Micropropagation and Cultivation of Ornamental Species

A special issue of Horticulturae (ISSN 2311-7524). This special issue belongs to the section "Floriculture, Nursery and Landscape, and Turf".

Deadline for manuscript submissions: 31 July 2026 | Viewed by 2113

Special Issue Editors


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Guest Editor
Department of Ornamental Plants and Garden Art, Faculty of Horticulture and Biotechnology, University of Agriculture in Krakow, Krakow, Poland
Interests: ornamental plants; micropropagation; cryopreservation; plants quality assessment; therapeutic horticulture; landscape plant utilization
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Guest Editor
Department of Ornamental Plants and Garden Art, University of Agriculture in Krakow, 31-120 Kraków, Poland
Interests: in-vitro propagation; plant biotechnology; LED lighting applications; micropropagation protocols optimization; plant cryopreservation; conservation of genetic resources; controlled-environment horticulture; phytohormones; ornamental plants cultivation; plants physiological quality

Special Issue Information

Dear Colleagues,

The global ornamental horticulture sector—spanning annuals, perennials, woody nursery stock, potted plants, and cut flowers—relies heavily on efficient and reliable micropropagation systems. This Special Issue invites high-quality research that advances both the science and practice of propagating ornamental species using tissue culture. We welcome contributions addressing generative and vegetative in vitro propagation, innovations in tissue culture and micropropagation, and studies that elucidate the physiological, biochemical, or molecular mechanisms underlying propagation success. Submissions may explore basic and applied aspects, including rooting biology, acclimatization, stress mitigation, genotype-specific responses, and novel solutions as bioreactors, biostimulants, or growth regulators. We are also particularly interested in studies offering solutions to commercial bottlenecks, improving propagation efficiency, production uniformity, quality, and cost-effectiveness. Given the diverse climatic, cultural, and economic contexts in which ornamental crops are cultivated worldwide, comparative or regionally focused research is equally encouraged. This Special Issue aims to showcase cutting-edge approaches that support sustainable, high-quality ornamental plant production and inspire further innovation in this dynamic field.

Dr. Bożena Szewczyk-Taranek
Prof. Dr. Bożena Pawłowska
Guest Editors

Manuscript Submission Information

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Keywords

  • micropropagation
  • tissue culture
  • vegetative propagation
  • seed propagation
  • ornamental plants
  • nursery production
  • greenhouse cultivation
  • rooting and acclimatization
  • growth regulators
  • production efficiency

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Published Papers (2 papers)

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Research

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17 pages, 3204 KB  
Article
In Vitro Propagation System for Proiphys amboinensis Using Twin-Scale Explants and Genetic Fidelity Assessment
by Kornkanok Chamchusri, Piyanuch Sornchai, Pitchaporn Wannitikul, Panumart Rithichai and Yaowapha Jirakiattikul
Horticulturae 2026, 12(3), 317; https://doi.org/10.3390/horticulturae12030317 - 6 Mar 2026
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Abstract
Proiphys amboinensis has considerable potential as a commercial ornamental plant due to its attractive foliage, distinctive flowers, and long flowering period. This study established an in vitro micropropagation protocol and evaluated the genetic fidelity of regenerated bulblets using Inter Simple Sequence Repeat (ISSR) [...] Read more.
Proiphys amboinensis has considerable potential as a commercial ornamental plant due to its attractive foliage, distinctive flowers, and long flowering period. This study established an in vitro micropropagation protocol and evaluated the genetic fidelity of regenerated bulblets using Inter Simple Sequence Repeat (ISSR) markers and flow cytometry. Twin-scale explants were cultured on Murashige and Skoog (MS) medium supplemented with 0, 0.5, 1.0, and 2.0 mg/L N6-benzyladenine (BA) for 12 weeks. Bulblet formation efficiency ranged from 60.00 ± 16.33% to 70.00 ± 11.55%, with no significant differences among treatments. A significant increase in bulblet number was observed at 1.0 mg/L BA compared with the control and 0.5 mg/L BA; however, bulblet fresh weight did not differ significantly among these treatments. Sucrose concentrations (30–90 g/L) had no significant effects on bulblet weight and diameter. Root induction was evaluated using indole-3-butyric acid (IBA) and α-naphthaleneacetic acid (NAA) at concentrations of 0–1.0 mg/L, with 0.5 mg/L IBA identified as the optimal treatment. Following acclimatization, regenerated bulblets exhibited high survival rates (90–100%). ISSR and flow cytometric analyses revealed no detectable genetic variation, with a consistent genome size between regenerated bulblets and the mother plants, indicating high genetic uniformity. The protocol provides a micropropagation system for P. amboinensis with high genetic fidelity, supporting its commercial and research potential. Full article
(This article belongs to the Special Issue Micropropagation and Cultivation of Ornamental Species)
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Review

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24 pages, 3485 KB  
Review
Micropropagation, Somatic Embryogenesis, and Haploid Induction in Passiflora: Advances, Biological Constraints, and Breeding Prospects
by Mohammad Gul Arabzai, Ting Wu, Nazir Khan Mohammadi, Niaz Mohammad Inqilabi, Omotola Adebayo Olunuga, Yuan Qin and Lulu Wang
Horticulturae 2026, 12(4), 497; https://doi.org/10.3390/horticulturae12040497 - 19 Apr 2026
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Abstract
The genus Passiflora includes species important for fruit production, ornamental value, and breeding programs. Conventional methods, such as seed propagation and vegetative cuttings, face challenges like genetic heterogeneity, pathogen transmission, and long juvenile phases, limiting large-scale cultivation and breeding efficiency. In vitro culture [...] Read more.
The genus Passiflora includes species important for fruit production, ornamental value, and breeding programs. Conventional methods, such as seed propagation and vegetative cuttings, face challenges like genetic heterogeneity, pathogen transmission, and long juvenile phases, limiting large-scale cultivation and breeding efficiency. In vitro culture technologies are essential for clonal propagation, germplasm conservation, and improving Passiflora species using biotechnology. This review critically evaluates current progress in micropropagation and regeneration systems in Passiflora spp. and examines the prospects of haploid and doubled haploid technologies as future breeding tools. Unlike previous reviews, which primarily focus on summarizing tissue culture protocols, this study integrates regeneration biology, developmental constraints, and emerging biotechnological approaches to provide a broader framework for research. Additionally, this review offers a comparative analysis of various regeneration systems across Passiflora species and highlights the challenges of genotype-dependent methods. By synthesizing recent advancements in haploid technology, it provides new insights into the potential for accelerating breeding programs in Passiflora, a field where robust protocols are still lacking. Full article
(This article belongs to the Special Issue Micropropagation and Cultivation of Ornamental Species)
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