Diagnosis, Treatment, and Prevention of Biofilm Infections—How to Proceed?

A special issue of Microorganisms (ISSN 2076-2607). This special issue belongs to the section "Biofilm".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 2122

Editors


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Guest Editor
1. Institute of Immunology and Microbiology, Costerton Biofilm Center, Faculty of Health Science, University of Copenhagen, DK-2200 Copenhagen, Denmark
2. Department of Clinical Microbiology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, Denmark
Interests: biofilms; chronic infection; P. aeruginosa; antibiotic resistance mechanisms

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Co-Guest Editor
1. Institute of Immunology and Microbiology, Costerton Biofilm Center, Faculty of Health Science, University of Copenhagen, DK-2200 Copenhagen, Denmark
2. Department of Clinical Microbiology, Rigshospitalet, University of Copenhagen, DK-2100 Copenhagen, Denmark
Interests: biofilm; microbiology; Pseudomonas aeruginosa; bacteria and immunology

Special Issue Information

Dear Colleagues,

The ESCMID guidelines of diagnosis and treatment of biofilm infections were published in 2015 [1] and the need for new methods and research was discussed in 2023 [2]. The purpose of this Special Issue is, therefore, to invite biofilm scientists to publish original articles or survey articles regarding diagnosis, treatment, and prevention of medical biofilm infections. Such articles may include in vitro experiments or animal experiments with clear clinical possibilities of reaching clinical fase 2 trials, or the articles that show promising or convincing in vivo clinical results of new diagnostic, therapeutic, or prophylactic methods, which can then be investigated in larger clinical trials. The submitted methods and articles should emphasize how planktonic and biofilm infections can be reliably differentiated by the new methods, which can be used for early biofilm infections (infections lasting days or weeks) and/or ‘old’ biofilm infections (old infections lasting months or years).

We look forward to your submissions.

  1. N. Høiby, T. Bjarnsholt, C. Moser, GL Bassi, T. Coenye, G. Donelli, L. Hall-Stoodley, V. Holá, C. Imbert, K. Kirketerp-Møller, D. Lebeaux, A. Oliver, A. J. Ullmann and C. Williams for the ESCMID Study Group for Biofilms (ESGB) and consulting external expert Werner Zimmerli.: ESCMID* guideline for the diagnosis and treatment of biofilm infections 2014. Clin. Microbiol Infect. 21, Supplementum 1: 1-26; 2015.
  2. Høiby, N., Moser, C., Oliver, A., Williams, C., Ramage, G., Borghi, E., Azeredo, J., Macia, M.D. for the ESGB.: Time to update the ESCMID Guidelines for the diagnosis and treatment of biofilm infections? Biofilm, June 2023. doi.org./10.1016/j.biofilm.2023100135.

Prof. Dr. Niels Høiby
Prof. Dr. Claus Moser
Guest Editors

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Keywords

  • biofilm
  • biofilm infections
  • chronic infections
  • foreign body infections
  • implant infections

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Published Papers (1 paper)

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Research

16 pages, 1338 KB  
Article
Biofilm Formation in Aspergillus fumigatus: A Comparative Study of Strains from Different Origins
by Marta Cano-Pérez, Juan de Dios Caballero Pérez, Elia Gómez García de la Pedrosa and Alicia Gómez-López
Microorganisms 2026, 14(2), 272; https://doi.org/10.3390/microorganisms14020272 - 24 Jan 2026
Cited by 1 | Viewed by 1576
Abstract
One of the most notable aspects of Aspergillus fumigatus, and related to its dynamic adaptation, is its ability to form biofilm and produce a wide variety of secondary metabolites. The aim of this study is to advance the characterization of biofilms generated by [...] Read more.
One of the most notable aspects of Aspergillus fumigatus, and related to its dynamic adaptation, is its ability to form biofilm and produce a wide variety of secondary metabolites. The aim of this study is to advance the characterization of biofilms generated by different A. fumigatus strains across their developmental stages and analytically evaluate their structure and composition and their relationship with secondary metabolism activation. An in vitro biofilm model was standardized to investigate structural and analytical differences among strains isolated from distinct clinical settings and associated with different pathologies. We found that all tested strains could form biofilms; however, the characteristics of these structures—including total biomass, cellular viability and overall structure—varied markedly among strains under the evaluated conditions. Strains isolated from cystic fibrosis patients exhibited distinct behaviors in most conducted assays compared to other strains. These findings provide new insights into the variability of biofilm composition and may contribute to a better understanding of the role of biofilms in fungal pathogenesis, persistence and treatment resistance. Full article
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