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Applied Sciences
Special Issues
3D Bioprinted Tissues for Personalized Medicine Approaches
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3D Bioprinted Tissues for Personalized Medicine Approaches

A special issue of Applied Sciences (ISSN 2076-3417). This special issue belongs to the section "Additive Manufacturing Technologies".

Deadline for manuscript submissions: closed (20 March 2023) | Viewed by 8399

Special Issue Editors

Dr. Livia Roseti

E-Mail Website
Guest Editor
Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy
Interests: translational research; 3D printing; quality systems (GMP, GLP, ISO); risk analysis; tissue engineering; regenerative medicine; biomaterials
Dr. Mauro Petretta

E-Mail Website
Guest Editor
Laboratorio RAMSES, IRCCS Istituto Ortopedico Rizzoli, Via di Barbiano 1/10, 40136 Bologna, Italy
Interests: biofabrication; bioprinting; 3D printing; biomaterials; bioengineering; prosthetic systems and artificial organs; tissue engineering and regenerative medicine; tissue models; drug discovery; personalized medicine; multifunctional scaffolds; reverse engineering; additive manufacturing

Special Issue Information

Dear Colleagues,

Three-dimensional bioprinting is an Additive Manufacturing approach that represents a groundbreaking innovation in regenerative medicine. The computer-guided printing of viable cells, biomaterials, and bioactive stimuli enables accurate spatial localization, and punctual control over architecture, cell distribution, and chemical composition. In this way, the resulting bioengineered structures effectively mimic the structural and functional characteristics of native tissues/organs. The use of 3D starting models obtained by patient medical images guarantees a match between implant and defect size, thereby allowing for the development of customizable structures depending on the patient’s clinical need. Recently, 3D bioprinting has been also integrated with techniques such as 3D cell culture, bioreactor technology, microfluidics, and organ-on-a-chip in order to improve outcomes and enable new applications in personalized medicine.

Despite encouraging results, bioprinting is still in its early phase and has several bridges to cross before meeting the current clinical and industrial needs. Efforts are mostly concentrated on the development and optimization of the bioink features and of the manufacturing process.

This Special Issue will provide new insights on the potential offered by the bioprinting technique, by presenting scientific research updates in different fields of applications, with the vision of moving towards clinical or industrial translation. We invite authors to propose original studies, review articles, and communications introducing advancements in biofabrication of novel tissues and organ substitutes or models, or innovative applications in personalized medicine, pharmaceutics, or biosensors achieved through a bioprinting/biofabrication approach.

Potential topics include but are not limited to the use of bioprinting to produce:

  • Novel bioink formulations development for research and clinical applications;
  • Engineered tissues and organs for regenerative medicine;
  • In situ bioprinting;
  • Tissues, organs, or disease models for drug screening and discovery applications;
  • Tissue-on-chip, organ-on-chip, disease-on-chip diagnostic models.

Dr. Livia Roseti
Dr. Mauro Petretta
Guest Editors

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Applied Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2400 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • 3D bioprinting
  • biofabrication
  • tissue engineering
  • regenerative medicine
  • bioink
  • cell printing
  • spheroids printing
  • organoids
  • personalized medicine
  • tissue models
  • custom-made tissues and organs
  • organ-on-chip
  • drug discovery

Published Papers (4 papers)

