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Omics Strategies in Male Reproduction
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Omics Strategies in Male Reproduction

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: closed (31 August 2022) | Viewed by 7713

Special Issue Editors

Prof. Dr. Monika Frączek

E-Mail Website
Guest Editor
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
Interests: male infertility; spermatogenesis; sperm physiology and pathology; environmental factors; semen quality; seminal biomarkers; male genital tract inflammation/infection; microbiota of male genital tract; oxidative stress; sperm mitochondria; sperm chromatin integrity; sperm epigenetics; seminal proteom; seminal metabolom
Dr. Marzena Skrzypczak-Zielińska

E-Mail Website
Guest Editor
Institute of Human Genetics, Polish Academy of Sciences, Poznan, Poland
Interests: seminal microbiota composition in health and disease; male infertility metagenomic research; high throughput techniques and omics data in male infertility studies

Special Issue Information

Dear Colleagues,

Male infertility has become a social and civilization disease, and started to be a serious challenge for public health. Due to limited predictive value of standard semen analysis in assessing the ability of the sperm to fertilize the oocyte, highly specialized scientific and diagnostic tools are needed to look for new non-invasive seminal biomarkers of male infertility, both in natural and assisted reproduction. Molecular research in sperm biology and male infertility is a very dynamic field of science. Omics-based studies using high-throughput technologies offer great potential for understanding the regulatory mechanisms that drive gene and protein expression during sperm development, detecting gene-environment or host-pathogen interactions underlying normal and pathological spermatogenesis. This research strategy is crucial to gain enough knowledge to envisage future diagnostic and therapeutic procedures to improve human reproductive health. 

The idea of the Special Issue is to advance the research on male infertility and the molecular background of spermatogenesis. Both original manuscripts and reviews discussing the genome, transcriptome, epigenome, proteome and metabolome data related to male factor fertility/infertility in humans and animal models are very welcome.

Prof. Dr. Monika Frączek
Dr. Marzena Skrzypczak-Zielińska
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • male infertility
  • normal and pathological spermatogenesis
  • seminal biomarkers
  • sperm chromatin
  • genomics
  • transcriptomics
  • epigenomics
  • proteomics
  • metabolomics
  • microbiome
  • 16S rRNA gene sequencing
  • miRNA
  • microarrays
  • next generation sequencing
  • cell in vitro studies
  • animal model studies

Published Papers (3 papers)

