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Plant Nitrogen Metabolism: Current Status and Future Directions

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Plant Sciences".

Deadline for manuscript submissions: closed (20 May 2025) | Viewed by 1288

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Departamento de Botánica, Ecología y Fisiología Vegetal, Instituto Andaluz de Biotecnología, Campus de Rabanales, Universidad de Córdoba, 14071 Córdoba, Spain
Interests: nucleotides; ureides; ribonucleases; senescence; germination
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Special Issue Information

Dear Colleagues,

Nitrogen is a vital element for plants since it is a component of some important molecules such as proteins, nucleic acids, chlorophylls, and some secondary metabolites. A reduced availability of nitrogen for plants leads to a reduction in plant growth and losses in crop yield. Nitrogen is mainly acquired as nitrate and/or ammonium from soil, and for its efficient assimilation, plants have developed sophisticated and regulated mechanisms of uptake and assimilation. In agriculture nitrogen is supplemented to plants using synthetic fertilizers, which have led to some important environmental challenges. Legumes can use the atmospheric nitrogen as nitrogen source thanks to the establishment of symbiotic association with certain root nodule-forming bacteria. This has a great interest both economic and environmental since nitrogen fixation reduce the use of fertilizers in agriculture.

Despite its importance to plant development and crop yield, many aspects of nitrogen metabolism in plants are still poorly understood. This Special Issue will focus on all the aspects related to nitrogen metabolism, including uptake, assimilation, transport, catabolism, recycling, etc. Articles with new approaches to increase crop yield in the near future, especially in changing conditions are welcome. Original research papers and reviews are welcome.

Dr. Pedro Piedras
Guest Editor

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Keywords

  • nitrate
  • ammonium
  • amides, ureides
  • nitrogen fixation
  • reactive nitrogen species
  • nitrogen signalling
  • nitrogen molecules in response to stress
  • nitrogen molecules in mobilization situations
  • sustainable agriculture

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Published Papers (1 paper)

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Research

21 pages, 7883 KiB  
Article
Transcriptomic Analysis to Understand the Nitrogen Stress Response Mechanism in BNI-Enabled Wheat
by Chandra Nath Mishra, Sushma Kumari Pawar, Swati Sharma, Arun Thakur, Sabhyata Sabhyata, Shubham Mishra, Satish Kumar, Om Prakash Gupta, Arun Kumar Joshi and Ratan Tiwari
Int. J. Mol. Sci. 2025, 26(10), 4610; https://doi.org/10.3390/ijms26104610 - 12 May 2025
Viewed by 461
Abstract
A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they are [...] Read more.
A comparative transcriptomic analysis was conducted for the nitrogen-efficient (BNI-Munal) and derivative parent Munal wheat genotypes to unravel the gene expression patterns across four nitrogen levels (0%, 50%, 75%, and 100%). Analyzing the genes of BNI-enabled wheat helps us understand how they are expressed differently, which heavily influences BNI activity. Grain yield and 1000-grain weight were higher in BNI Munal than in Munal. All the other traits were similar in performance. Varying nitrogen dosages led to significant differences in gene expression patterns between the two genotypes. Genes related to binding and catalytic activity were prevalent among molecular functions, while genes corresponding to cellular anatomical entities dominated the cellular component category. Differential expression was observed in 371 genes at 0%N, 261 genes at 50%N, 303 genes at 75%N, and 736 genes at 100%N. Five unigenes (three upregulated and two downregulated) were consistently expressed across all nitrogen levels. Further analysis of upregulated unigenes identified links to the NrpA gene (involved in nitrogen regulation), tetratricopeptide repeat-containing protein (PPR), and cytokinin dehydrogenase 2. Analysis of downregulated genes pointed to associations with the Triticum aestivum 3BS-specific BAC library, which encodes the NPF (Nitrate and Peptide Transporter Family) and the TaVRN gene family (closely related to the TaNUE1 gene). The five unigenes and one unigene highlighted in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways were validated in Munal and BNI Munal. The results obtained will enhance our understanding about gene expression patterns across different nitrogen levels in BNI wheat and help us breed wheat varieties with the BNI trait for improved NUE. Full article
(This article belongs to the Special Issue Plant Nitrogen Metabolism: Current Status and Future Directions)
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