Fermentation Technologies for the Production of High-Quality Feed

A special issue of Fermentation (ISSN 2311-5637). This special issue belongs to the section "Industrial Fermentation".

Deadline for manuscript submissions: closed (15 March 2025) | Viewed by 11744

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Guest Editor
Animal Nutrition Institute, Sichuan Agricultural University, Chengdu, China
Interests: animals; ruminant nutrition and feed science
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Special Issue Information

Dear Colleagues,

Fermentation has long been recognized as a valuable process for enhancing the nutritional quality and digestibility of feed ingredients. Fermented feed research focuses on enhancing animal feed quality and nutrition through fermentation. This involves using beneficial microorganisms to break down complex compounds into simpler forms, improving nutrient availability and digestion. Through fermentation techniques, such as solid-state or liquid fermentation, microorganism growth is optimized, resulting in several benefits. These include increased levels of enzymes and vitamins, decreased presence of anti-nutritional factors, and improved gut health. Additionally, alternative feed resources like by-products or food waste are explored to create highly nutritious feed.

The Special Issue aims to collect research and review papers related to the advancements and applications of fermentation technologies in the production of feed for animals.

Dr. Jianxin Xiao
Guest Editor

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Keywords

  • fermentation process
  • fermented feed
  • feed additives
  • feed resources
  • animal health
  • animal growth
  • nutritional value
  • feed preservation
  • microorganisms

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Related Special Issue

Published Papers (6 papers)

