Biotechnological Strategies for Agro-Industrial Food Waste Management

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Industrial Fermentation".

Deadline for manuscript submissions: 30 June 2026 | Viewed by 3166

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Guest Editor
Department of Chemical, Biological, Pharmaceutical and Environmental Sciences, University of Messina, Messina, Italy
Interests: microbial fermentation; fermentation process management; biofuel; biorefinery; value-added products; microbial pigments production; microbial carotenoids; functional food
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Special Issue Information

Dear Colleagues,

Agro-industrial waste can be considered as the most abundant renewable resources on earth, expected to reach 3.40 billion metric tons by 2050. This waste, if disposed of untreated, can act as pollutants to the environment, affecting both humans and other living organisms. Their composition makes this waste suitable for their reuse to obtain added-value products, according to the Sustainable Development Goals (SDGs) of the United Nations, minimizing environmental problems and helping the economy and society. Currently agro-industrial waste is applied through biotechnological techniques as promising substrates for improving their stability and applicability in several sectors.

The aim of this Special Issue is to consider original studies on agro-industrial waste fermentation techniques through controlled fermentation processes carried out by selected microorganisms, for obtaining value-added products such as biofertilizers, enzymes, biopolymers, bioplastic, bioactive molecules, platform chemicals, food and feed supplements, single cell proteins, single cell oils, biofuel, bioenergy, and microbial pigments.

Dr. Alessia Tropea
Guest Editor

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Keywords

  • agricultural food waste
  • food waste management
  • bioenergy
  • biofuel
  • waste valorization
  • biotechnology
  • starter cultures
  • fermentation process

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Published Papers (2 papers)

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Research

16 pages, 2286 KB  
Article
Preliminary Screening of Non-Conventional Yeasts for Olive Mill Wastewater Valorization
by Gabriella Siesto, Rocchina Pietrafesa, Antonio Caporusso, Giorgia La Rocca, Grazia Alberico, Vito Valerio and Angela Capece
Fermentation 2026, 12(4), 188; https://doi.org/10.3390/fermentation12040188 - 8 Apr 2026
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Abstract
Olive mill wastewater (OMWW) is a highly polluting agro-industrial effluent characterized by elevated organic load, low pH, and high concentrations of phenolic compounds responsible for its phytotoxicity and dark coloration. In this study, 41 non-conventional yeast strains belonging to the University of Basilicata [...] Read more.
Olive mill wastewater (OMWW) is a highly polluting agro-industrial effluent characterized by elevated organic load, low pH, and high concentrations of phenolic compounds responsible for its phytotoxicity and dark coloration. In this study, 41 non-conventional yeast strains belonging to the University of Basilicata Yeast Collection (UBYC), were tested for both the oleaginous potential traits and OMWW detoxification capacity in comparison to two commercial oleaginous controls, Yarrowia lipolytica ATCC 46483 and Lipomyces tetrasporus Li-0407. Primary screening in synthetic medium under nitrogen-limited conditions revealed widespread intracellular lipid accumulation. Quantitative analysis showed lipid contents above 20% (w/w) in some strains, with Candida tropicalis AII122 (33.3%) and Pichia manshurica ML-3 (29.4%) exhibiting the highest values in synthetic medium. The cultivation of eight selected strains in synthetic medium supplemented with 15% (v/v) of OMWW reduced intracellular lipid accumulation, with the highest value of 6.48% for the 2R1 strain. Levels of phenol reduction and color removal were highly different among all the analyzed strains, and C. tropicalis AII122 achieved the highest phenolic reduction and decolorization ability. These findings demonstrate that indigenous non-conventional yeasts represent a source of natural biodiversity, supporting sustainable waste valorization strategies based on the use of selected microorganisms within a circular bioeconomy framework. Full article
(This article belongs to the Special Issue Biotechnological Strategies for Agro-Industrial Food Waste Management)
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24 pages, 1679 KB  
Article
Integrated Biorefinery of Brewer’s Spent Grain for Second-Generation Ethanol, Mycoprotein, and Bioactive Vinasse Production
by Sara Saldarriaga-Hernandez, José García-Béjar, Anahid Esparza-Vasquez, Rosa Leonor González-Díaz, Eduardo Joel López-Torres, Julio César López-Velázquez, Lorena Amaya-Delgado, Tomás García-Cayuela, Hemant Choudhary, Blake A. Simmons and Danay Carrillo-Nieves
Fermentation 2025, 11(11), 627; https://doi.org/10.3390/fermentation11110627 - 3 Nov 2025
Cited by 1 | Viewed by 2373
Abstract
Brewer’s spent grain (BSG), the main lignocellulosic by-product of the beer industry, represents an abundant yet underutilized resource with high potential for valorization. This study presents an integrated biorefinery approach to convert BSG into second-generation (2G) ethanol, bioactive vinasse for plant growth promotion, [...] Read more.
Brewer’s spent grain (BSG), the main lignocellulosic by-product of the beer industry, represents an abundant yet underutilized resource with high potential for valorization. This study presents an integrated biorefinery approach to convert BSG into second-generation (2G) ethanol, bioactive vinasse for plant growth promotion, and fungal biomass as a potential mycoprotein source. The biomass was first subjected to biological delignification using the white-rot fungus Ganoderma lucidum, after which two valorization routes were explored: (i) evaluation of the fungal biomass as a mycoprotein candidate and (ii) alcoholic fermentation for ethanol production. For the latter, three pretreatment strategies were assessed (diluted sulfuric acid and two deep eutectic solvents (DESs) based on choline chloride combined with either glycerol or lactic acid) followed by a one-pot enzymatic saccharification and fermentation using Kluyveromyces marxianus SLP1. The highest ethanol yield on substrate (YP/S) was achieved with [Ch]Cl:lactic acid pretreatment (0.46 g/g, 89.32% of theoretical). Vinasse, recovered after distillation, was characterized for organic acid content and tested on Solanum lycopersicum seed germination, showing promising biostimulant activity. Overall, this work highlights the potential of BSG as a sustainable feedstock within circular economy models, enabling the production of multiple bio-based products from a single residue. Full article
(This article belongs to the Special Issue Biotechnological Strategies for Agro-Industrial Food Waste Management)
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