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Biosensors
Special Issues
CRISPR/Cas System-Based Biosensors
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CRISPR/Cas System-Based Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Nano- and Micro-Technologies in Biosensors".

Deadline for manuscript submissions: 31 December 2025 | Viewed by 4856

Special Issue Editors

Prof. Dr. Shiping Song

E-Mail Website
Guest Editor
1. Institute of Materiobiology, Shanghai University, Shanghai 201800, China
2. Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China
Interests: electrochemical biosensors; microfluidic biochips; biomedical diagnostics; food safety testing; environmental monitoring
Dr. Jing Su

E-Mail Website
Guest Editor
School of Perfume and Aroma Technology, Shanghai Institute of Technology, Shanghai 201418, China
Interests: synthesis of inorganic nanomaterials; DNA nanotechnology; biosensing in the early diagnosis of diseases

Special Issue Information

Dear Colleagues,

In recent years, the CRISPR/Cas system-based biosensors have attracted much attention in medical diagnoses, food safety, agricultural progress, and environmental monitoring. Furthermore, the CRISPR/Cas based analytic methods have evolved into versatile analytical platforms owing to their good sensitivity, high selectivity, and fast detection efficiency. A series of nucleic acid and non-nucleic acid detection biosensors based on the CRISPR platform have been developed. The high-performance CRISPR biosensors in combination with fluorescence, colorimetry, potentiometry, lateral flow assay have become the mainstream research trend. Newly discovered CRISPR/Cas systems and the engineering of the existing CRISPR/Cas systems would aid to increase the specificity, sensitivity, or stability of the diagnostic tools. To finish the clinical transition of diagnostics from their research use has also been the hot topic in recent years, and the CRISPR/Cas approaches will emerge as more promising and robust tools for various disease diagnoses and point-of-care tests in the future.

For this Special Issue, we welcome original research papers as well as reviews on the current developments in the design of high-sensitivity and reproducible systems with CRISPR/Cas-based biosensing technologies in the application of cancer and infectious diseases diagnoses. This includes the design of state-of-the-art CRISPR/Cas-based biosensing strategies. Theoretical research on the CRISPR/Cas-based biosensing mechanism is also encouraged. The design and development of lab-on-a-chip devices, wearable and plug-and-play biosensors, and portable/handheld CRISPR/Cas-based platforms for point-of-care applications are of special interest. Reviews should provide an in-depth examination of the most recent research in a specific context or discuss the existing and future obstacles for the commercial application of CRISPR detection technology and explore development opportunities and directions.

Prof. Dr. Shiping Song
Dr. Jing Su
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • CRISPR/Cas system
  • biosensor
  • biochip
  • lab on a chip
  • portable biosensing device
  • wearable biosensing device
  • POCT
  • disease diagnosis

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Published Papers (2 papers)

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Review

37 pages, 5820 KiB  
Review
Recent Advances in Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Proteins System-Based Biosensors
by Xianglin Xin, Jing Su, Haoran Cui, Lihua Wang and Shiping Song
Biosensors 2025, 15(3), 155; https://doi.org/10.3390/bios15030155 - 2 Mar 2025
Viewed by 852
Abstract
High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique enzymatic activities. They exhibit two key cleavage behaviors: [...] Read more.
High-sensitivity and high-specificity biodetection is critical for advancing applications in life sciences, biosafety, food safety, and environmental monitoring. CRISPR/Cas systems have emerged as transformative tools in biosensing due to their unparalleled specificity, programmability, and unique enzymatic activities. They exhibit two key cleavage behaviors: precise ON-target cleavage guided by specific protospacers, which ensures accurate target recognition, and bystander cleavage activity triggered upon target binding, which enables robust signal amplification. These properties make CRISPR/Cas systems highly versatile for designing biosensors for ultra-sensitive detection. This review comprehensively explores recent advancements in CRISPR/Cas system-based biosensors, highlighting their impact on improving biosensing performance. We discuss the integration of CRISPR/Cas systems with diverse signal readout mechanisms, including electrochemical, fluorescent, colorimetric, surface-enhanced Raman scattering (SERS), and so on. Additionally, we examine the development of integrated biosensing systems, such as microfluidic devices and portable biosensors, which leverage CRISPR/Cas technology for point-of-care testing (POCT) and high-throughput analysis. Furthermore, we identify unresolved challenges, aiming to inspire innovative solutions and accelerate the translation of these technologies into practical applications for diagnostics, food, and environment safety. Full article
(This article belongs to the Special Issue CRISPR/Cas System-Based Biosensors)
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21 pages, 3297 KiB  
Review
Harnessing CRISPR/Cas Systems for DNA and RNA Detection: Principles, Techniques, and Challenges
by Heyjin Son
Biosensors 2024, 14(10), 460; https://doi.org/10.3390/bios14100460 - 26 Sep 2024
Cited by 3 | Viewed by 3615
Abstract
The emergence of CRISPR/Cas systems has revolutionized the field of molecular diagnostics with their high specificity and sensitivity. This review provides a comprehensive overview of the principles and recent advancements in harnessing CRISPR/Cas systems for detecting DNA and RNA. Beginning with an exploration [...] Read more.
The emergence of CRISPR/Cas systems has revolutionized the field of molecular diagnostics with their high specificity and sensitivity. This review provides a comprehensive overview of the principles and recent advancements in harnessing CRISPR/Cas systems for detecting DNA and RNA. Beginning with an exploration of the molecular mechanisms of key Cas proteins underpinning CRISPR/Cas systems, the review navigates the detection of both pathogenic and non-pathogenic nucleic acids, emphasizing the pivotal role of CRISPR in identifying diverse genetic materials. The discussion extends to the integration of CRISPR/Cas systems with various signal-readout techniques, including fluorescence, electrochemical, and colorimetric, as well as imaging and biosensing methods, highlighting their advantages and limitations in practical applications. Furthermore, a critical analysis of challenges in the field, such as target amplification, multiplexing, and quantitative detection, underscores areas requiring further refinement. Finally, the review concludes with insights into the future directions of CRISPR-based nucleic acid detection, emphasizing the potential of these systems to continue driving innovation in diagnostics, with broad implications for research, clinical practice, and biotechnology. Full article
(This article belongs to the Special Issue CRISPR/Cas System-Based Biosensors)
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