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Viral Infections: Physiology, Pathophysiology, Pathogenesis, Diagnosis and Treatment, 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 28 February 2026 | Viewed by 1073

Special Issue Editors


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Guest Editor
Department of Pathology, Division of Microbiology, Wrocław University of Environmental and Life Sciences, Norwida 31, 50-375 Wrocław, Poland
Interests: virology; zoonoses; epidemiology; viral infection; cell cultures
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Special Issue Information

Dear Colleagues,

Viral infections have long been of interest to physicians and scientists around the world; however only the development of molecular techniques in recent decades has made it possible to understand many aspects of viral biology and their potential for transmission. Today, viral zoonotic diseases are becoming an increasing threat. This phenomenon is fostered by an increase in the size of both populations (human and animal) and the accompanying anthropogenic environmental degradation, as well as the need to convert wasteland into agricultural land and the interspecies transfer of pathogens resulting from climate change and the destruction of wildlife habitats and the environment.  In the fight against zoonoses, research indicating the mechanisms by which viruses cross interspecies barriers, their adaptability and mutagenic properties is extremely important.

We are pleased to invite you to submit articles describing and discussing the latest data on the pathogenesis, physiology, molecular biology, diagnosis and treatment of viral infections, with particular emphasis on research at the molecular level.

In this Special Issue, original research articles and reviews are welcome. Research areas may include (but are not limited to) the following:

  • zoonotic diseases;
  • interspecies barriers in viral infections;
  • virus mutations;
  • pathogenesis of viral infections;
  • oxidative stress in viral infections;
  • molecular diagnostic methods;
  • antiviral drugs/preparations;
  • viral diseases with epidemic potential;
  • physiological mechanisms of viral infections.

We look forward to receiving your applications.

Dr. Barbara Bażanów
Dr. Dominika Marta Stygar
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • viral infections
  • virus mutations
  • molecular diagnostic methods
  • antiviral drugs/preparations
  • viral diseases
  • physiological mechanisms

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Published Papers (2 papers)

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Research

17 pages, 2143 KB  
Article
CRISPR-Cas12a-Based Isothermal Detection of Mammarenavirus machupoense Virus: Optimization and Evaluation of Multiplex Capability
by Marina A. Kapitonova, Anna V. Shabalina, Vladimir G. Dedkov and Anna S. Dolgova
Int. J. Mol. Sci. 2025, 26(19), 9754; https://doi.org/10.3390/ijms26199754 - 7 Oct 2025
Viewed by 438
Abstract
Bolivian hemorrhagic fever (BHF) is a zoonotic disease caused by Mammarenavirus machupoense (MACV) featuring severe neurological and hemorrhagic symptoms and a high mortality rate. BHF is usually diagnosed by serological tests or real-time polymerase chain reaction (RT-PCR); these methods are often inaccessible in [...] Read more.
Bolivian hemorrhagic fever (BHF) is a zoonotic disease caused by Mammarenavirus machupoense (MACV) featuring severe neurological and hemorrhagic symptoms and a high mortality rate. BHF is usually diagnosed by serological tests or real-time polymerase chain reaction (RT-PCR); these methods are often inaccessible in endemic regions due to a lack of laboratory infrastructure, creating a demand for sensitive and rapid equipment-free alternatives. Here, we present an isothermal method for MACV nucleic acid detection based on the Cas12a-based DETECTR system combined with recombinase polymerase amplification (RPA) in a single tube: the RT-RPA/DETECTR assay. We demonstrate the possibility of using more than one primer set for the simultaneous detection of MACV genetic variants containing multiple point mutations. The method was optimized and tested using specially developed virus-like armored particles containing the target sequence. The multiplex RT-RPA/DETECTR method achieved a limit of detection of approximately 5 × 104 copies/ mL (80 aM) of armored particles. The method was validated using clinical samples spiked with virus-like particles. The assay proved to be selective and reliable in detecting certain nucleotide substitutions simultaneously. Full article
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19 pages, 1283 KB  
Article
Antioxidant and Antiviral Potential of Cold-Brewed and Cold-Concentrated Plant Extracts
by Paulina Janicka, Damian Maksimowski, Aleksandra Chwirot, Maciej Oziembłowski, Katarzyna Michalczyk, Agnieszka Nawirska-Olszańska, Piotr Poręba, Sylwia Baluta, Ewa Kaczmar, Dominika Stygar and Barbara Bażanów
Int. J. Mol. Sci. 2025, 26(19), 9617; https://doi.org/10.3390/ijms26199617 - 2 Oct 2025
Viewed by 486
Abstract
Norovirus (NoV) is a symptomatic virus that is the leading cause of gastrointestinal disease. It spreads easily through the fecal–oral route and contact with contaminated food or surfaces. Maintaining a high level of hygiene in food industry settings and refocusing food production on [...] Read more.
Norovirus (NoV) is a symptomatic virus that is the leading cause of gastrointestinal disease. It spreads easily through the fecal–oral route and contact with contaminated food or surfaces. Maintaining a high level of hygiene in food industry settings and refocusing food production on isolating and testing natural compounds that exhibit antimicrobial and antioxidant properties are important elements in preventing NoVs infection. This study evaluated plant extracts prepared by cold brew and cold concentrate techniques for their antioxidant and antiviral activity. The extracts obtained demonstrated high antioxidant activity, with notable variation depending on the plant material, ranging from moderate to very strong levels. Correspondingly, high antiviral potential was observed, reaching the nearly complete inactivation of the virus. Remarkably, the highest virucidal effects were already achieved at relatively elevated, but not maximal, antioxidant activity levels. The results of the study indicate that cold water extraction techniques allow for the obtention of plant extracts showing strong virus-inactivating activity and favorable antioxidant activity. Full article
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