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Application of Spectroscopic Methods in Molecular Biology Research

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Biology".

Deadline for manuscript submissions: 20 September 2026 | Viewed by 904

Special Issue Editor


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Guest Editor
Henryk Niewodniczanski Institute of Nuclear Physics, Polish Academy of Sciences, Krakow, Poland
Interests: nanotechnology; functional materials; molecular engineering; biomaterials; nanoelectronics; single-molecule magnets; nanocomposites
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Special Issue Information

Dear Colleagues,

Spectroscopic methods provide powerful, noninvasive tools for investigating the structure, dynamics, and interactions of biomolecules. Techniques such as infrared (IR), Raman, ultraviolet–visible (UV–Vis), fluorescence, nuclear magnetic resonance (NMR), and mass spectrometry offer complementary insights into proteins, nucleic acids, lipids, and complex biological assemblies. These techniques enable the elucidation of structure-function relationships that are central to molecular biology. Recent methodological advances have improved sensitivity and spatial and temporal resolution and enhanced the ability to monitor biomolecular processes under near-physiological conditions.

This Special Issue is dedicated to the application of spectroscopic methods in molecular biology research. We welcome contributions on studies that use spectroscopy to investigate biomolecular interactions, monitor conformational changes, or characterize biomaterials for therapeutic and diagnostic applications. We particularly encourage submissions that highlight methodological innovations, integration with computational approaches, and/or multi-modal strategies that expand the scope of spectroscopy in biology.

Prof. Dr. Łukasz Laskowski
Guest Editor

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Keywords

  • spectroscopy
  • molecular characterization
  • biomaterials
  • computational chemistry
  • structure–property relationships
  • biomolecular interactions
  • biomedical applications

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Published Papers (1 paper)

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Research

30 pages, 2714 KB  
Article
Combined Approach of Chromatographic Fractionation and Raman Spectroscopy for Metabolite Profiling of Enterobacter spp. Supernatant
by Elizaveta Denisova, Anastasia Avdyusheva, Vera Vasilieva, Elizaveta Tyshchuk, Polina Grebenkina, Andrey Korenevsky, Ivan Chelibanov, Vladimir Chelibanov, Areg Totolian, Lyudmila Kraeva and Dmitry Sokolov
Int. J. Mol. Sci. 2026, 27(3), 1564; https://doi.org/10.3390/ijms27031564 - 5 Feb 2026
Viewed by 633
Abstract
The secretome of ESKAPE pathogens contains numerous bioactive molecules that play a key role in pathogenesis and the formation of an immunosuppressive microenvironment. However, analyzing this complex chemical composition presents significant methodological challenges. In this study, we propose a combined approach integrating chromatographic [...] Read more.
The secretome of ESKAPE pathogens contains numerous bioactive molecules that play a key role in pathogenesis and the formation of an immunosuppressive microenvironment. However, analyzing this complex chemical composition presents significant methodological challenges. In this study, we propose a combined approach integrating chromatographic fractionation of cell-free supernatants with Raman spectroscopy to deconstruct the secretome of the clinically relevant Gram-negative pathogen—Enterobacter spp. Chromatographic separation of the Enterobacter spp. supernatant into seven fractions reduced spectral congestion and enabled identification of fraction 3 as having a unique metabolite profile, enriched in peptides (including tryptophan- and tyrosine-containing structures), nucleic acids, polysaccharides, and putative glutathione-like compounds. Notably, fraction 3 lacked markers of phenylalanine and sterol-like lipids, highlighting its distinct composition. Compared to conventional mass spectrometry and nuclear magnetic resonance, our hybrid strategy offers minimal sample preparation, preserves sample integrity for repeated analysis, avoids ionization bias, and is fully compatible with aqueous biological matrices—critical advantages for profiling labile or low-abundance metabolites in native secretomes. These findings demonstrate that the combination of preparative chromatography and Raman spectroscopy effectively resolves complex bacterial secretomes and identifies fractions potentially carrying key virulence or signaling functions. Full article
(This article belongs to the Special Issue Application of Spectroscopic Methods in Molecular Biology Research)
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