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The Role of Non-Coding RNAs in Female Reproduction

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: 20 December 2025 | Viewed by 434

Special Issue Editor


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Guest Editor
Department of Biomedical and Biotechnological Sciences, Section of Biology and Genetics “G. Sichel”, University of Catania, 95123 Catania, Italy
Interests: non-coding RNAs; female reproduction; reproductive aging; molecular biomarker discovery; extracellular vesicles; ceRNA network

Special Issue Information

Dear Colleagues, 

This Special Issue is focused on the crucial role that non-coding RNAs play in female reproduction. 

Non-coding RNAs regulate various processes, ranging from the earliest stages of oocyte development to the establishment and maintenance of pregnancy. Their dysregulation can be associated with both physiological and pathological conditions, comprising reproductive disorders, cancers and infertility. 

Their plethora of regulatory functions highlight the importance of understanding their role in reproductive biology and could lead to the development of novel therapeutics, as well as improve reproductive outcomes.

Dr. Angela Caponnetto
Guest Editor

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Keywords

  • microRNAs
  • long non-coding RNAs
  • circular RNAs
  • female reproduction
  • reproductive aging
  • molecular biomarkers
  • ceRNA network

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Published Papers (1 paper)

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Research

13 pages, 1414 KB  
Article
MicroRNAs Secreted by the Embryo in Spent Culture Medium Can Regulate mRNAs Involved in Endometrial Receptivity, Embryo Attachment, and Invasion
by Angela Caponnetto, Carmen Ferrara, Anna Fazzio, Luca Carli, Cristina Barbagallo, Michele Stella, Davide Barbagallo, Marco Ragusa, Michael Feichtinger, Cinzia Di Pietro and Rosalia Battaglia
Int. J. Mol. Sci. 2025, 26(18), 8879; https://doi.org/10.3390/ijms26188879 - 12 Sep 2025
Abstract
MicroRNAs, secreted by the embryo in blastocoel fluid (BF) and embryo spent culture medium (SCM), regulate important cellular pathways controlling the stemness of inner cell mass, trophectoderm differentiation, and the dialogue between blastocyst and maternal tissues. In recent years, their role as non-invasive [...] Read more.
MicroRNAs, secreted by the embryo in blastocoel fluid (BF) and embryo spent culture medium (SCM), regulate important cellular pathways controlling the stemness of inner cell mass, trophectoderm differentiation, and the dialogue between blastocyst and maternal tissues. In recent years, their role as non-invasive biomarkers of embryo quality has been deeply investigated. We compared the expression profiles of 96 microRNAs between BF and SCM from the same embryos, highlighting the differences between these two compartments. We found 10 and 6 microRNAs specifically expressed in BF and in SCM, respectively; 22 microRNAs significantly up-regulated in BF; and 2 significantly up-regulated in SCM. To investigate the role of SCM microRNAs in implantation, we focused on the microRNAs specifically expressed/up-regulated in SCM and absent in blank medium. We deepened our understanding of SCM microRNA’s biological role by building a network of miRNA–mRNA interaction within the signalling pathways crucial in embryo implantation success. We demonstrated that BF and SCM contain different sets of microRNAs playing different and unique roles in embryo implantation and development. Finally, we suggest that there is not a single “ideal” technique to identify the most competent embryo, but an integrated approach is needed to obtain informative results on the health of the embryo. Full article
(This article belongs to the Special Issue The Role of Non-Coding RNAs in Female Reproduction)
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