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Fishes
Special Issues
Application of Biological Technologies in Aquatic Animal Genetic Breeding
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Application of Biological Technologies in Aquatic Animal Genetic Breeding

A special issue of Fishes (ISSN 2410-3888). This special issue belongs to the section "Genetics and Biotechnology".

Deadline for manuscript submissions: closed (20 February 2023) | Viewed by 3089

Special Issue Editors

Prof. Dr. Hongyu Ma

E-Mail Website
Guest Editor
Marine Biology Institute, Shantou University, Shantou 515063, China
Interests: genetics and breeding; sex determination and differentiation; early development; population genetics and phylogeny; aquaculture
Special Issues, Collections and Topics in MDPI journals
Dr. Zhenkui Qin

E-Mail Website
Guest Editor
Ministry of Education Key Laboratory of Marine Genetics and Breeding, College of Marine Life Sciences, Ocean University of China, Qingdao 266003, China
Interests: reproduction; sex determination and differentiation; development; reproductive regulation; sex control; molecular mechanisms; shellfish
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Aquatic animals, including fishes, crustaceans, shellfishes, etc., have made great contributions to the development of aquaculture industry, and provided premium protein resources for human beings. Biotechnologies have been and will be continuously applied in the breeding of aquatic animals in order to improve the important production traits. This Special Issue focuses on the application of biological technologies in aquaculture organisms to explore the regulatory mechanisms and to improve the productive performances of the related production traits, such as growth, fecundity, disease resistance, etc., as well as the development of new breeding techniques, such as sex manipulation, genome modification, etc., or investigation of the traditional/quantitative/molecular genetics in breeding.  

Gene editing and transgene techniques have proven to be a powerful tool for biological characteristics manipulation and genetic modification, as well as the trait-related regulatory genes discovery. Any research on the utilization of gene editing platforms in improving/exploring such aspects are preferred. In addition, a combination of various biotechnologies to elucidate the mechanisms of the important traits-regulation are welcomed.

The advancement of next-generation sequencing combining with the continuous reduction in cost have greatly accelerated its utilization in genetic information elucidation. Research related to genome, transcriptome, epigenome, etc., as well as methodology in relevant areas are appreciated.

Prof. Dr. Hongyu Ma
Dr. Zhenkui Qin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Fishes is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • aquaculture
  • breeding
  • genetics
  • reproduction
  • development

Published Papers (1 paper)

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Research

22 pages, 5700 KiB  
Article
The Effect of Knocked-Down Anti-Müllerian Hormone mRNA on Reproductive Characters of Male Nile Tilapia (Oreochromis niloticus) through Inhibition of the TGF-Beta Signaling Pathway
by Yue Yan, Yifan Tao, Zheming Cao, Siqi Lu, Pao Xu and Jun Qiang
Fishes 2022, 7(5), 299; https://doi.org/10.3390/fishes7050299 - 21 Oct 2022
Cited by 4 | Viewed by 2556
Abstract
Anti-Müllerian hormone (amh), an important regulator of gonad development in male teleosts, regulates the development and differentiation of germ cells. We performed transcriptional knock-down of amh in Nile tilapia (Oreochromis niloticus) using antisense RNA technology, resulting in down-regulation in [...] Read more.
Anti-Müllerian hormone (amh), an important regulator of gonad development in male teleosts, regulates the development and differentiation of germ cells. We performed transcriptional knock-down of amh in Nile tilapia (Oreochromis niloticus) using antisense RNA technology, resulting in down-regulation in the expression of amh transcription and Amh protein in males. Compared with the control groups, the fish in treatment groups with down-regulated amh had increased weight and an extremely significant decrease in the gonadosomatic index. Hematoxylin–eosin staining revealed impaired testis development and significant reductions in numbers of sperm. Serum estradiol levels were significantly increased, and the levels of testosterone, luteinizing hormone, and follicle-stimulating hormone were significantly decreased. RNA-sequencing analysis of the fish in the down-regulated amh and control groups identified 12,048 differentially expressed genes, of which 1281 were up-regulated and 10,767 were down-regulated. Kyoto Encyclopedia of Genes and Genomes analysis revealed that differentially expressed genes related to growth and development were mainly enriched in the Cell cycle, Endocytosis, TGF-beta signaling pathway, Wnt signaling pathway, FoxO signaling pathway, Insulin signaling pathway, and MAPK signaling pathway. The RNA-sequencing data accuracy was verified by qRT-PCR analysis of the expression levels of selected differentially expressed genes. The abnormal TGF-beta signaling pathway may cause fish weight gain, testis dysplasia, and abnormal spermatogenesis: smad5, smad3a, tgfb2, tgfbr1b, gsdf, and amh were significantly down-regulated. These findings indicated that antisense RNA technology has strong application prospects and can specifically knock down amh in Nile tilapia, resulting in an abnormal TGF-beta signaling pathway, inhibiting testis development and inducing weight gain. Full article
(This article belongs to the Special Issue Application of Biological Technologies in Aquatic Animal Genetic Breeding)
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