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Biotechnological Applications of Fungi
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Biotechnological Applications of Fungi

A special issue of Journal of Fungi (ISSN 2309-608X). This special issue belongs to the section "Fungi in Agriculture and Biotechnology".

Deadline for manuscript submissions: 15 May 2026 | Viewed by 1690

Special Issue Editors

Prof. Dr. Luis Henrique Ferreira Do Vale

E-Mail Website
Guest Editor
Department of Cell Biology, Institute of Biology, University of Brasília (UnB), Brasília, Brazil
Interests: fungal biotechnology; proteomics; bioremediation; enzyme discovery; environmental microbiology; enzymology
Prof. Dr. Carlos André Ornelas Ricart

E-Mail Website
Guest Editor
Department of Cell Biology, Institute of Biology, University of Brasília (UnB), Brasilia, Brazil
Interests: fungal biotechnology; proteomics; cell signaling; enzymology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The Special Issue "Biotechnological Applications of Fungi" aims to gather cutting-edge research and critical reviews focused on the versatile roles of fungi in industrial, environmental, agricultural, and biomedical biotechnology. Fungi are prolific producers of enzymes with high catalytic efficiency, valuable metabolites for pharmaceutical and agro-industrial uses, and are increasingly explored for environmental applications such as bioremediation and biocontrol. Their ability to degrade complex pollutants and participate in sustainable bioprocesses makes them essential biotechnological agents.

This Special Issue welcomes studies that explore (i) fungal enzymes and their industrial applications; (ii) the use of fungi in environmental remediation and waste valorization; (iii) the discovery and engineering of bioactive fungal metabolites; (iv) innovations in fungal fermentation processes; and (v) integrative omics approaches (genomics, transcriptomics, proteomics, metabolomics) that advance the understanding and optimization of fungal biotechnological functions.

Original research articles, reviews, and short communications are encouraged. We particularly invite submissions addressing novel fungal mechanisms, systems biology approaches, synthetic biology tools, and advances in fungal strain improvement.

We look forward to receiving your contributions to this exciting Special Issue in the Journal of Fungi.

Conflict of Interest Declaration

All Guest Editors declare no conflicts of interest related to company affiliations, company funding, equity participation, or industrial grants that may influence the editorial handling of submissions to this Special Issue.

Prof. Dr. Luis Henrique Ferreira Do Vale
Prof. Dr. Carlos André Ornelas Ricart
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Journal of Fungi is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • fungal biotechnology
  • enzyme production
  • bioremediation
  • secondary metabolites
  • fungal fermentation
  • omics technologies
  • proteomics
  • metabolomics
  • synthetic biology
  • fungal genetics

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Published Papers (2 papers)

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Research

14 pages, 8024 KB  
Article
The Fungus HL-29: A Promising Weed Pathogen with Bioherbicidal Potential and Crop Safety
by Lan Yang, Chao Peng, Haixia Zhu and Yongqiang Ma
J. Fungi 2026, 12(1), 17; https://doi.org/10.3390/jof12010017 - 25 Dec 2025
Viewed by 554
Abstract
The herbicidal efficacy and crop safety of Fusarium acuminatum strain HL-29, an endophytic fungus isolated from infected Amaranthus retroflexus in Qinghai Province, were evaluated. In vitro leaf assays demonstrated its pathogenicity against four broadleaf weeds, with efficacy ranked as follows: Elsholtzia densa = [...] Read more.
The herbicidal efficacy and crop safety of Fusarium acuminatum strain HL-29, an endophytic fungus isolated from infected Amaranthus retroflexus in Qinghai Province, were evaluated. In vitro leaf assays demonstrated its pathogenicity against four broadleaf weeds, with efficacy ranked as follows: Elsholtzia densa = Senecio vulgaris = Chenopodium album > Malva verticillata. Pot trials further confirmed that the HL-29 fermentate caused 100% disease incidence in S. vulgaris, C. album, and E. densa. Notably, the strain showed no pathogenicity toward seven major local crops, indicating excellent selectivity. Scanning electron microscopy (SEM) revealed key morphological changes during the infection process on C. album leaves. These results establish F. acuminatum HL-29 as a promising biocontrol candidate against broadleaf weeds in the Qinghai–Tibet Plateau, providing a theoretical foundation for developing alpine-adapted mycoherbicides. Full article
(This article belongs to the Special Issue Biotechnological Applications of Fungi)
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19 pages, 3727 KB  
Article
Extracellular Phosphate Availability Impacts Aspergillus terreus Itaconic Acid Fermentation via Biomass-Specific Product Yield
by Ákos P. Molnár, István Bakondi-Kovács, Vivien Bíró, Alexandra Márton, István S. Kolláth, Erzsébet Fekete, Norbert Ág, Erzsébet Sándor, András Csótó, Béla Kovács, Christian P. Kubicek and Levente Karaffa
J. Fungi 2026, 12(1), 14; https://doi.org/10.3390/jof12010014 - 25 Dec 2025
Viewed by 697
Abstract
Itaconic acid (IA) is an important bio-based platform chemical produced via submerged fermentation by the filamentous Ascomycete Aspergillus terreus. In this study, we examined the impact of initial phosphate concentration on IA production from D-glucose and D-xylose in optimized, manganese-limited fermentations. Nine [...] Read more.
Itaconic acid (IA) is an important bio-based platform chemical produced via submerged fermentation by the filamentous Ascomycete Aspergillus terreus. In this study, we examined the impact of initial phosphate concentration on IA production from D-glucose and D-xylose in optimized, manganese-limited fermentations. Nine phosphate concentrations ranging from 0.04 to 4 g L−1 were tested, and representative low (0.04 g L−1), optimal (0.1 g L−1), and high (0.8 g L−1) conditions were analyzed in detail in controlled, 6 L scale bioreactors. Phosphate availability primarily influenced biomass formation and the biomass-to-product ratio rather than directly affecting IA accumulation. Both lower- and higher-than-optimal phosphate concentrations decreased the volumetric and specific IA yields, while the highest productivity was observed at 0.1 g L−1. Expression of the aoxA gene, encoding the cyanide-resistant alternative oxidase (AOX), and AOX enzymatic activity were inversely correlated with extracellular phosphate concentration, consistent with a role in redox homeostasis under phosphate-limited conditions. In contrast, total respiration rates and pellet-type morphology remained unaffected. These findings indicate that phosphate acts mainly as a secondary modulator of IA fermentation performance through its influence on biomass formation, whereas other metabolic constraints play a more dominant role in controlling IA overflow in A. terreus. Full article
(This article belongs to the Special Issue Biotechnological Applications of Fungi)
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