ijms-logo

Journal Browser

Journal Browser

Lipid Droplet Proteins Function and Metabolism

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 20 July 2025 | Viewed by 1004

Special Issue Editor


E-Mail Website
Guest Editor
Yeast Signalling Networks Group, i3s—Instituto de Investigação e Inovação em Saúde, Universidade do Port, Porto, Portugal
Interests: lipid and energy metabolism; lipid metabolic pathways; interorganelle membrane contact sites; organelle dysfunction in disease; aging; lipid-related diseases; molecular disease mechanisms in lipid disorders
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are excited to announce a forthcoming Special Issue, titled "Lipid Droplet Proteins Function and Metabolism", which is dedicated to the fascinating and rapidly evolving field of lipid droplet (LD) protein function and metabolism. Once thought of as passive fat storage organelles, these organelles are now recognized as dynamic hubs involved in numerous cellular processes, making them a topic of immense scientific interest that has gained momentum in the past decades.

This Special Issue aims to bring together cutting-edge research, insights, and discoveries related to LD proteins and their critical roles in cellular metabolism, signaling, and disease. It will provide a platform for researchers to share their novel findings on protein functions within LD metabolism, interactions, and the metabolic implications, expanding our understanding of LD biology.

We welcome contributions on various aspects, including lipid droplet proteomics, molecular mechanisms of lipid droplet formation, lipid metabolism, and the involvement of lipid droplets in diseases like obesity, diabetes, and lipid-related disorders. This Special Issue will serve as a valuable resource for researchers and scientists delving into the intricate world of these fascinating organelles and their multifaceted role in cellular metabolism. Join us in advancing this exciting field and submitting your groundbreaking work to contribute to this important scientific research area.

Dr. Vitor Teixeira
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • lipid droplets
  • LD protein
  • LD metabolism

Benefits of Publishing in a Special Issue

  • Ease of navigation: Grouping papers by topic helps scholars navigate broad scope journals more efficiently.
  • Greater discoverability: Special Issues support the reach and impact of scientific research. Articles in Special Issues are more discoverable and cited more frequently.
  • Expansion of research network: Special Issues facilitate connections among authors, fostering scientific collaborations.
  • External promotion: Articles in Special Issues are often promoted through the journal's social media, increasing their visibility.
  • e-Book format: Special Issues with more than 10 articles can be published as dedicated e-books, ensuring wide and rapid dissemination.

Further information on MDPI's Special Issue policies can be found here.

Published Papers (1 paper)

Order results
Result details
Select all
Export citation of selected articles as:

Research

15 pages, 5221 KiB  
Article
Phosphatidylcholine Cytidine Transferase α (CCTα) Affects LD Formation Through Fusion and Lipophagy in Bovine Mammary Epithelial Cells
by Jingna Yang, Yuxin Fan, Fangyuan Kang, Yanbin Yang, Yueying Wang, Yang Liu and Liqiang Han
Int. J. Mol. Sci. 2025, 26(5), 2135; https://doi.org/10.3390/ijms26052135 - 27 Feb 2025
Viewed by 394
Abstract
Phosphatidylcholine cytidine transferase α (CCTα) is a key rate-limiting enzyme in the CDP–choline pathway, the primary pathway for phosphatidylcholine (PC) synthesis in mammals. This study investigated the role of CCTα in lipid droplet (LD) formation, phospholipid synthesis, LD fusion, and lipophagy in bovine [...] Read more.
Phosphatidylcholine cytidine transferase α (CCTα) is a key rate-limiting enzyme in the CDP–choline pathway, the primary pathway for phosphatidylcholine (PC) synthesis in mammals. This study investigated the role of CCTα in lipid droplet (LD) formation, phospholipid synthesis, LD fusion, and lipophagy in bovine mammary epithelial cells (BMECs) through CCTα gene knockout (CCT-KO) and overexpression (CCT-OE). CCTα mRNA expression was significantly increased in bovine mammary gland tissue after lactation. In BMECs, CCTα was transferred from the nucleus to the endoplasmic reticulum and localized on LD surfaces in the presence of linoleic acid. Compared with normal BMECs (NC), CCTα knockout (CCT-KO) cells had significantly greater LD diameters (1.53 μm vs. 1.68 μm, p < 0.05), lower proportions of small LDs (<1 µm; 11.39% vs. 5.42%), and higher proportions of large LDs (>3 µm; 0.67% vs. 2.88%). In contrast, CCTα overexpression (CCT-OE) decreased the diameter of LDs to 1.18 μm (p < 0.01), increased the proportion of small LDs to 35.48%, and decreased the proportion of large LDs to 0.24%. CCTα knockout significantly decreased the PC content and the ratio of PC to PE, whereas CCTα overexpression increased the PC content and the ratio of PC to phosphatidyl ethanolamine (PE) (p < 0.05). The lipidomics analysis indicated that PC synthesis was significantly influenced by CCTα gene expression. Live cell observations showed that CCTα knockout promoted the fusion of small LDs into large LDs. In cells with CCT α overexpression, the expression of the microtubule-associated protein 1 light chain 3 (LC3) protein and the number of lysosomes was elevated, and the lysosomal phagocytosis of LDs was observed through transmission electron microscopy, thus indicating that CCTα overexpression enhanced lipophagy. In conclusion, these results suggest that CCTα plays a role in regulating LD formation by influencing PC synthesis, LD fusion, and lipophagy in BMECs. Full article
(This article belongs to the Special Issue Lipid Droplet Proteins Function and Metabolism)
Show Figures

Graphical abstract

Back to TopTop