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Advances in Molecular Biology on Mycobacteria: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 30 May 2025 | Viewed by 437

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National Reference Laboratory for Mycobacteria, Department of Infectious Diseases, National Institute of Health Doutor Ricardo Jorge (INSA), 1649-016 Lisbon, Portugal
Interests: mycobacteria
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Special Issue Information

Dear Colleagues,

First discovered in 1874 by Armauer Hansen, the genus Mycobacterium, with more than 220 species known to date, is the only genus of the Mycobacteriaceae family. Commonly, they are subdivided into three different groups—the members of the Mycobacterium tuberculosis complex (MTC), Mycobacterium leprae and non-tuberculous mycobacteria (NTM)—that can be classified into strict pathogens (MTC, M. leprae and some NTM) and opportunistic and saprophyte species (the majority of NTM). MTC members have already been declared as a world health problem and a public health threat; however, NTM are emerging as severe disease causative agents and there is a need to enhance their management, as their correct identification is essential for diagnosis and eventual outbreak detection.

For this Special Issue of IJMS, we intend to focus on recent developments regarding diagnosis, resistance detection and molecular surveillance using molecular methodologies and research on the members of the genus Mycobacterium. Original research papers and up-to-date review articles are welcome.

Dr. Rita Macedo
Guest Editor

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Keywords

  • genus Mycobacterium
  • non-tuberculous mycobacteria
  • Mycobacterium tuberculosis
  • Mycobacterium leprae
  • molecular diagnosis
  • molecular surveillance methodologies
  • molecular resistance prediction

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Published Papers (1 paper)

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Research

14 pages, 2234 KiB  
Article
A Quinoxaline 1,4-Dioxide Activates DNA Repair Systems in Mycobacterium smegmatis: A Transcriptomic Study
by Olga B. Bekker, Olesya O. Galanova, Aleksey A. Vatlin, Svetlana G. Frolova, Egor A. Shitikov, Dmitry A. Bespiatykh, Ksenia M. Klimina, Vladimir A. Veselovsky, Rustem A. Ilyasov, Svetlana V. Smirnova, Diana A. Reznikova, Nikita I. Kochetkov, Dmitry A. Maslov and Valery N. Danilenko
Int. J. Mol. Sci. 2025, 26(8), 3689; https://doi.org/10.3390/ijms26083689 - 14 Apr 2025
Viewed by 250
Abstract
In 2022, the World Health Organization reported that tuberculosis (TB) was the second leading cause of death globally from a single infectious agent following COVID-19. The development of new antitubercular agents with novel mechanisms of action for use in complex TB therapy is [...] Read more.
In 2022, the World Health Organization reported that tuberculosis (TB) was the second leading cause of death globally from a single infectious agent following COVID-19. The development of new antitubercular agents with novel mechanisms of action for use in complex TB therapy is considered a key approach to combating TB. In this study, we examined the gene expression profile of M. smegmatis when exposed to a promising antituberculosis agent, quinoxaline 1,4-dioxide (QdNO) 7-chloro-2-(ethoxycarbonyl)-3-methyl-6-(piperazin-1-yl)quinoxaline-1,4-dioxide-1 (LCTA-3368). We investigated how the bacterial response changed with different minimum inhibitory concentrations (MIC) (1/4 × MIC, 1/2 × MIC, and 1 × MIC) and durations (30 min and 90 min) of treatment with the drug. Our analysis revealed significant upregulation in genes involved in DNA repair and replication processes, as well as changes in the expression of 95 genes encoding proteins with oxidoreductase activity. We additionally showed that the concentration of reactive oxygen species increases in a dose-dependent manner upon exposure of M. smegmatis to LCTA-3368. These findings support the proposed mechanism of antibacterial action of QdNOs, which is associated with the formation of free radicals leading to DNA damage. Full article
(This article belongs to the Special Issue Advances in Molecular Biology on Mycobacteria: 2nd Edition)
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