Laboratory Diagnosis of Infections

A special issue of Diagnostics (ISSN 2075-4418). This special issue belongs to the section "Clinical Laboratory Medicine".

Deadline for manuscript submissions: 31 August 2026 | Viewed by 1053

Special Issue Editor


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Guest Editor
Pediatric Intensive Care Unit, Hospital Sant Joan de Déu, University of Barcelona, Passeig de Sant Joan de Déu, 2, 08950 Barcelona, Spain
Interests: pediatrics; infectious diseases; critical care; biomarkers

Special Issue Information

Dear Colleagues,

We are pleased to announce this new Special Issue, entitled "Laboratory Diagnosis of Infections", in collaboration with Diagnostics, an open access journal published by MDPI, Switzerland.

The differential diagnosis of bacterial infectious diseases in relation to other infectious or inflammatory processes presents significant challenges. This complexity can result in the overuse of antibiotics, subsequently contributing to a rise in bacterial resistance. Furthermore, traditional cultures often yield low positivity rates and may require approximately 48 hours to provide results.

In response to these challenges, we have developed diagnostic panels that incorporate a variety of methodologies, including bacterial PCR in blood, microbiome analysis, and metabolomic profiling across different sample types. Additionally, we utilize biochemical markers for the detection of bacterial infections, such as white blood cell count (WBC), neutrophil percentage (NE%), platelet count (PLT), erythrocyte sedimentation rate, C-reactive protein, procalcitonin, pro-adrenomedullin, and specific interleukins, including IL-6 and IL-10.

We cordially invite you to contribute to this Special Issue by submitting articles that explore microbiological and biochemical markers associated with infectious diseases. We welcome the submission of a diverse range of contributions, including original research, experimental methodologies, reviews, and perspectives.

We look forward to receiving your valuable contributions.

Dr. Mònica Balaguer Gargallo
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Diagnostics is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2600 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • infections
  • laboratory diagnosis
  • biochemical markers
  • bacterial resistance

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Published Papers (2 papers)

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15 pages, 544 KB  
Article
Evaluation of the PowerChek™ Respiratory Virus Panel 1/2/3/4 for the Detection of 16 Respiratory Viruses: A Comparative Study with the Allplex™ Respiratory Panel Assay 1/2/3 and BioFire® Respiratory Panel 2.1 plus
by Hyeongyu Lee, Rokeya Akter, Jong-Han Lee and Sook Won Ryu
Diagnostics 2025, 15(21), 2713; https://doi.org/10.3390/diagnostics15212713 - 27 Oct 2025
Cited by 1 | Viewed by 664 | Correction
Abstract
Background/Objectives: Acute respiratory infections remain a major global public health concern affecting individuals across all ages. Accurate and rapid diagnosis of respiratory pathogens is crucial for effective patient management and infection control. Multiplex real-time polymerase chain reaction (PCR) assays have gained prominence [...] Read more.
Background/Objectives: Acute respiratory infections remain a major global public health concern affecting individuals across all ages. Accurate and rapid diagnosis of respiratory pathogens is crucial for effective patient management and infection control. Multiplex real-time polymerase chain reaction (PCR) assays have gained prominence over conventional methods for routine viral detection in clinical laboratories owing to their enhanced sensitivity and specificity; however, comparative performance data for PowerChek™ RVP remain limited. This study aimed to evaluate the diagnostic performance of the PowerChek™ Respiratory Virus Panel 1/2/3/4, which detects 16 respiratory viruses, including SARS-CoV-2, in nasopharyngeal swab (NPS) specimens. Methods: Overall, 336 NPS specimens were analyzed using the PowerChek™ RVP, BioFire® RP 2.1plus, and Allplex™ RP assays, with nucleic acid extraction performed using the Advansure™ E3 system. The performance metrics were calculated using two-by-two contingency tables. Results: Among 336 NPS specimens (232 positive, 104 negative), PowerChek™ RVP detected 226 positives with minimal discrepancies, showing high concordance with BioFire® RP 2.1plus (accuracy 94.6%, kappa 0.843–1.000). Fifteen discordant cases were identified in this study. Eleven could not be sequenced because of amplification failure and most had high Ct values (>30). Sequencing of four samples confirmed concordance with BioFire® RP 2.1plus and PowerChek™ RVP, whereas Allplex™ RP showed false-negative results. Conclusions: The PowerChek™ RVP assay demonstrated a high level of relative sensitivity, specificity, accuracy, diagnostic predictive values and strong concordance with comparable reference assays in identifying its targets. This assay is a reliable and efficient diagnostic tool for clinical laboratories to facilitate the accurate identification of respiratory pathogens. Full article
(This article belongs to the Special Issue Laboratory Diagnosis of Infections)
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Correction
Correction: Lee et al. Evaluation of the PowerChek™ Respiratory Virus Panel 1/2/3/4 for the Detection of 16 Respiratory Viruses: A Comparative Study with the Allplex™ Respiratory Panel Assay 1/2/3 and BioFire® Respiratory Panel 2.1 plus. Diagnostics 2025, 15, 2713
by Hyeongyu Lee, Rokeya Akter, Jong-Han Lee and Sook Won Ryu
Diagnostics 2025, 15(24), 3094; https://doi.org/10.3390/diagnostics15243094 - 5 Dec 2025
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In the original publication [...] Full article
(This article belongs to the Special Issue Laboratory Diagnosis of Infections)
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