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Molecular Research in Animal Reproduction

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 20 November 2025 | Viewed by 1028

Special Issue Editor


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Guest Editor
College of Animal Science and Technology, Key Laboratory of Animal Genetic, Breeding and Reproduction in Shaanxi Province, Northwestern A&F University, Yangling 712100, China
Interests: oocyte maturation; sperm preservation; environments and animal reproduction; animal biotechnology

Special Issue Information

Dear Colleagues,

Animal Reproduction is a process that involves generating animal offsprings to maintain breeds; it is also one of the critical steps to increasing the number of animals which are including the following processes: spermatogenesis, follicular development and oocyte maturation, fertilization, embryonic development and maternal recognition, the process of parturition, and so on. With the development of science and technology, each of the process of animal reproduction has been found to be deeply regulated by many factors and small molecular elements, such as endocrinology, cytokines, chemokines, epigenetic factors and miRNA, circle RNA, etc. However, the molecular mechanisms related to the animal reproduction process are quiet complex and need to be studied further. The aims of this Special Issue are to demonstrate the research progress regarding the molecular mechanisms of animal reproduction and to increase knowledge for research on animal reproduction while also trying to develop new methods or technologies to promote this process.

Prof. Dr. Zhongliang Jiang
Guest Editor

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Keywords

  • spermatogenesis
  • follicular atresia
  • oocyte maturation
  • granulosa cells
  • hormone regulation
  • autophagy
  • apoptosis
  • miRNA
  • signaling regulation

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Published Papers (1 paper)

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Research

17 pages, 8821 KiB  
Article
CircGRB14 Inhibits Proliferation and Promotes Apoptosis of Granulosa Cells in Chicken Follicle Selection Through Sponging miR-12264-3p and miR-6660-3p
by Huanqi Yang, Mengxiao Li, Beibei Zhang, Jinming Zhang, Yuxiang Shi, Tenghe Ma and Yanyan Sun
Int. J. Mol. Sci. 2025, 26(5), 2214; https://doi.org/10.3390/ijms26052214 - 28 Feb 2025
Viewed by 510
Abstract
The development and selection of ovarian follicles are essential for continuous egg production in chickens. Non-coding RNAs, particularly circular RNAs (circRNAs), play a critical regulatory role in follicle selection, a process heavily involving granulosa cells (GCs). In this study, we analyzed circRNA expression [...] Read more.
The development and selection of ovarian follicles are essential for continuous egg production in chickens. Non-coding RNAs, particularly circular RNAs (circRNAs), play a critical regulatory role in follicle selection, a process heavily involving granulosa cells (GCs). In this study, we analyzed circRNA expression profiles in small yellow follicles (SYFs) and large yellow follicles (LYFs) of Taihang chickens using RNA sequencing. We identified 14,586 circRNAs, with 57 showing differential expression (DE-circRNAs) between SYFs and LYFs. Functional enrichment analysis revealed that these DE-circRNAs are involved in key biological processes, including signal transduction, cell membrane formation, and nuclear enzymatic regulation. We focused on circGRB14, a circRNA derived from the growth factor receptor-bound protein 14 (GRB14) gene, as a potential regulator of follicle selection. Using qPCR, CCK-8 proliferation assays, and Annexin V/PI apoptosis analysis, we demonstrated that circGRB14 inhibits GC proliferation and promotes apoptosis. In contrast, miR-12264-3p and miR-6660-3p, validated as direct targets of circGRB14 via Dual-Luciferase Reporter assays, exhibited opposing effects by promoting GC proliferation and inhibiting apoptosis. These findings highlight the circGRB14-miR-12264-3p/miR-6660-3p axis as a key regulatory mechanism in GC dynamics during follicle selection. This study provides novel insights into the functional interplay between circRNAs and miRNAs in avian follicle development, offering potential targets for improving egg production in poultry. Full article
(This article belongs to the Special Issue Molecular Research in Animal Reproduction)
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