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Genetic and Epigenetic Toxicology

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Genetics and Genomics".

Deadline for manuscript submissions: closed (30 October 2024) | Viewed by 1115

Special Issue Editor


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Guest Editor
Department of Biochemistry and Molecular Biology, University of Florida, Gainesville, FL 32610, USA
Interests: genome stability; DNA repair; DNA replication; oxidative stress; oxidative DNA damage
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Environmental hazards and certain harmful substances promote deleterious modifications in cells and affect the integrity of both genetic information and epigenetic states. Being exposed to many chemical and biological agents can result in genomic instabilities, genetic mutations, chromosomal damage, and epigenetic alterations, which lead to many human maladies such as neurodegenerative diseases, metabolic disorders, and cancer.

This Special Issue provides a comprehensive overview from both genetic and epigenetic aspects to identify the effects of environmental toxicants and carcinogens on human health. In this Special Issue of the International Journal of Molecular Sciences, the main topic will be “Genetic and Epigenetic Toxicology”.

This Special Issue is intended to contribute to the field with original research articles or reviews putting in the spotlight the relevance of genetic and epigenetic toxicology in cancer cells, chemotherapy, possibly providing indications about future innovative therapeutic approaches, and the novel findings and mechanisms in wider clinical applications. Moreover, reports about novel, effective testing methods in improving the diagnosis and treatment of many diseases are also welcome.

Dr. Melike Caglayan
Guest Editor

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Keywords

  • toxicology
  • epigenetic toxicity
  • DNA mutations
  • environmental mutagens
  • oxidative stress
  • chemical hazards
  • genotoxic carcinogens
  • cancer

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Published Papers (1 paper)

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Research

12 pages, 1507 KiB  
Article
Site Matters: Differences in Gene Expression Profiles Along the Bovine Rumen Papilla During Subacute Rumen Acidosis
by Arife Sener-Aydemir, Franziska Dengler, Filip Larsberg, Raul Rivera-Chacon, Ezequias Castillo-Lopez, Qendrim Zebeli and Susanne Kreuzer-Redmer
Int. J. Mol. Sci. 2024, 25(22), 12303; https://doi.org/10.3390/ijms252212303 - 16 Nov 2024
Viewed by 822
Abstract
Subacute rumen acidosis (SARA) is a significant concern in dairy cattle fed grain-rich diets. To elucidate the underlying pathophysiological mechanisms, ruminal papilla biopsies are often used. This study aimed to assess how the sampling site along the ruminal papilla influences gene expression profiles [...] Read more.
Subacute rumen acidosis (SARA) is a significant concern in dairy cattle fed grain-rich diets. To elucidate the underlying pathophysiological mechanisms, ruminal papilla biopsies are often used. This study aimed to assess how the sampling site along the ruminal papilla influences gene expression profiles in rumen epithelium during SARA. Rumen biopsies from five ruminal-cannulated non-lactating Holstein cows were collected during feeding of a forage diet (FD) and seven (wk1) and 21 days (wk3) after transition to high-grain (HG) feeding. Gene expression in apical (AP), basal (BP), and total length (TP) papillae were compared using RT-qPCR. Significant diet-induced effects were observed in AP for DSG1 (wk3, p = 0.0317), ZO1 (wk1 and wk3, p = 0.0159), GLUT3 (wk3, p = 0.0159), TLR4 (wk3, p = 0.0635), and NFKB (wk1, p = 0.0159), but hardly in BP or TP. Within wk1, TP showed higher transcript levels of ZO1 and TLR4 (p = 0.0079) and SGLT1 (p = 0.0317) compared to AP and BP independently from diet effects. These findings suggest that the apical parts of rumen papillae biopsies are most suitable for gene expression analyses to investigate diet-induced effects on rumen physiology and underscore the importance of considering the sampling site for accurate gene expression studies in rumen epithelium during SARA, providing valuable insights for future research and diagnostic approaches in managing rumen health in dairy cattle. Full article
(This article belongs to the Special Issue Genetic and Epigenetic Toxicology)
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