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Biosensors
Special Issues
Micro/Nanofluidic System-Based Biosensors
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Micro/Nanofluidic System-Based Biosensors

A special issue of Biosensors (ISSN 2079-6374). This special issue belongs to the section "Biosensor Materials".

Deadline for manuscript submissions: 25 November 2025 | Viewed by 2241

Special Issue Editors

Dr. Mengren Wu

E-Mail Website
Guest Editor
Department of Mechanical Engineering, Stanford University, Palo Alto, CA, USA
Interests: electrokinetic phenomena; biosensors; microfluidics; lab on a chip; acoustofluidics
Dr. Yuan Gao

E-Mail Website
Guest Editor
Department of Mechanical Engineering, University of Memphis, Memphis, TN 38152, USA
Interests: microfluidics; Lab on a Chip; acoustofluidics; bubble; biomedical applications
Special Issues, Collections and Topics in MDPI journals
Dr. Yang Lin

E-Mail Website
Guest Editor
Department of Mechanical, Industrial, and Systems Engineering, University of Rhode Island, Kingston, RI 02881, USA
Interests: microfluidics; acoustofluidics; organ-on-a-chip; environmental monitoring; biosensing
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

Miniaturized biosensors, leveraging advanced micro- and nanofabrication techniques, have led to significant advancements in various fields. These devices enable precise manipulation of microenvironments, reduce reagent consumption, and offer high-throughput performance. Their integration with automation systems enhances efficiency and reproducibility, while increased sensitivity allows for the detection of low-abundance analytes, making them invaluable in applications such as point-of-care diagnostics and environmental monitoring.

This Special Issue, titled “Micro/Nanofluidic System-Based Biosensors”, aims to accelerate the advancement of biosensing technologies by inviting research papers, short communications, and review articles that focus on the following:

  • Design and development of advanced biosensors for detecting biomarkers, pathogens, or environmental pollutants in micro/nanofluidic systems.
  • Innovations enhancing the sensitivity, precision, functionality and throughput of micro/nanofluidic system-based biosensing
  • Single-cell/organoid analysis techniques for exploring cellular heterogeneity at functional and molecular levels.
  • Approaches to cost-effective micromanufacturing, automation, and efficient reagent usage in micro/nanofluidic systems.

Dr. Mengren Wu
Dr. Yuan Gao
Dr. Yang Lin
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Biosensors is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2200 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microfluidics
  • nanofluidics
  • biomarkers
  • pathogens
  • pollutants
  • single cell

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Further information on MDPI's Special Issue policies can be found here.

Published Papers (2 papers)

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Research

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21 pages, 3587 KiB  
Article
Carboxymethyl Dextran-Based Biosensor for Simultaneous Determination of IDO-1 and IFN-Gamma in Biological Material
by Zuzanna Zielinska, Anna Sankiewicz, Natalia Kalinowska, Beata Zelazowska-Rutkowska, Tomasz Guszcz, Leszek Ambroziak, Miroslaw Kondratiuk and Ewa Gorodkiewicz
Biosensors 2025, 15(7), 444; https://doi.org/10.3390/bios15070444 - 10 Jul 2025
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Abstract
Indoleamine 2,3-dioxygenase 1 (IDO-1) and interferon-gamma (IFN-γ) are proteins that play a significant role in inflammatory conditions and tumor development. The detection of IDO1 and IFN-γ is crucial for understanding their interplay in immune responses. This study introduced a novel method for the [...] Read more.
Indoleamine 2,3-dioxygenase 1 (IDO-1) and interferon-gamma (IFN-γ) are proteins that play a significant role in inflammatory conditions and tumor development. The detection of IDO1 and IFN-γ is crucial for understanding their interplay in immune responses. This study introduced a novel method for the simultaneous quantitative determination of IDO-1 and IFN-γ in different biological samples/materials. The method is based on an optical biosensor, with surface plasmon resonance detection carried out by the imaging version of the sensor (SPRi). Biotinylated antibodies immobilized on the surfaces of the linker and carboxymethylated dextran served as the recognition elements for the developed biosensor. Relevant studies were conducted to optimize the activities of the biosensor by employing appropriate reagent concentrations. Validation was performed for each protein separately; low detection and quantification limits were obtained (for IDO-1 LOD = 0.27 ng/mL, LOQ = 0.81 ng/mL; for IFN-γ LOD = 1.76 pg/mL and LOQ = 5.29 pg/mL). The sensor operating ranges were 0.001–10 ng/mL for IDO-1 and 0.1–1000 pg/mL for IFN-γ. The constructed biosensor demonstrated its sensitivity and precision when the appropriate analytical parameters were determined, based on the proposed method. It can also selectively capture IDO-1 and IFN-γ from a large sample matrix. The biosensor efficiency was confirmed by the determination of IDO-1 and IFN-γ in simultaneous measurements of the plasma and urine samples of patients diagnosed with bladder cancer and the control group. The outcomes were compared to those obtained using a certified ELISA test, demonstrating convergence between the two methodologies. The preliminary findings demonstrate the biosensor’s efficacy and suitability for comprehensive analyses of the examined biological samples. Full article
(This article belongs to the Special Issue Micro/Nanofluidic System-Based Biosensors)
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Review

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40 pages, 3743 KiB  
Review
Droplet Generation and Manipulation in Microfluidics: A Comprehensive Overview of Passive and Active Strategies
by Andrea Fergola, Alberto Ballesio, Francesca Frascella, Lucia Napione, Matteo Cocuzza and Simone Luigi Marasso
Biosensors 2025, 15(6), 345; https://doi.org/10.3390/bios15060345 - 29 May 2025
Viewed by 1862
Abstract
Droplet-based microfluidics (DBM) has emerged as a powerful tool for a wide range of biochemical applications, from single-cell analysis and drug screening to diagnostics and tissue engineering. This review provides a comprehensive overview of the latest advancements in droplet generation and trapping techniques, [...] Read more.
Droplet-based microfluidics (DBM) has emerged as a powerful tool for a wide range of biochemical applications, from single-cell analysis and drug screening to diagnostics and tissue engineering. This review provides a comprehensive overview of the latest advancements in droplet generation and trapping techniques, highlighting both passive and active approaches. Passive methods—such as co-flow, cross-flow, and flow-focusing geometries—rely on hydrodynamic instabilities and capillary effects, offering simplicity and integration with compact devices, though often at the cost of tunability. In contrast, active methods exploit external fields—electric, magnetic, thermal, or mechanical—to enable on-demand droplet control, allowing for higher precision and throughput. Furthermore, we explore innovative trapping mechanisms such as hydrodynamic resistance networks, microfabricated U-shaped wells, and anchor-based systems that enable precise spatial immobilization of droplets. In the final section, we also examine active droplet sorting strategies, including electric, magnetic, acoustic, and thermal methods, as essential tools for downstream analysis and high-throughput workflows. These manipulation strategies facilitate in situ chemical and biological analyses, enhance experimental reproducibility, and are increasingly adaptable to industrial-scale applications. Emphasis is placed on the design flexibility, scalability, and biological compatibility of each method, offering critical insights for selecting appropriate techniques based on experimental needs and operational constraints. Full article
(This article belongs to the Special Issue Micro/Nanofluidic System-Based Biosensors)
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