Multicellular 3D Models of Cancer

A special issue of Cancers (ISSN 2072-6694). This special issue belongs to the section "Methods and Technologies Development".

Deadline for manuscript submissions: 15 December 2025 | Viewed by 761

Special Issue Editors


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Guest Editor
Division of Surgery and Interventional Science, University College London, London, UK
Interests: 3D in vitro cancer models; tumouroids; targeted treatments; theranostics; nanotechnology; precision oncology; patient-derived 3D models
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Co-Guest Editor
Centre for 3D Models of Health and Disease, University College London (UCL), Charles Bell House, London, UK
Interests: cancer models; 3D models; fibrosis; matrix stiffness; pancreatic cancer; liver cancer; ovarian cancer
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The focus of this Special Issue is to highlight novel research findings in multicellular 3D in vitro models of cancer. Multicellularity mimics the in vivo scenario and helps in developing relevant biomimetic models to investigate disease progression and test drugs. As highlighted by the FDA, 3D in vitro models hold great promise for low-cost animal-free testing.

We welcome research articles focused on developing complex models of the cancer microenvironment with a strong focus on multicellularity. We particularly encourage submissions on primary tissue work and the development of biomaterial-based animal-free models.

Prof. Dr. Marilena Loizidou
Prof. Dr. Eirini Velliou
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Cancers is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • 3D in vitro cancer models
  • multicellular cancer models
  • cancer microenvironment
  • tumour–stroma interactions

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Published Papers (1 paper)

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Research

22 pages, 58309 KiB  
Article
An Organoid Model for Translational Cancer Research Recapitulates Histoarchitecture and Molecular Hallmarks of Non-Small-Cell Lung Cancer
by Camilla T. Ekanger, Maria P. Ramnefjell, Maren S. F. Guttormsen, Joakim Hekland, Kristin Dahl-Michelsen, Maria L. Lotsberg, Ning Lu, Linda E. B. Stuhr, Laurence Hoareau, Pirjo-Riitta Salminen, Fabian Gärtner, Marianne Aanerud, Lars A. Akslen, James B. Lorens and Agnete S. T. Engelsen
Cancers 2025, 17(11), 1873; https://doi.org/10.3390/cancers17111873 - 3 Jun 2025
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Abstract
Background: Organoid cultures have received much attention in recent years due to the promise of patient-derived organoid cultures for exploration of personalized cancer treatment strategies. Organoid cultures have been established from a variety of malignancies; however, lack of a thorough histopathological analysis [...] Read more.
Background: Organoid cultures have received much attention in recent years due to the promise of patient-derived organoid cultures for exploration of personalized cancer treatment strategies. Organoid cultures have been established from a variety of malignancies; however, lack of a thorough histopathological analysis has limited the acceptance of organoid models as translational tools. Methods: Here, we aimed to establish patient-derived tumor-organoid (PDTO) models from human non-small-cell lung cancer (NSCLC) resection specimens and provide a thorough histopathological evaluation of the cultures. Results: We show that we were able to establish organoid cultures of lung adenocarcinomas (LUADs) and lung squamous cell carcinomas (LUSCs) successfully, and that the organoid cultures of different subtypes of NSCLC preserved the histoarchitecture and growth pattern of the tumors they derive from. Immunohistochemistry and AB-PAS staining confirmed the subtype-specific protein expression pattern and preserved mucin production in LUAD organoids. The genetic abnormalities of the tumors assessed by immunohistochemistry (IHC-P) were preserved in the organoid cultures. Conclusions: Our thorough study reveals conserved PDTO histopathology, supports further exploration, and encourages using PDTO models in translational research projects. PDTO models hold remarkable promise as patient-specific models and may be applied to predict therapy response in cases where molecular–pathological analyses pose significant management dilemmas, and they also may provide a platform for exploring the molecular mechanisms of therapy resistance in a biologically relevant model system. Full article
(This article belongs to the Special Issue Multicellular 3D Models of Cancer)
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