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Sarcomere Function in Health and Disease 2.0: New Insights from Traditional to Advanced Animal-Free Studies

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Molecular Pathology, Diagnostics, and Therapeutics".

Deadline for manuscript submissions: 15 May 2024 | Viewed by 1810

Special Issue Editor

Department Chemistry Biomedical Sciences, Linnaeus University, SE-39182 Kalmar, Sweden
Interests: myosin; actomyosin; gelsolin; contracting muscle; enzyme kinetics; myosin mitochondrial transport; protein expression and purification; single molecule biophysics
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

We are launching a Special Issue focusing on muscle cell contractility. Indeed, one very important function of muscle cells is producing a force sufficient to allow for contraction. Such contraction requires the coordinated action of well-structured proteins named sarcomeric proteins.

As the exact function of these sarcomeric proteins (and how they interact with neighbouring molecules) to allow for muscle cell contraction is only partially known, we seek original research papers and review articles on this topic. We also hope to receive articles that better explain how these sarcomeric proteins can be dysfunctional in the context of muscle diseases.

Dr. Marko Usaj
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

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Keywords

  • myosin
  • actin
  • titin
  • z-disc
  • m-line
  • skeletal muscle
  • heart
  • contraction
  • myopathy
  • muscular dystrophy

Published Papers (1 paper)

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Research

16 pages, 7574 KiB  
Article
Changes in the Mechanical Properties of Fast and Slow Skeletal Muscle after 7 and 21 Days of Restricted Activity in Rats
by Sergey A. Tyganov, Svetlana P. Belova, Olga V. Turtikova, Ivan M. Vikhlyantsev, Tatiana L. Nemirovskaya and Boris S. Shenkman
Int. J. Mol. Sci. 2023, 24(4), 4141; https://doi.org/10.3390/ijms24044141 - 18 Feb 2023
Cited by 1 | Viewed by 1418
Abstract
Disuse muscle atrophy is usually accompanied by changes in skeletal muscle structure, signaling, and contractile potential. Different models of muscle unloading can provide valuable information, but the protocols of experiments with complete immobilization are not physiologically representative of a sedentary lifestyle, which is [...] Read more.
Disuse muscle atrophy is usually accompanied by changes in skeletal muscle structure, signaling, and contractile potential. Different models of muscle unloading can provide valuable information, but the protocols of experiments with complete immobilization are not physiologically representative of a sedentary lifestyle, which is highly prevalent among humans now. In the current study, we investigated the potential effects of restricted activity on the mechanical characteristics of rat postural (soleus) and locomotor (extensor digitorum longus, EDL) muscles. The restricted-activity rats were kept in small Plexiglas cages (17.0 × 9.6 × 13.0 cm) for 7 and 21 days. After this, soleus and EDL muscles were collected for ex vivo mechanical measurements and biochemical analysis. We demonstrated that while a 21-day movement restriction affected the weight of both muscles, in soleus muscle we observed a greater decrease. The maximum isometric force and passive tension in both muscles also significantly changed after 21 days of movement restriction, along with a decrease in the level of collagen 1 and 3 mRNA expression. Furthermore, the collagen content itself changed only in soleus after 7 and 21 days of movement restriction. With regard to cytoskeletal proteins, in our experiment we observed a significant decrease in telethonin in soleus, and a similar decrease in desmin and telethonin in EDL. We also observed a shift towards fast-type myosin heavy chain expression in soleus, but not in EDL. In summary, in this study we showed that movement restriction leads to profound specific changes in the mechanical properties of fast and slow skeletal muscles. Future studies may include evaluation of signaling mechanisms regulating the synthesis, degradation, and mRNA expression of the extracellular matrix and scaffold proteins of myofibers. Full article
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