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Research

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20 pages, 2413 KiB  
Article
Effects of Replacing Rice Straw with Corn Straw Silage Treated with Different Additives on Growth Performance, Blood Parameters, and Rumen Microorganisms of Fattening Cattle
by Chunmei Zhang, Huawei Zou, Rui Hu, Quanhui Peng, Bai Xue, Lizhi Wang, Fali Wu, Yiping Tang, Zhisheng Wang and Jianxin Xiao
Fermentation 2025, 11(3), 107; https://doi.org/10.3390/fermentation11030107 - 20 Feb 2025
Viewed by 994
Abstract
This experiment aimed to evaluate the impact of various feed additive-treated silage corn straw on the growth performance, blood parameters, nutrient apparent digestibility, and rumen fermentation in Simmental cattle. Eighteen healthy Simmental bulls (275.64 ± 15.27 kg) were randomly assigned to three groups, [...] Read more.
This experiment aimed to evaluate the impact of various feed additive-treated silage corn straw on the growth performance, blood parameters, nutrient apparent digestibility, and rumen fermentation in Simmental cattle. Eighteen healthy Simmental bulls (275.64 ± 15.27 kg) were randomly assigned to three groups, each consisting of six bulls: a control group (CON) receiving a basal diet, an experimental group, (OS) wherein 20% of the rice straw in the control group’s diet was substituted with silage corn straw treated with organic acid, and another experimental group (MS) wherein 20% of the rice straw was replaced with silage corn straw mixed with corn flour, lactic acid bacteria, and organic acid. All cattle were fed at 08:30 and 16:30, twice each day, with unrestricted access to water throughout the study. The results indicated that silage had no effect on the growth and serum biochemical indexes of beef cattle among all groups. However, other parameters, mainly rumen fermentation parameters, nutrient digestibility, and rumen microorganisms, were affected by the silage. The MS group significantly increased the concentration of microbial protein (MCP) in the rumen of cattle compared to the CON group. The OS and MS groups had a similar apparent digestibility of ether extract and acid detergent fiber (ADF), but a higher digestibility of dry matter (DM) (p = 0.001) and crude protein (CP) (p < 0.001) compared to the CON group. The rumen bacterial community of the MS group had a lower abundance of Proteobacteria than the CON group (p = 0.016). The abundance of Firmicutes in the MS group was not significantly different from the CON group, but there was an increasing trend compared with the OS group (p = 0.054). A Spearman correlation analysis showed that the apparent digestibility of NDF and CP was negatively correlated with Succinivibrionaceae UCG-002 (r = −0.552, p = 0.018; r = −0.668, p = 0.002), Succinimonas (r = −0.774, p < 0.001; r = −0.513, p = 0.029), and Ruminobacter (r = −0.583, p = 0.011; r = −0.618, p = 0.006). The apparent digestibility of DM exhibited a negative correlation with Succinivibrionaceae UCG-002 (r = −0.538, p = 0.021) and Succinimonas (r = −0.642, p = 0.004). Overall, corn straw silage with mixed additives has more feeding value, which can improve rumen fermentation and regulate the rumen bacterial community. Straw silage can change the rumen microbial community structure to improve the apparent digestibility of nutrients. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
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19 pages, 1666 KiB  
Article
Intake of Condensed Tannins (Acacia mearnsii) by Lambs in Confinement and Its Impact on Growth Performance, Rumen Environment, and Meat
by Andrei L. R. Brunetto, Ana Luiza F. dos Santos, Isadora Zago, Guilherme L. Deolino, Luisa Nora, Vitor L. Molosse, Rafael V. P. Lago, Aline de C. Machado, Roger Wagner, Jelson N. Nauderer, Bianca F. Bissacotti, Aniela P. Kempka, Bruna Klein and Aleksandro S. Da Silva
Fermentation 2024, 10(12), 630; https://doi.org/10.3390/fermentation10120630 - 10 Dec 2024
Cited by 1 | Viewed by 1076
Abstract
This study determined whether adding condensed tannins to the diet of confined lamb improves performance, metabolism, health, and meat composition and quality. We used 24 Lacaune lambs with an average initial weight of 24.5 ± 0.24 kg. The animals were divided into 12 [...] Read more.
This study determined whether adding condensed tannins to the diet of confined lamb improves performance, metabolism, health, and meat composition and quality. We used 24 Lacaune lambs with an average initial weight of 24.5 ± 0.24 kg. The animals were divided into 12 pens with two animals each. In the treatment group, a black wattle extract was added to the lamb concentrate (2.5 g/kg dry matter), while the control group received only the basal diet (without extract). Black wattle contained 98.6 g/kg of condensed tannin. There was no difference between the performance variables, which included weight gain, feed intake, and feed efficiency. However, the group fed on condensed tannins showed higher carcass yield and a higher percentage of linked intramuscular fat. In meat, a lower proportion of saturated fatty acids combined with higher concentrations of unsaturated fatty acids was observed in lambs of the condensed tannin group. The polyunsaturated fatty acids (PUFA) were no different; however, a higher PUFA/SFA ratio was observed in animals with condensed tannin intake, and a higher proportion of ω6 and ω3 was observed in the meat of these animals. The meat’s protein and ash content did not differ between treatments, as did the meat’s water retention capacity, color, and luminosity. Furthermore, animals that fed on condensed tannins had lower cholesterol levels and higher globulin content. The values of erythrogram variables were lower in lambs that consumed condensed tannins. More significant ruminal bacterial activity was also observed in the group receiving condensed tannins. The antioxidant effect was verified with lower thiobarbituric acid reactive species (TBARS) levels and more significant antioxidant enzyme activity. We conclude that adding condensed tannins improves carcass yield and increases the percentage of fat in the carcass, improving the fatty acid profile, which may be linked to the antioxidant effect, in addition to the health benefits of the consumer of this product. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
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16 pages, 303 KiB  
Article
Combination of Essential Oils and Extracts Enriched with Tocopherols in Diet of Holstein Steers and Its Effect on Ruminal Fermentation, Antioxidant and Anti-Inflammatory Response, Feed Efficiency and Fatty Acid Profile in Meat
by João Gustavo Weschenfelder Wandscheer, Natalia Turcatto, Guilherme Luiz Deolindo, Mateus Henrique Signor, Alexandro Fritzen, Marcelo Vedovatto, Camila Ten Kathen Jung, Gilberto Vilmar Kozloski, Ana Carolina Hadlich Xavier, Roger Wagner, Bianca Fagan Bissacotti, Priscila Marquezan Copetti and Aleksandro Schafer da Silva
Fermentation 2024, 10(12), 616; https://doi.org/10.3390/fermentation10120616 - 1 Dec 2024
