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Blueprints of Enzymatic Function and Structure in Biocatalysis

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 20 September 2025 | Viewed by 1405

Special Issue Editor


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Guest Editor
Department of Biochemical Sciences, Sapienza University of Rome, Rome, Italy
Interests: bioenergetics; biophysics; chemical biology; enzymology kinetics; molecular medicine; protein chemistry; structural biology
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Special Issue Information

Dear Colleagues,

In this Special Issue of the International Journal of Molecular Sciences, entitled "Blueprints of Enzymatic Function and Structure in Biocatalysis", we delve into the intricate relationship between enzyme structure, kinetics, and thermodynamics in the realm of biocatalysis. Enzymes, as nature's catalysts, play a vital role in accelerating biochemical reactions, and a deeper understanding of their structural and thermodynamic blueprints is essential for innovations in synthetic biology, pharmaceuticals, and industrial applications. This Special Issue aims to present cutting-edge research on enzyme engineering, mechanistic pathways, and the kinetic and thermodynamic principles that govern enzyme efficiency, shedding light on the design of more effective and sustainable biocatalysts.

Prof. Dr. Francesco Malatesta
Guest Editor

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Keywords

  • enzyme
  • biocatalysis
  • enzyme structure
  • enzyme kinetics and thermodynamics
  • enzyme engineering
  • synthetic biology applications
  • sustainable biocatalyst design

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Published Papers (1 paper)

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Review

18 pages, 3334 KiB  
Review
Exploiting Silica-Binding and Silica-Forming Proteins as Versatile Tools for One-Step Enzyme Immobilization on Siliceous Materials
by Gyun Taek Lim and Byung Hoon Jo
Int. J. Mol. Sci. 2025, 26(3), 1304; https://doi.org/10.3390/ijms26031304 - 3 Feb 2025
Cited by 1 | Viewed by 1159
Abstract
Enzyme immobilization has emerged as an essential technique in industrial applications of enzymes. Silica (SiO2) serves as a prominent support material for enzyme immobilization. Recent advancements have led to the development of various silica-binding proteins (SBPs) and silica-forming proteins (SFPs) that [...] Read more.
Enzyme immobilization has emerged as an essential technique in industrial applications of enzymes. Silica (SiO2) serves as a prominent support material for enzyme immobilization. Recent advancements have led to the development of various silica-binding proteins (SBPs) and silica-forming proteins (SFPs) that are invaluable tools in immobilizing enzymes on siliceous materials in a fast and simple manner. SBPs facilitate the immobilization of enzymes with controlled orientation on silica surfaces, while SFPs enable the biomimetic synthesis and encapsulation of enzymes within silica particles. In this review, we explore recent advances in the use of SBPs and SFPs in enzyme applications. We provide a comprehensive overview of their mechanisms and sequence characteristics relevant to enzyme immobilization. Additionally, we summarize the recombinant production and immobilization procedures for enzymes with SBPs or SFPs. We then categorize the available SBPs and SFPs into naturally occurring and artificially engineered types, presenting recent examples that demonstrate their utilization in enzyme immobilization. Our review highlights the strengths and limitations of various SBPs and SFPs and sheds light on future directions for the development of tailor-made biocatalytic silica. Full article
(This article belongs to the Special Issue Blueprints of Enzymatic Function and Structure in Biocatalysis)
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