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Advances in Yeast Engineering and Stress Responses

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Microbiology".

Deadline for manuscript submissions: 20 June 2026 | Viewed by 618

Special Issue Editors


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Guest Editor
Institut de Biotecnologia i Biomedicina, Departament de Bioquímica i Biologia Molecular, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Spain
Interests: protein phosphorylation; stress signaling; gene expression; transcriptomics; heterologous protein expression; S. cerevisiae; K. phafii
Special Issues, Collections and Topics in MDPI journals
Department of Chemical, Biological and Environmental Engineering, Universitat Autònoma de Barcelona, 08193 Bellaterra, Catalonia, Spain
Interests: industrial biotechnology; synthetic biology; metabolic engineering; bioprocess engineering; recombinant protein production; metabolite production; yeast; K. phaffii
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Microorganisms such as yeasts and filamentous fungi are exposed to changing environmental conditions (temperature, osmotic pressure, pH, chemicals, etc.). Moreover, they are often required to perform under harsh/non-optimal physiological conditions for the efficient industrial production of value-added products. To cope with these stress situations, they have developed adaptive responses that often involve extensive remodeling of gene expression, as well as metabolic and morphological alterations.  Understanding how stress signals are sensed and deciphering the key molecular pathways leading to these responses provide fundamental knowledge for engineering stress-resistant yeast strains to improve industrial bioproduction processes.

This Special Issue aims to combine current approaches to elucidating the molecular basis of the stress response in yeast with strategies that take advantage of this knowledge in order to generate more robust, efficient and productive strains and design optimized fermentation processes that minimize or exploit these stress responses. This combined knowledge will make it possible to achieve cheaper and more efficient and sustainable industrial processes.

Dr. Joaquin Ariño
Dr. Pau Ferrer
Guest Editors

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Keywords

  • stress tolerance
  • cellular adaptation
  • nutrient limitation
  • gene expression
  • metabolic engineering
  • synthetic biology
  • strain robustness
  • fermentation efficiency
  • bioprocess optimization

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Published Papers (2 papers)

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Research

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31 pages, 3453 KB  
Article
Simulated Microgravity-Induced Changes in SUMOylation and Protein Expression in Saccharomyces cerevisiae
by Jeremy A. Sabo and Steven D. Hartson
Int. J. Mol. Sci. 2026, 27(1), 42; https://doi.org/10.3390/ijms27010042 (registering DOI) - 19 Dec 2025
Abstract
Microgravity during space travel induces significant regulatory changes in the body, posing health risks for astronauts, including alterations in cell morphology and cytoskeletal integrity. The Small Ubiquitin-like Modifier (SUMO) is crucial for cellular adaptation, regulating DNA repair, cytoskeletal dynamics, cell division, and protein [...] Read more.
Microgravity during space travel induces significant regulatory changes in the body, posing health risks for astronauts, including alterations in cell morphology and cytoskeletal integrity. The Small Ubiquitin-like Modifier (SUMO) is crucial for cellular adaptation, regulating DNA repair, cytoskeletal dynamics, cell division, and protein turnover—all processes affected by microgravity. To determine the extent to which SUMO mediates the cellular response to microgravity stress, Saccharomyces cerevisiae cells were cultured under normal gravity and simulated microgravity (SMG) in rotating wall vessels. After 12 h of culture, we investigated changes in SUMO modified proteins and protein expression. We identified 347 SUMOylated proteins, 18 of which demonstrated a 50% change in abundance under SMG. Of 3773 proteins identified, protein expression for 34 proteins decreased and 8 increased by over 50% in SMG (p < 0.05). Differentially expressed proteins represented changes in cellular processes for DNA repair, cell division, histone modification, and cytoskeleton regulation. These findings underscore the pivotal role of SUMOylation in orchestrating cellular adaptation to the unique stress of microgravity, revealing potential targets for mitigating spaceflight-induced health risks. Full article
(This article belongs to the Special Issue Advances in Yeast Engineering and Stress Responses)

Review

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25 pages, 1057 KB  
Review
Transcriptional Responses to Alkaline pH Across Fungi: Common and Differential Features, and Biotechnological Applications
by Joaquín Ariño
Int. J. Mol. Sci. 2025, 26(23), 11450; https://doi.org/10.3390/ijms262311450 - 26 Nov 2025
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Abstract
The transcriptional response to alkalinization in Saccharomyces cerevisiae, Aspergillus nidulans and Candida albicans raised the interest of the scientific community many years ago for diverse reasons, and the underlying signaling pathways have been elucidated in these organisms in detail. Within the last [...] Read more.
The transcriptional response to alkalinization in Saccharomyces cerevisiae, Aspergillus nidulans and Candida albicans raised the interest of the scientific community many years ago for diverse reasons, and the underlying signaling pathways have been elucidated in these organisms in detail. Within the last few years, transcriptomic data for other fungal species have become available, although in most cases little is known about the molecular basis controlling their adaptive response. The objective of this work is to provide an overview on how different fungi remodel their gene expression in response to environmental alkalinization, highlighting the similitudes and differences among them. Microbial stress-responsive promoters have been considered useful tools for biotechnological applications, such as expression of recombinant proteins of industrial interest. Recent work, emphasizing the usefulness of alkaline pH-inducible promoters for heterologous protein production, will also be discussed. Full article
(This article belongs to the Special Issue Advances in Yeast Engineering and Stress Responses)
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