Viewed by 901
Abstract
Background: This experiment evaluated whether the combination of essential oils (thyme, rosemary, orange) with extracts of Cinnamomum and Quillaja saponaria (enriched with tocopherols) in steers’ diet has positive effects on the health, digestibility, growth, oxidative stress, and fatty acid profile of meat. Methods: [...] Read more.
Background: This experiment evaluated whether the combination of essential oils (thyme, rosemary, orange) with extracts of Cinnamomum and Quillaja saponaria (enriched with tocopherols) in steers’ diet has positive effects on the health, digestibility, growth, oxidative stress, and fatty acid profile of meat. Methods: The experiment included 24 Holstein steers, divided into two groups, randomly divided (treated = phytogenic mixture (n = 12); control = monensin (n = 12)), undergoing a growth phase of 120 days and 90 days of finishing in individual pens, with a monthly collection of blood, rumen fluid, feces, and feed. Results: In the finishing phase (days 150 to 210), there was a tendency (p = 0.07) toward more significant average daily weight gain when cattle consumed a phytogenic mixture than monensin. The use of the phytogenic product showed greater feed efficiency in the period from 150 to 240 days (p < 0.05), with higher levels of short-chain fatty acids (SCFAs), without differences in the composition of these SCFAs between the control and treated groups, with a reduction in apparent digestibility in the treated group. In blood parameters, the treated group showed higher erythrocyte counts (p < 0.05) without changes in leukocytes, lymphocytes, granulocytes, monocytes, and platelets. The phytogenic treatment demonstrated higher total protein and globulin levels, with increased glucose levels on days 30 and 210 (p < 0.05). Ceruloplasmin and haptoglobin levels increased in the treated group without changes in ferritin, transferrin, C-reactive protein, IgA, and heavy-chain immunoglobulins (p < 0.05). A reduction in oxidative stress was observed using the phytogenic agent, with a marked decrease in the levels of reactive oxygen species (ROS) and carbonyl proteins, with a tendency to increase glutathione S-transferase (p < 0.05). In meat, the phytogenic reduced saturated fatty acids with increased polyunsaturated fatty acids and increased the omega 6/omega 3 ratio. Lower levels of thiobarbituric acid reactive substances and ROS were detected in the meat combined with a higher concentration of total thiols in the treated group than in the control group. Conclusions: The use of the phytogenic in steers’ diets has shown to be a viable candidate for replacing monensin, presenting equal or superior performance results with systemic modulating effects on oxidative stress, meat quality, and serum and biochemical parameters that contribute to more substantial health and efficiency. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
31 pages, 2793 KiB  
Article
Impact of Inoculation with Pediococcus pentosaceus in Combination with Chitinase on Bale Core Temperature, Nutrient Composition, Microbial Ecology, and Ruminal Digestion of High-Moisture Alfalfa Hay
by Jayakrishnan Nair, Long Jin, Eric Chevaux, Tim A. McAllister and Yuxi Wang
Fermentation 2024, 10(10), 530; https://doi.org/10.3390/fermentation10100530 - 19 Oct 2024
Viewed by 811
Abstract
This study evaluated the effects of Pediococcus pentosaceus (PP) and chitinase combinations on the conservation and nutritive value of alfalfa high-moisture hay (HMH). P. pentosaceus [1012 colony forming unit/g fresh forage] combined with (g/tonne of fresh forage) 1.5 (PP + LC), 7.5 [...] Read more.
This study evaluated the effects of Pediococcus pentosaceus (PP) and chitinase combinations on the conservation and nutritive value of alfalfa high-moisture hay (HMH). P. pentosaceus [1012 colony forming unit/g fresh forage] combined with (g/tonne of fresh forage) 1.5 (PP + LC), 7.5 (PP + MC), or 15.0 (PP + HC) g of LANiHay01 chitinase (Exp. 1) or with LANiHay02 (PP + Fe; 1.5 g), LANiHay01 (PP + Pe; 1.5 g), or Sigma (PP + Si; 55 mg) chitinase/tonne (Exp. 2) were used in 2016. In 2017, PP was applied alone or in combination with LANiHay01 at 1.5 g (PP + LC) or 7.5 g (PP + MC) chitinase/tonne (Exp. 3 and 4). Deionized water and propionic acid (4.0 L/tonne of fresh forage in Exp. 1 and 2 and at 6.0 L/tonne of fresh forage in Exp. 3 and 4) were applied as neutral (CON) and positive control (CON+), respectively. The maximum temperature (r2 = 0.66) and NH3-N concentration (r2 = 0.80) of the HMH were positively related to total microbial populations. PP + MC had lower (p ≤ 0.05) yeast and mold counts than CON in Exp. 3 and 4 while the neutral detergent fiber degradability was greater (p < 0.01) for PP + MC and PP + LC than CON in Exp. 1 and 3, respectively. P. pentosaceus in combination with chitinase has the potential in conserving the nutrient quality of alfalfa HMH. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
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16 pages, 6013 KiB  
Article
The Effect of Covering Corn Silage with Tomato or Apple Pomace on Fermentation Parameters and Feed Quality
by Hayrullah Bora Ünlü, Önder Canbolat, Oktay Yerlikaya, Selim Esen, Valiollah Palangi and Maximilian Lackner
Fermentation 2024, 10(7), 372; https://doi.org/10.3390/fermentation10070372 - 20 Jul 2024
Viewed by 1763
Abstract
The current study assessed the effects of covering corn silage with tomato or apple pomace on fermentability and feed quality. The in vitro gas production test was performed using graded 100 mL syringes. Incubation times were 3, 6, 12, 24, 48, 72, and [...] Read more.
The current study assessed the effects of covering corn silage with tomato or apple pomace on fermentability and feed quality. The in vitro gas production test was performed using graded 100 mL syringes. Incubation times were 3, 6, 12, 24, 48, 72, and 96 h. In vitro gas generation characteristics were significantly altered by TP (tomato pomace) and AP (apple pomace), both alone and in conjunction with PE (polyethylene) films, regardless of their presence. As a result of the effects found on NH3-N concentration, aerobic stability, and yeast activity, TP and AP have the potential to become an eco-friendly alternative to PE films. The gas production from the immediately soluble fraction (a) of corn silage was only affected when the corn silage was covered with a combination of AP and PE compared to the CPE group (p < 0.001). The largest cluster includes correlations of the DOM-TDDM (r = 0.90), DOM-AA (r = 0.88), and Ash-TDDM (r = 0.86) correlations. The most substantial negative correlations were identified between DM-CO2 (r = −82), DM-Yeast (r = −0.79), and CF-DOM (r = −0.79). Nonetheless, the use of pomace as a silage cover presents an inexpensive alternative to plastic films for silage that does not have the environmental problems associated with persistent micro- and nanoplastics. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
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Review

