Micro- and Nano-Technologies for Cell Analysis

A special issue of Bioengineering (ISSN 2306-5354). This special issue belongs to the section "Nanobiotechnology and Biofabrication".

Deadline for manuscript submissions: 31 May 2025 | Viewed by 1301

Special Issue Editors


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Guest Editor
1. Center for Advanced Medical Engineering Research & Development (CAMED), Kobe University, Kobe 6500047, Hyogo, Japan
2. Department of Medical Device Engineering, Graduate School of Medicine, Kobe University, Kobe 6500017, Hyogo, Japan
3. Health and Medical Research Institute, Department of Life Science and Biotechnology, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 7610395, Kagawa, Japan
Interests: nano/micro system; lab on a chip; MicroTAS; BioMEMS; microfabrication

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Guest Editor
Health and Medical Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), 2217-14 Hayashi-cho, Takamatsu 761-0395, Kagawa, Japan
Interests: cell chip; single cell analysis; biochip; biosensor; cancer
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Special Issue Information

Dear Colleagues,

Micro- and nano-technologies have become essential components for a better analysis of single cells, cellular tissues and organs in the human body. Recent advances in micro- and nano-technologies such as cell chips, Lab on a Chip, microphysiological system and BioMEMS have made major contributions to improving the current biological technologies such as liquid biopsy, point of care, drug development, regenerative medicine, etc.

This Special Issue, will therefore focus on original research papers and comprehensive reviews dealing with cutting-edge micro- and nano-technologies for cell or cell-relative molecule analysis. The topics of interest for this Special Issue include, but are not limited to, the following:

  1. Advanced micro- or nano-fabrication methods for biological experiments or surgery;
  1. Advanced biosensors for analyzing cells (cell-relative molecules) or cellular tissues;
  1. Advanced imaging method for observing and evaluating single-cell or tissue conditions;
  1. Advanced microfluidics device or technology for cell analysis;
  1. Advanced micro/nano-actuators or robotics for cell analysis and manipulations.

Other relevant technical articles and state-of-the-art technology reviews in the field are also welcome.

We look forward to receiving your contributions.

Dr. Hidetaka Ueno
Dr. Shohei Yamamura
Guest Editors

Manuscript Submission Information

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Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Bioengineering is an international peer-reviewed open access monthly journal published by MDPI.

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Keywords

  • cell-based assay
  • high-throughput screening
  • diagnosis
  • tissue engineering
  • micro- and nano-technology
  • cell chip
  • lab on a chip
  • BioMEMS
  • 3D-printed device
  • biosensor

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Published Papers (1 paper)

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Research

17 pages, 3137 KiB  
Article
Microdifferential Pressure Measurement Device for Cellular Microenvironments
by Mami Akaike, Jun Hatakeyama, Yoichi Saito, Yoshitaka Nakanishi, Kenji Shimamura and Yuta Nakashima
Bioengineering 2025, 12(1), 3; https://doi.org/10.3390/bioengineering12010003 - 24 Dec 2024
Viewed by 838
Abstract
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The [...] Read more.
Mechanical forces influence cellular proliferation, differentiation, tissue morphogenesis, and functional expression within the body. To comprehend the impact of these forces on living organisms, their quantification is essential. This study introduces a novel microdifferential pressure measurement device tailored for cellular-scale pressure assessments. The device comprises a glass substrate and a microchannel constructed of polydimethylsiloxane, polytetrafluoroethylene tubes, a glass capillary, and a microsyringe pump. This device obviates the need for electrical measurements, relying solely on the displacement of ultrapure water within the microchannel to assess the micropressure in embryos. First, the device was subjected to arbitrary pressures, and the relationship between the pressure and the displacement of ultrapure water in the microchannel was determined. Calibration results showed that the displacement dx [μm] could be calculated from the pressure P [Pa] using the equation dx = 0.36 P. The coefficient of determination was shown to be 0.87, indicating a linear response. When utilized to measure brain ventricular pressure in mouse embryos, the fabricated device yielded an average pressure reading of 1313 ± 640 Pa. This device can facilitate the measurement of pressure within microcavities in living tissues and other areas requiring precise and localized pressure evaluations. Full article
(This article belongs to the Special Issue Micro- and Nano-Technologies for Cell Analysis)
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