Research and Application of Fungal Enzymes

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungi in Agriculture and Biotechnology".

Deadline for manuscript submissions: 30 November 2026 | Viewed by 671

Special Issue Editor


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Guest Editor
Department of Food Science and Biotechnology, Faculty of Chemistry, National Autonomous University of Mexico, Coyoacan, Mexico
Interests: Aspergillus; fungal enzymes; filamentous fungi

Special Issue Information

Dear Colleagues,

Filamentous fungi are important organisms in human life. The production and application of enzymes is one of the areas where these microorganisms have a broad impact. Among the other important characteristics of filamentous fungi is their secretion capacity: they produce countless extracellular enzymes with a wide range of applications.

They have applications in the food industry, bioenergy and environmental sustainability, the textile industry, and detergents. Also of great importance are their applications in the pulp and paper, pharmaceutical, and healthcare industries.

The future of fungal enzymes is promising and is closely linked to biotechnology. The accelerated development of omics sciences and the increasingly widespread use of artificial intelligence, together with enzyme engineering, molecular biology, and other scientific disciplines, will make it possible to obtain enzymes with improved characteristics. This will also enhance the capacity for the search, identification, and characterization of new fungal strains and enzymes with unique biocatalytic activities for new applications in green chemistry and medicine. Thermostable enzymes, new microorganisms, and new applications can be found because of multidisciplinary research in various fields related to fungi and enzymes.

We are pleased to invite you to contribute to this Special Issue, ‘Research and Application of Fungal Enzymes’. Submissions of original research articles, reviews, methods, and communications are welcome. Research areas may include, but are not limited to, the applications of proteomics, metabolomics, genomics, transcriptomics, biochemical engineering, fermentation technology, and crispr-cas9 to the production and regulation of fungal enzymes. Enzyme immobilization and new approaches to enzyme production, either by submerged or solid-state fermentation, will also be included. New applications in food and feed, medicine, and healthcare, are also welcome.

The overall objective of this Special Issue is to provide a sample of the state of the art in the research and applications of fungal enzymes.

Dr. Guillermo Aguilar Osorio
Guest Editor

Manuscript Submission Information

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Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fungal enzymes
  • filamentous fungi
  • enzyme
  • production extracellular
  • enzymes enzyme
  • application biotechnology
  • enzyme engineering

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Published Papers (1 paper)

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Research

16 pages, 3570 KB  
Article
Engineering a Cold-Active Cellulase Complex with a Novel Mushroom Cellobiohydrolase for Efficient Biomass Saccharification and Juice Flavor Optimization
by Jiaqi Yang, Youran Shao, Ying Wang, Ming Gong, Bing Li, Hongyu Chen, Caizhen Wang, Yan Li, Xiang Zhou and Gen Zou
J. Fungi 2026, 12(4), 276; https://doi.org/10.3390/jof12040276 - 10 Apr 2026
Viewed by 457
Abstract
Cold-active cellulases are highly desirable for temperature-sensitive biomass valorization and food processing, yet they remain scarce in conventional industrial fungal platforms. In this study, a novel cold-induced cellobiohydrolase, VvCBHI-II, was mined from the mushroom Volvariella volvacea and successfully engineered into the industrial [...] Read more.
Cold-active cellulases are highly desirable for temperature-sensitive biomass valorization and food processing, yet they remain scarce in conventional industrial fungal platforms. In this study, a novel cold-induced cellobiohydrolase, VvCBHI-II, was mined from the mushroom Volvariella volvacea and successfully engineered into the industrial workhorse Trichoderma reesei via site-specific homologous replacement. Structural homology modeling revealed that the substitution of the flexible B3 loop with a β-sheet creates a more open substrate-binding cleft in VvCBHI-II. Consequently, the purified VvCBHI-II exhibited robust endoglucanase-like characteristics with superior catalytic efficiency on amorphous cellulose. At 10 °C, the engineered cellulase complex demonstrated an 8.1-fold increase in filter paper activity compared to the wild-type strain. Mechanistic structural analyses indicated that the open cleft architecture elongates and weakens the hydrogen-bonding network with the cellobiose product, facilitating rapid product dissociation and alleviating severe cold-induced product inhibition. In practical applications, the engineered cold-active enzyme complex exhibited an exceptional saccharification capacity on natural pear pomace at 10 °C. Furthermore, when applied to simulated fruit juice processing, it significantly maximized the extraction yield, elevated the sweetness response, and substantially mitigated undesirable bitterness and astringency. This study elucidates the structural-functional paradigm of cold-adapted cellobiohydrolases and provides a promising strategy for formulating highly efficient, energy-saving biocatalysts for the food and biorefinery industries. Full article
(This article belongs to the Special Issue Research and Application of Fungal Enzymes)
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