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Research

13 pages, 1729 KiB  
Article
Relative Abundance of Lipid Metabolites in Spermatozoa across Three Compartments
by Samuel Furse, Laura C. Kusinski, Alison Ray, Coralie Glenn-Sansum, Huw E. L. Williams, Albert Koulman and Claire L. Meek
Int. J. Mol. Sci. 2022, 23(19), 11655; https://doi.org/10.3390/ijms231911655 - 1 Oct 2022
Cited by 5 | Viewed by 3287
Abstract
Male fertility, as manifest by the quantity and progressive motility of spermatozoa, is negatively impacted by obesity, dyslipidaemia and metabolic disease. However, the relative distribution of lipids in spermatozoa and the two compartments which supply lipids for spermatogenesis (seminal fluid and blood serum) [...] Read more.
Male fertility, as manifest by the quantity and progressive motility of spermatozoa, is negatively impacted by obesity, dyslipidaemia and metabolic disease. However, the relative distribution of lipids in spermatozoa and the two compartments which supply lipids for spermatogenesis (seminal fluid and blood serum) has not been studied. We hypothesised that altered availability of lipids in blood serum and seminal fluid may affect the lipid composition and progressive motility of sperm. 60 men of age 35 years (median (range 20–45) and BMI 30.4 kg/m2 (24–36.5) under preliminary investigation for subfertility were recruited at an NHS clinic. Men provided samples of serum and semen, subject to strict acceptance criteria, for analysis of spermatozoa count and motility. Blood serum (n = 60), spermatozoa (n = 26) and seminal fluid (n = 60) were frozen for batch lipidomics analysis. Spermatozoa and seminal fluid had comparable lipid composition but showed marked differences with the serum lipidome. Spermatozoa demonstrated high abundance of ceramides, very-long-chain fatty acids (C20-22), and certain phospholipids (sphingomyelins, plasmalogens, phosphatidylethanolamines) with low abundance of phosphatidylcholines, cholesterol and triglycerides. Men with spermatozoa of low progressive motility had evidence of fewer concentration gradients for many lipid species between blood serum and spermatozoa compartments. Spermatozoa are abundant in multiple lipid species which are likely to contribute to key cellular functions. Lipid metabolism shows reduced regulation between compartments in men with spermatozoa with reduced progressive motility. Full article
(This article belongs to the Special Issue Omics Strategies in Male Reproduction)
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23 pages, 3103 KiB  
Article
Age-Dependent Variations in Functional Quality and Proteomic Characteristics of Canine (Canis lupus familiaris) Epididymal Spermatozoa
by Anna Zmudzinska, Jerzy Wisniewski, Piotr Mlynarz, Beata Olejnik and Marzena Mogielnicka-Brzozowska
Int. J. Mol. Sci. 2022, 23(16), 9143; https://doi.org/10.3390/ijms23169143 - 15 Aug 2022
Cited by 3 | Viewed by 1873
Abstract
Increased male age is associated with a significant reduction in semen quality. Little is known about the sperm proteome changes resulting from the aging process. This study aimed to investigate the relationship between the functional quality and proteome of epididymal spermatozoa of dogs [...] Read more.
Increased male age is associated with a significant reduction in semen quality. Little is known about the sperm proteome changes resulting from the aging process. This study aimed to investigate the relationship between the functional quality and proteome of epididymal spermatozoa of dogs that were differing in age. The study was conducted on 30 male dogs that were divided into three age groups. G1—12 to 41 months old, G2—42 to 77 months old, and G3—78 to 132 months old. The sperm samples were assessed using a computer-assisted semen analysis (CASA). The epididymal sperm proteins were analyzed using gel electrophoresis (SDS-PAGE), nano-liquid chromatography coupled to quadrupole time of flight mass spectrometry (NanoUPLC-Q-TOF/MS) and bioinformatic tools. The sperm quality parameters were significantly lower in older dogs. NanoUPLC-Q-TOF/MS identification resulted in 865 proteins that were found in the G1, 472 in G2, and 435 in G3. There were seven proteins that were present in all three age groups, and four of them (ACTB, CE10, NPC2, CRISP2) showed significant changes among the studied groups. Age-dependent variations were detected in the sperm proteome composition and were related to important metabolite pathways, which might suggest that several proteins are implicated in sperm maturation and could be potential aging biomarkers. Full article
(This article belongs to the Special Issue Omics Strategies in Male Reproduction)
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22 pages, 9405 KiB  
Article
In Silico Identification of lncRNAs Regulating Sperm Motility in the Turkey (Meleagris gallopavo L.)
by Jan Pawel Jastrzebski, Aleksandra Lipka, Marta Majewska, Karol G. Makowczenko, Lukasz Paukszto, Joanna Bukowska, Slawomir Dorocki, Krzysztof Kozlowski and Mariola Slowinska
Int. J. Mol. Sci. 2022, 23(14), 7642; https://doi.org/10.3390/ijms23147642 - 11 Jul 2022
Cited by 1 | Viewed by 1793
Abstract
Long non-coding RNAs (lncRNAs) are transcripts not translated into proteins with a length of more than 200 bp. LncRNAs are considered an important factor in the regulation of countless biological processes, mainly through the regulation of gene expression and interactions with proteins. However, [...] Read more.
Long non-coding RNAs (lncRNAs) are transcripts not translated into proteins with a length of more than 200 bp. LncRNAs are considered an important factor in the regulation of countless biological processes, mainly through the regulation of gene expression and interactions with proteins. However, the detailed mechanism of interaction as well as functions of lncRNAs are still unclear and therefore constitute a serious research challenge. In this study, for the first time, potential mechanisms of lncRNA regulation of processes related to sperm motility in turkey were investigated and described. Customized bioinformatics analysis was used to detect and identify lncRNAs, and their correlations with differentially expressed genes and proteins were also investigated. Results revealed the expression of 863 new/unknown lncRNAs in ductus deferens, testes and epididymis of turkeys. Moreover, potential relationships of the lncRNAs with the coding mRNAs and their products were identified in turkey reproductive tissues. The results obtained from the OMICS study may be useful in describing and characterizing the way that lncRNAs regulate genes and proteins as well as signaling pathways related to sperm motility. Full article
(This article belongs to the Special Issue Omics Strategies in Male Reproduction)
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