Advancements in Enzyme Immobilization Techniques
A special issue of Molecules (ISSN 1420-3049). This special issue belongs to the section "Applied Chemistry".
Deadline for manuscript submissions: 30 April 2026 | Viewed by 6
Special Issue Editors
Interests: protein engineering; chimeric proteins applications; protein production; modification of active centers for different industrial applications; proteins for hydrocarbon biosulfurization; synthetic proteins; design of matrices for protein purification; proteins and different industrial applications; multienzyme proteins
Special Issues, Collections and Topics in MDPI journals
Interests: enzyme engineering; enzymatic biotransformation engineering; enzymatic food biotechnology
Special Issues, Collections and Topics in MDPI journals
Special Issue Information
Dear Colleagues,
Enzyme immobilization has progressed significantly in recent years, improving enzyme stability, activity, and reusability for industrial and biomedical applications. In this special issue, we would like to focus on topics related to enzyme immobilization, which allows for the application and use of cutting-edge, state-of-the-art immobilization techniques.
Support Materials: Development of novel carriers such as nanomaterials (magnetic nanoparticles, carbon nanotubes, MOFs, graphene), biopolymers, and hybrid organic–inorganic matrices has expanded immobilization options.
Immobilization Methods: Beyond traditional adsorption, covalent binding, entrapment, and cross-linking, new strategies like site-directed immobilization, click chemistry, and genetic fusion tags (e.g., His-tag, SpyTag/SpyCatcher systems) enhance orientation and activity.
Multifunctional Supports: Smart and stimuli-responsive supports allow controlled enzyme release or activation under specific conditions (pH, temperature, light).
Co-immobilization: Immobilizing multiple enzymes in cascade reactions has enabled efficient biotransformations, mimicking metabolic pathways.
Stabilization: Advances in cross-linked enzyme aggregates (CLEAs), nanogel entrapment, and multipoint covalent attachment have greatly improved resistance to denaturation, solvents, and high temperatures.
Applications: Modern immobilization techniques drive progress in biocatalysis, biosensors, bioremediation, drug delivery, and food technology.
Overall, advancements focusing on enhancing enzyme performance, enabling process intensification, and expanding applications in sustainable biotechnology are proposed for this Special Issue.
Dr. Benevides C. Pessela
Dr. Gloria Fernandez-Lorente
Guest Editors
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Keywords
- enzyme immobilization
- biocatalysis
- enzyme stabilization
- reusability/recyclability
- activity retention
- operational stability
- mass transfer limitation
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