Hematology: Diagnostic Techniques and Assays, 2nd Edition

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Clinical Laboratory Medicine".

Deadline for manuscript submissions: 31 October 2026 | Viewed by 517

Special Issue Editor


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Guest Editor
Department of Laboratory Medicine, College of Medicine, Korea University Ansan Hospital, Ansan, Republic of Korea
Interests: diagnostic hematology and immunology
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Hematology is the study of blood, its formation, function, and disorders. It is a vast and complex field that encompasses a wide range of topics, from the basic biology of blood cells to the diagnosis and treatment of blood diseases. In recent years, there have been significant advances in our understanding of hematology, which have led to the development of new diagnostic techniques and assays. These advances have had a profound impact on the care of patients with blood disorders, improving their quality of life and survival.

This 2nd Edition Special Issue of Diagnostics will focus on the latest advances in diagnostic techniques and assays in hematology. We will publish original research articles that describe new diagnostic techniques and assays, as well as clinical studies that evaluate the effectiveness of these techniques in the diagnosis and management of patients with blood disorders.

This Special Issue will cover a wide range of topics related to diagnostic techniques and assays in hematology, including the following:

  • New methods for the detection and characterization of blood cells;
  • Molecular diagnostics for hematological disorders;
  • Flow cytometry and immunophenotyping;
  • Cytogenetics and molecular cytogenetics;
  • Gene expression profiling and microarrays;
  • Next-generation sequencing;
  • Mass spectrometry;
  • Biomarkers and prognostication;
  • Point-of-care testing;
  • The application of diagnostic techniques to the study of hematopoietic stem cells;
  • The ethical and regulatory considerations of new diagnostic techniques.

Dr. Chihyun Cho
Guest Editor

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • diagnostic
  • hematology
  • technique
  • assay
  • biomarkers

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Published Papers (1 paper)

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Research

14 pages, 804 KB  
Article
Diagnostic Performance of Leukocyte Abnormality Detection in a Large Cohort of Healthy Blood Donors Using Sysmex XN Series Analyzers Integrated with Peripheral Blood Morphology and Flow Cytometry
by Francesca Romano, Valentina Becherucci, Sara Ciullini Mannurita, Edda Russo, Alessandra Mongia, Anna Maria Grazia Gelli, Alessandra Fanelli and Francesca Brugnolo
Diagnostics 2026, 16(5), 661; https://doi.org/10.3390/diagnostics16050661 - 25 Feb 2026
Viewed by 369
Abstract
Background: The Sysmex XN series (XN-1000 and XN-9100, Sysmex Corporation, Kobe, Japan) represents a latest-generation automated hematology platform integrating fluorescence-based technologies and multi-channel analysis (WDF and WPC) to improve leukocyte characterization. This study aimed to evaluate the performance of the Sysmex XN series [...] Read more.
Background: The Sysmex XN series (XN-1000 and XN-9100, Sysmex Corporation, Kobe, Japan) represents a latest-generation automated hematology platform integrating fluorescence-based technologies and multi-channel analysis (WDF and WPC) to improve leukocyte characterization. This study aimed to evaluate the performance of the Sysmex XN series in detecting leukocyte abnormalities flagged during routine complete blood count analysis in a large cohort of healthy donors, using morphological assessment and flow cytometry as confirmatory methods. Methods: Approximately 8000 healthy blood donors from the AOU Meyer Transfusion Centre were evaluated between 2021 and 2024. All samples underwent CBC analysis using the XN-1000 and XN-9100 analyzers with the WDF channel. Samples showing WBC-related flags were subjected to reflex testing with the WPC channel, followed by digital blood smear review using the DI-60 system (CellaVision, Lund, Sweden) and flow cytometric immunophenotyping. Results: WDF flags for “blasts/abnormal lymphocytes” were identified in 23 samples. Two samples were negative on WPC analysis as well as on morphological and flow cytometric evaluation. Among the remaining cases, WPC analysis identified flags for abnormal lymphocytes, atypical lymphocytes, or blasts, which were variably associated with reactive changes, transient immune activation, or clonal lymphoproliferative conditions. In one donor, monoclonal B-cell lymphocytosis was diagnosed by flow cytometry. Overall, reactive morphological features confirmed by flow cytometry were observed in approximately 50% of flagged cases. Conclusions: WPC analysis provides relevant additional diagnostic information and demonstrates higher specificity compared with the WDF channel alone; however, it does not fully resolve all instrument-generated flags, confirming the essential role of morphological assessment. Interestingly, the frequent occurrence of inflammatory profiles in recently vaccinated donors suggests that transient immune activation may influence leukocyte flagging. Larger studies are warranted to further investigate this association and to optimize the diagnostic performance of the WPC channel in donor screening. Full article
(This article belongs to the Special Issue Hematology: Diagnostic Techniques and Assays, 2nd Edition)
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