New Trends in Electrochemical Biosensors for Healthcare and Environmental Monitoring

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Environmental Biosensors and Biosensing".

Deadline for manuscript submissions: closed (31 July 2025) | Viewed by 3638

Special Issue Editor


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Guest Editor
Center for Research in Environmental Chemical Engineering (CIQA), Córdoba Regional Faculty, National Technological University, Córdoba 5016, Argentina
Interests: (bio)sensors; electroanalysis; nanomaterials; materials synthesis; biomarkers detection; environmental chemistry

Special Issue Information

Dear Colleagues,

This Special Issue is focused on sharing recent advances in the design of electrochemical (bio)sensors for the quantification of biomarkers and analyses of environmental relevance. Since the first biosensor described by Clark and Lyons in the early 1960s, electrochemical biosensors have demonstrated themselves to be very competitive and powerful tools for a large number of applications due to their well-known advantages such as low cost, high sensitivity and selectivity, fast response, miniaturization, and portability. In this sense, it is important to remark on the key role of nano(bio)technology for the design of new schemes oriented to the preparation of the biorecognition layer and the electrochemical transduction of the analytical signal.

Original research articles, reviews, and prospective articles are welcome.

Dr. Pablo R. Dalmasso
Guest Editor

Manuscript Submission Information

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Keywords

  • biosensors
  • affinity biosensors
  • enzymatic biosensors
  • electrochemical sensing
  • nanomaterials
  • surface modification
  • biomarkers
  • emerging pollutants

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Published Papers (2 papers)

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Research

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12 pages, 1745 KB  
Article
Construction and Characterization of a Novel Direct Electron Transfer Type Enzymatic Sensor Using Spermidine Dehydrogenase
by Sheng Tong, Yuki Yaegashi, Mao Fukushi, Takumi Yanase, Junko Okuda-Shimazaki, Ryutaro Asano, Kazunori Ikebukuro, Madoka Nagata, Koji Sode and Wakako Tsugawa
Biosensors 2025, 15(10), 681; https://doi.org/10.3390/bios15100681 - 9 Oct 2025
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Abstract
This study reports on the direct electron transfer (DET) ability of the enzyme spermidine dehydrogenase (SpDH) and its use in a DET-type enzymatic sensor for detecting spermine. SpDH was found to exhibit internal electron transfer from its cofactor, flavin adenine dinucleotide (FAD), to [...] Read more.
This study reports on the direct electron transfer (DET) ability of the enzyme spermidine dehydrogenase (SpDH) and its use in a DET-type enzymatic sensor for detecting spermine. SpDH was found to exhibit internal electron transfer from its cofactor, flavin adenine dinucleotide (FAD), to heme b. This was confirmed by observing the heme b-derived reduction peak at 560 nm in the presence of spermine, the substrate. SpDH was immobilized on a gold electrode via a dithiobis (succinimidyl hexanoate) self-assembled monolayer. The cyclic voltammetry analysis of the SpDH-immobilized gold electrode revealed an increased oxidation current in the presence of 0.1 mM spermine with an onset potential of −0.14 V vs. Ag/AgCl in the absence of an additional external electron acceptor. This result confirmed that SpDH is capable of DET. Chronoamperometric analyses were conducted using an SpDH-immobilized gold electrode with spermine as the substrate under a 0 V oxidation potential vs. Ag/AgCl using an artificial saliva matrix containing 10 µM ascorbic acid and 100 µM uric acid. The sensor exhibited good linear correlation between the current increase and spermine concentration from 0.2 to 2.0 µM, with a limit of detection of 0.084 µM, which encompasses the physiologically relevant spermine concentration found in the saliva. Primary structure alignments and 3D structure predictions revealed that all SpDH homologs possess two conserved histidine residues in the same location on the surface as the heme b ligand of SpDH. This indicates their potential for DET-ability with an electrode. Full article
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Review

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58 pages, 8116 KB  
Review
Electrochemical Detection of Heavy Metals Using Graphene-Based Sensors: Advances, Meta-Analysis, Toxicity, and Sustainable Development Challenges
by Muhammad Saqib, Anna N. Solomonenko, Nirmal K. Hazra, Shojaa A. Aljasar, Elena I. Korotkova, Elena V. Dorozhko, Mrinal Vashisth and Pradip K. Kar
Biosensors 2025, 15(8), 505; https://doi.org/10.3390/bios15080505 - 4 Aug 2025
Cited by 2 | Viewed by 2889
Abstract
Contamination of food with heavy metals is an important factor leading to serious health concerns. Rapid identification of these heavy metals is of utmost priority. There are several methods to identify traces of heavy metals in food. Conventional methods for the detection of [...] Read more.
Contamination of food with heavy metals is an important factor leading to serious health concerns. Rapid identification of these heavy metals is of utmost priority. There are several methods to identify traces of heavy metals in food. Conventional methods for the detection of heavy metal residues have their limitations in terms of cost, analysis time, and complexity. In the last decade, voltammetric analysis has emerged as the most prominent electrochemical determination method for heavy metals. Voltammetry is a reliable, cost-effective, and rapid determination method. This review provides a detailed primer on recent advances in the development and application of graphene-based electrochemical sensors for heavy metal monitoring over the last decade. We critically examine aspects of graphene modification (fabrication process, stability, cost, reproducibility) and analytical properties (sensitivity, selectivity, rapid detection, lower detection, and matrix effects) of these sensors. Furthermore, to our knowledge, meta-analyses were performed for the first time for all investigated parameters, categorized based on graphene materials and heavy metal types. We also examined the pass–fail criteria according to the WHO drinking water guidelines. In addition, the effects of heavy metal toxicity on human health and the environment are discussed. Finally, the contribution of heavy metal contamination to the seventeen Sustainable Development Goals (SDGs) stated by the United Nations in 2015 is discussed in detail. The results confirm the significant impact of heavy metal contamination across twelve SDGs. This review critically examines the existing knowledge in this field and highlights significant research gaps and future opportunities. It is intended as a resource for researchers working on graphene-based electrochemical sensors for the detection of heavy metals in food safety, with the ultimate goal of improving consumer health protection. Full article
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