide production in the bloodstream of LPS-treated mice and restored the redox balance to the control level even two hours after enzyme injection. The effects of PC-SOD were more pronounced and long-lasting compared with those of SOD. The possible reason is the longer half-life of PC-SOD in the bloodstream, its better stability, and its slower clearance from the circulation due to the increased hydrophobicity of the enzyme and its interaction with plasma proteins. The data are discussed in the context of recent clinical trials showing that PC-SOD is a promising pharmaceutical product for adjuvant therapy of a variety of pathologies accompanied by inflammation, redox imbalance, and oxidative stress. Full article
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