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Biosensors
Special Issues
Advanced Optical Biosensing: Design, Materials and Practical Applications
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Advanced Optical Biosensing: Design, Materials and Practical Applications

A Special Issue of Biosensors (ISSN 2079-6374) belonging to the section "Optical and Photonic Biosensors".

Deadline for manuscript submissions: 30 April 2027 | Viewed by 1016

Editors

Dr. Mimimorena Seggio

E-Mail Website
Guest Editor
Department of Engineering, Pegaso University, Naples, Italy
Interests: optical biosensors; optical chemical sensors; surface plasmon resonance (SPR); photochemistry; fluorescence; optical fibers
Dr. Rosalba Pitruzzella

E-Mail Website
Guest Editor Assistant
Department of Engineering, University of Campania Luigi Vanvitelli, Aversa, Italy
Interests: optical biosensors and optical-chemical sensors for environmental, food, biomedical, security, and biodefence applications

Special Issue Information

Dear Colleagues,

Recent advances in optical biosensing are reshaping analytical science by enabling rapid, label-free, sensitive, and potentially portable detection platforms for biomedical diagnostics, environmental monitoring, food safety, security, and biodefence. The integration of innovative optical transduction schemes, engineered materials, nanostructured interfaces, and robust molecular recognition strategies is opening new routes toward practical optical biosensors with improved selectivity, reproducibility, miniaturization, and field applicability. This Special Issue aims to collect high-quality original research articles, reviews, and short communications focused on the design, fabrication, characterization, and validation of advanced optical biosensing systems. Topics of interest include, but are not limited to, surface plasmon resonance sensors combined with molecular recognition elements (MREs), fiber-optic platforms for biosensing strategies, fluorescence-based biosensors, photonic and plasmonic nanostructures for sensing, molecular recognition layers (e.g., biological or biomimetic), biofunctionalization protocols, microfluidic integration, point-of-care devices, and data-assisted sensing approaches. Particular attention will be given to contributions that connect material/interface design with analytical performance and real-sample applications. Studies addressing biosensor reliability, selectivity, matrix effects, device portability, and translational potential are especially encouraged. Finally, innovative optical sensing principles that exploit new schemes, approaches, and materials are of interest to this Special Issue.

Dr. Mimimorena Seggio
Guest Editor

Dr. Rosalba Pitruzzella
Guest Editor Assistant

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 250 words) can be sent to the Editorial Office for assessment.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-anonymized peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • optical biosensors
  • optical-biomimetic sensors
  • optical fiber biosensors
  • spectroscopy
  • surface plasmon resonance (SPR)
  • plasmonic nanomaterials
  • molecular recognition layers
  • lab-on-fiber
  • point-of-care diagnostics
  • environmental, biomedical, and safety monitoring

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Published Papers (1 paper)

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Research

16 pages, 2189 KB  
Article
Biosensors Based on Plasmonic Spoon-Shaped Platforms as a Point-of-Care Tool for Escherichia coli Detection
by Francesco Arcadio, Alessandro Capo, Alessia Calabrese, Chiara Marzano, Mimimorena Seggio, Rosalba Pitruzzella, Federica Passeggio, Shahab Bashir, Muhammad Shoaib, Carla Zannella, Anna De Filippis, Giuseppe Portella, Luigi Zeni and Nunzio Cennamo
Biosensors 2026, 16(7), 371; https://doi.org/10.3390/bios16070371 - 8 Jul 2026
Viewed by 700
Abstract
The Enterobacteriaceae family is a significant source of foodborne pathogens and represents a severe threat to human and animal health. These bacteria can penetrate the dairy supply chain through direct contact with cattle and the livestock environment and can survive production processes. Escherichia [...] Read more.
The Enterobacteriaceae family is a significant source of foodborne pathogens and represents a severe threat to human and animal health. These bacteria can penetrate the dairy supply chain through direct contact with cattle and the livestock environment and can survive production processes. Escherichia coli (E. coli), one of the most diffuse bacteria in raw and processed milk, exposes consumers to the risk of contaminated milk. As a result of this exposition, several milk-borne illness outbreaks have been reported worldwide, underscoring the urgent need for effective detection and prevention measures. Conventional analysis methods are effective but have significant limitations, including the requirement of pre-treatment and pre-enrichment steps. Thus, the need for advanced detection techniques that can accurately identify these pathogens without pre-treatment steps is critical. In this work, a proof-of-concept biosensor based on a spoon-shaped optical biochip was developed to detect E. coli via surface plasmon resonance (SPR) phenomena and was combined with a polyclonal antibody layer against E. coli as a molecular recognition element (MRE). The proposed label-free biosensing strategy, achieved by exploiting simple SPR spoon-shaped biochips, exhibits a remarkable detection limit (6.8 colony-forming units, CFU/mL) and high specificity towards other interfering bacteria belonging to the Enterobacteriaceae family. In addition, tests on commercial milk samples were carried out, achieving recovery values of 95% and 102% for whole milk and infant milk, respectively. The proposed spoon-shaped biosensor enables label-free biosensing without the need for microfluidic systems. It provides a rapid response (10 min), paving the way for its use as a point-of-care test (POCT) in real-world settings. Full article
(This article belongs to the Special Issue Advanced Optical Biosensing: Design, Materials and Practical Applications)
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