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Nanoparticle-Based Biosensors and Their Applications

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Nanoscience".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 453

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Faculty of Foundry Engineering, AGH University of Science and Technology, 30-059 Krakow, Poland
Interests: physical chemistry; nanomaterials; molecular spectroscopy; biochemistry
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Special Issue Information

Dear Colleagues,

The study of (bio) molecules—including their detection, identification and structural characterization—is a fundamental objective across disciplines such as analytical chemistry, physics, and medicine. Scientists aim to observe the physical effects produced by individual molecules, as these effects reveal unique molecular properties that are often obscured when analyzing large ensembles due to averaging. We are seeking biosensors capable of detecting specific chemicals, combining a biological material or component with a physicochemical detector. The detector converts the signal resulting from the interaction of an analyte with a biological element into another signal that can be easily measured and quantified. Currently, there is significant interest in designing sensitive and specific biochemical nanosensors and protective layers. By selecting the right nano- and micro-structures as well as the right chemical elements, we can develop biosensors that are both biocompatible and selective. These biosensors interact with the target surface (at the solid/liquid interface) through adsorption and desorption processes. The interaction between biological and non-biological systems and metal surfaces is a key topic in biotechnology and biochemistry.

Prof. Dr. Edyta Proniewicz
Guest Editor

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Keywords

  • biosensors
  • metal nanoparticles (MNPs)
  • synthesis methods
  • molecular spectroscopy characteristics
  • molecular medicine
  • molecular biology

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Published Papers (1 paper)

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Research

19 pages, 2307 KB  
Article
SERS- and SEIRA-Based Characterization and Sensing of Highly Selective Bradykinin B2 Receptor Antagonists
by Edyta Proniewicz and Adam Prahl
Int. J. Mol. Sci. 2025, 26(16), 8089; https://doi.org/10.3390/ijms26168089 - 21 Aug 2025
Viewed by 303
Abstract
One of the major challenges in diagnosing various diseases, including neurological and neurodegenerative disorders, as well as carcinogenesis, is detecting unlabeled neurotransmitters. Surface-enhanced Raman spectroscopy (SERS) and surface-enhanced infrared spectroscopy (SEIRA) are promising methods for neurotransmitter biosensing and bioimaging. These methods are unique [...] Read more.
One of the major challenges in diagnosing various diseases, including neurological and neurodegenerative disorders, as well as carcinogenesis, is detecting unlabeled neurotransmitters. Surface-enhanced Raman spectroscopy (SERS) and surface-enhanced infrared spectroscopy (SEIRA) are promising methods for neurotransmitter biosensing and bioimaging. These methods are unique in that they are non-destructive and can identify molecular fingerprints. In this study, these methods were used to detect the following potent bradykinin (BK) antagonists: [D-Arg0,Hyp3,Thi5,D-Tic7,Oic8]BK, [D-Arg0,Hyp3,Thi5,D-Phe7,Thi8]BK, [D-Arg0,Hyp3,Igl5,D-Phe(5F)7,Oic8]BK, and [D-Arg0,Hyp3,Igl5,D-Igl7,Oic8]BK. The peptides were immobilized on a sensor surface consisting of silver (AgNPs) and gold (AuNPs) nanoparticles. These sensors have uniform particle sizes and small size distributions. Thanks to fast synthesis, easy handling, and reproducible results, these sensors enable routine testing. The vibrational structure of these peptides could not be determined using classical vibrational methods (Raman and IR) or surface-enhanced methods (SERS and SEIRA). This work presents the results of that research. Additionally, the SEIRA spectrum for BK or its analogs has not yet been published. This study presents research using SERS and SEIRA that shows that AgNP and AuNP sensors can detect the peptides under investigation. SERS is a more selective method than SEIRA because it allows for the differentiation of peptides based on the enhancement of certain bands in the SERS spectra. Furthermore, each peptide uniquely interacts with AuNPs, whereas all peptides bind to AgNPs via the C-terminus in different orientations. Consequently, the AuNP sensor is more selective than the AgNP sensor. Some bands were selected as markers for the sensing of specific peptides. Full article
(This article belongs to the Special Issue Nanoparticle-Based Biosensors and Their Applications)
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