Micro- and Nano-Technologies for Cell Analysis

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Nanobiotechnology and Biofabrication".

Deadline for manuscript submissions: closed (31 May 2025) | Viewed by 2286

Special Issue Editors


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Guest Editor
1. Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, Kobe 6500047, Hyogo, Japan
2. Department of Medical Device Engineering, Graduate School of Medicine, Kobe University, Kobe 6500017, Hyogo, Japan
3. Health and Medical Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 7610395, Kagawa, Japan
Interests: nano/micro system; lab on a chip; MicroTAS; BioMEMS; microfabrication

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Guest Editor
Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu 761-0395, Kagawa, Japan
Interests: cell chip; single cell analysis; biochip; biosensor; cancer
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Special Issue Information

Dear Colleagues,

Micro- and nano-technologies have become essential components for a better analysis of single cells, cellular tissues and organs in the human body. Recent advances in micro- and nano-technologies such as cell chips, Lab on a Chip, microphysiological system and BioMEMS have made major contributions to improving the current biological technologies such as liquid biopsy, point of care, drug development, regenerative medicine, etc.

This Special Issue, will therefore focus on original research papers and comprehensive reviews dealing with cutting-edge micro- and nano-technologies for cell or cell-relative molecule analysis. The topics of interest for this Special Issue include, but are not limited to, the following:

  1. Advanced micro- or nano-fabrication methods for biological experiments or surgery;
  1. Advanced biosensors for analyzing cells (cell-relative molecules) or cellular tissues;
  1. Advanced imaging method for observing and evaluating single-cell or tissue conditions;
  1. Advanced microfluidics device or technology for cell analysis;
  1. Advanced micro/nano-actuators or robotics for cell analysis and manipulations.

Other relevant technical articles and state-of-the-art technology reviews in the field are also welcome.

We look forward to receiving your contributions.

Dr. Hidetaka Ueno
Dr. Shohei Yamamura
Guest Editors

Manuscript Submission Information

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Keywords

  • cell-based assay
  • high-throughput screening
  • diagnosis
  • tissue engineering
  • micro- and nano-technology
  • cell chip
  • lab on a chip
  • BioMEMS
  • 3D-printed device
  • biosensor

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Published Papers (2 papers)

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Research

20 pages, 5322 KB  
Article
Regulation of Tetraspanin CD63 in Chronic Myeloid Leukemia (CML): Single-Cell Analysis of Asymmetric Hematopoietic Stem Cell Division Genes
by Christophe Desterke, Annelise Bennaceur-Griscelli and Ali G. Turhan
Bioengineering 2025, 12(8), 830; https://doi.org/10.3390/bioengineering12080830 - 31 Jul 2025
Viewed by 527
Abstract
(1) Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder driven by the BCR::ABL oncoprotein. During the chronic phase, Philadelphia chromosome-positive hematopoietic stem cells generate proliferative myeloid cells with various stages of maturation. Despite this expansion, leukemic stem cells (LSCs) retain self-renewal capacity [...] Read more.
(1) Background: Chronic myeloid leukemia (CML) is a myeloproliferative disorder driven by the BCR::ABL oncoprotein. During the chronic phase, Philadelphia chromosome-positive hematopoietic stem cells generate proliferative myeloid cells with various stages of maturation. Despite this expansion, leukemic stem cells (LSCs) retain self-renewal capacity via asymmetric cell divisions, sustaining the stem cell pool. Quiescent LSCs are known to be resistant to tyrosine kinase inhibitors (TKIs), potentially through BCR::ABL-independent signaling pathways. We hypothesize that dysregulation of genes governing asymmetric division in LSCs contributes to disease progression, and that their expression pattern may serve as a prognostic marker during the chronic phase of CML. (2) Methods: Genes related to asymmetric cell division in the context of hematopoietic stem cells were extracted from the PubMed database with the keyword “asymmetric hematopoietic stem cell”. The collected relative gene set was tested on two independent bulk transcriptome cohorts and the results were confirmed by single-cell RNA sequencing. (3) Results: The expression of genes involved in asymmetric hematopoietic stem cell division was found to discriminate disease phases during CML progression in the two independent transcriptome cohorts. Concordance between cohorts was observed on asymmetric molecules downregulated during blast crisis (BC) as compared to the chronic phase (CP). This downregulation during the BC phase was confirmed at single-cell level for SELL, CD63, NUMB, HK2, and LAMP2 genes. Single-cell analysis during the CP found that CD63 is associated with a poor prognosis phenotype, with the opposite prediction revealed by HK2 and NUMB expression. The single-cell trajectory reconstitution analysis in CP samples showed CD63 regulation highlighting a trajectory cluster implicating HSPB1, PIM2, ANXA5, LAMTOR1, CFL1, CD52, RAD52, MEIS1, and PDIA3, known to be implicated in hematopoietic malignancies. (4) Conclusion: Regulation of CD63, a tetraspanin involved in the asymmetric division of hematopoietic stem cells, was found to be associated with poor prognosis during CML progression and could be a potential new therapeutic target. Full article
(This article belongs to the Special Issue Micro- and Nano-Technologies for Cell Analysis)
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17 pages, 3137 KB  
Article
Microdifferential Pressure Measurement Device for Cellular Microenvironments
by Mami Akaike, Jun Hatakeyama, Yoichi Saito, Yoshitaka Nakanishi, Kenji Shimamura and Yuta Nakashima
Bioengineering 2025, 12(1), 3; https://doi.org/10.3390/bioengineering12010003 - 24 Dec 2024
Cited by 1 | Viewed by 1195
Abstract
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The [...] Read more.
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The device comprises a glass substrate and a microchannel constructed of polydimethylsiloxane, polytetrafluoroethylene tubes, a glass capillary, and a microsyringe pump. This device obviates the need for electrical measurements, relying solely on the displacement of ultrapure water within the microchannel to assess the micropressure in embryos. First, the device was subjected to arbitrary pressures, and the relationship between the pressure and the displacement of ultrapure water in the microchannel was determined. Calibration results showed that the displacement dx [μm] could be calculated from the pressure P [Pa] using the equation dx = 0.36 P. The coefficient of determination was shown to be 0.87, indicating a linear response. When utilized to measure brain ventricular pressure in mouse embryos, the fabricated device yielded an average pressure reading of 1313 ± 640 Pa. This device can facilitate the measurement of pressure within microcavities in living tissues and other areas requiring precise and localized pressure evaluations. Full article
(This article belongs to the Special Issue Micro- and Nano-Technologies for Cell Analysis)
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