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31 pages, 1252 KiB  
Review
Exploring Sustainable Aquafeed Alternatives with a Specific Focus on the Ensilaging Technology of Fish Waste
by Anastasiia Maksimenko, Leonid Belyi, Anna Podvolotskaya, Oksana Son and Liudmila Tekutyeva
Fermentation 2024, 10(5), 258; https://doi.org/10.3390/fermentation10050258 - 15 May 2024
Cited by 6 | Viewed by 5182
Abstract
The global increase in population has placed significant pressure on food security, leading to the emergence of aquaculture as a vital source of aquatic foods. However, rising costs and limited fish meal availability in aquafeeds have driven the search for alternative protein sources. [...] Read more.
The global increase in population has placed significant pressure on food security, leading to the emergence of aquaculture as a vital source of aquatic foods. However, rising costs and limited fish meal availability in aquafeeds have driven the search for alternative protein sources. While plant-based ingredients have been integrated into commercial aquafeeds, they come with challenges such as low protein content, palatability issues, and the presence of antinutritional factors. In this context, fish silage, made from fish waste and discarded fish, stands out as a promising alternative technology due to its cost-effectiveness and sustainability attributes. The production of fish silage involves the addition of organic/inorganic acids or lactic acid bacteria to homogenized fish waste, yielding a valuable mixture rich in peptides and free amino acids, offering significant nutritional benefits for animal diets. This review aims to promote sustainable practices in the aquaculture industry by analyzing research results related to ensiling technology, appraising the advantages and disadvantages of using fish silage as a feed ingredient, and focusing on emerging trends in this field. Full article
(This article belongs to the Special Issue Fermentation Technologies for the Production of High-Quality Feed)
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