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Research

11 pages, 5404 KiB  
Article
Design and Implementation of an Accessible 3D Bioprinter: Benchmarking the Performance of a Home-Made Bioprinter against a Professional Bioprinter
by Paolo D’Atanasio, Noemi Fiaschini, Antonio Rinaldi, Alessandro Zambotti, Lorenzo Cantini, Mariateresa Mancuso and Francesca Antonelli
Appl. Sci. 2023, 13(18), 10213; https://doi.org/10.3390/app131810213 - 11 Sep 2023
Viewed by 991
Abstract
The tremendous application potential of 3D bioprinting in the biomedical field is witnessed by the ever-increasing interest in this technology over the past few years. In particular, the possibility of obtaining 3D cellular models that mimic tissues with precision and reproducibility represents a [...] Read more.
The tremendous application potential of 3D bioprinting in the biomedical field is witnessed by the ever-increasing interest in this technology over the past few years. In particular, the possibility of obtaining 3D cellular models that mimic tissues with precision and reproducibility represents a definitive advance for in vitro studies dealing with the biological mechanisms of cell growth, death and proliferation and is at the basis of the responses of healthy and pathological tissues to drugs and therapies. However, the impact of 3D bioprinting on research is limited by the high costs of professional 3D bioprinters, which represent an obstacle to the widespread access and usability of this technology. In this work, we present a 3D bioprinter that was developed in-house by modifying a low-cost commercial 3D printer by replacing the default extruder used to print plastic filaments with a custom-made syringe extruder that is suitable for printing bioinks. The modifications made to the 3D printer include adjusting the size of the extruder to accommodate a 1 mL syringe and reducing the extruder’s size above the printer. To validate the performance of the home-made bioprinter, some main printing characteristics, the cell vitality and the possibility of bioprinting CAD-designed constructs were benchmarked against a renowned professional 3D bioprinter by RegenHu. According to our findings, our in-house 3D bioprinter was mostly successful in printing a complex glioblastoma tumor model with good performances, and it managed to maintain a cell viability that was comparable to that achieved by a professional bioprinter. This suggests that an accessible open-source 3D bioprinter could be a viable option for research and development (R&D) laboratories interested in pre-commercial 3D bioprinting advancements. Full article
(This article belongs to the Special Issue 3D Bioprinted Tissues for Personalized Medicine Approaches)
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13 pages, 3113 KiB  
Article
In Vitro Synovial Membrane 3D Model Developed by Volumetric Extrusion Bioprinting
by Mauro Petretta, Simona Villata, Marika Pia Scozzaro, Livia Roseti, Marta Favero, Lucia Napione, Francesca Frascella, Candido Fabrizio Pirri, Brunella Grigolo and Eleonora Olivotto
Appl. Sci. 2023, 13(3), 1889; https://doi.org/10.3390/app13031889 - 01 Feb 2023
Cited by 2 | Viewed by 1734
Abstract
(1) Background: Synovial tissue plays a fundamental role in inflammatory processes. Therefore, understanding the mechanisms regulating healthy and diseased synovium functions, as in rheumatic diseases, is crucial to discovering more effective therapies to minimize or prevent pathological progress. The present study aimed at [...] Read more.
(1) Background: Synovial tissue plays a fundamental role in inflammatory processes. Therefore, understanding the mechanisms regulating healthy and diseased synovium functions, as in rheumatic diseases, is crucial to discovering more effective therapies to minimize or prevent pathological progress. The present study aimed at developing a bioartificial synovial tissue as an in vitro model for drug screening or personalized medicine applications using 3D bioprinting technology. (2) Methods: The volumetric extrusion technique has been used to fabricate cell-laden scaffolds. Gelatin Methacryloyl (GelMA), widely applied in regenerative medicine and tissue engineering, was selected as a bioink and combined with an immortalized cell line of fibroblast-like synoviocytes (K4IM). (3) Results: Three different GelMA formulations, 7.5–10–12.5% w/v, were tested for the fabrication of the scaffold with the desired morphology and internal architecture. GelMA 10% w/v was chosen and combined with K4IM cells to fabricate scaffolds that showed high cell viability and negligible cytotoxicity for up to 14 days tested by Live & Dead and lactate dehydrogenase assays. (4) Conclusions: We successfully 3D bioprinted synoviocytes-laden scaffolds as a proof-of-concept (PoC) towards the fabrication of a 3D synovial membrane model suitable for in vitro studies. However, further research is needed to reproduce the complexity of the synovial microenvironment to better mimic the physiological condition. Full article
(This article belongs to the Special Issue 3D Bioprinted Tissues for Personalized Medicine Approaches)
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13 pages, 2503 KiB  
Article
3D Printing of Human Ossicle Models for the Biofabrication of Personalized Middle Ear Prostheses
by Jacob Dairaghi, Dan Rogozea, Rachel Cadle, Joseph Bustamante, Leni Moldovan, Horia I. Petrache and Nicanor I. Moldovan
Appl. Sci. 2022, 12(21), 11015; https://doi.org/10.3390/app122111015 - 31 Oct 2022
Cited by 6 | Viewed by 2599
Abstract
The middle ear bones (‘ossicles’) may become severely damaged due to accidents or to diseases. In these situations, the most common current treatments include replacing them with cadaver-derived ossicles, using a metal (usually titanium) prosthesis, or introducing bridges made of biocompatible ceramics. Neither [...] Read more.
The middle ear bones (‘ossicles’) may become severely damaged due to accidents or to diseases. In these situations, the most common current treatments include replacing them with cadaver-derived ossicles, using a metal (usually titanium) prosthesis, or introducing bridges made of biocompatible ceramics. Neither of these solutions is ideal, due to the difficulty in finding or producing shape-matching replacements. However, the advent of additive manufacturing applications to biomedical problems has created the possibility of 3D-printing anatomically correct, shape- and size-personalized ossicle prostheses. To demonstrate this concept, we generated and printed several models of ossicles, as solid, porous, or soft material structures. These models were first printed with a plottable calcium phosphate/hydroxyapatite paste by extrusion on a solid support or embedded in a Carbopol hydrogel bath, followed by temperature-induced hardening. We then also printed an ossicle model with this ceramic in a porous format, followed by loading and crosslinking an alginate hydrogel within the pores, which was validated by microCT imaging. Finally, ossicle models were printed using alginate as well as a cell-containing nanocellulose-based bioink, within the supporting hydrogel bath. In selected cases, the devised workflow and the printouts were tested for repeatability. In conclusion, we demonstrate that moving beyond simplistic geometric bridges to anatomically realistic constructs is possible by 3D printing with various biocompatible materials and hydrogels, thus opening the way towards the in vitro generation of personalized middle ear prostheses for implantation. Full article
(This article belongs to the Special Issue 3D Bioprinted Tissues for Personalized Medicine Approaches)
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11 pages, 4072 KiB  
Article
Design and Implementation of Anatomically Inspired Mesenteric and Intestinal Vascular Patterns for Personalized 3D Bioprinting
by Rachel Cadle, Dan Rogozea, Leni Moldovan and Nicanor I. Moldovan
Appl. Sci. 2022, 12(9), 4430; https://doi.org/10.3390/app12094430 - 27 Apr 2022
Cited by 1 | Viewed by 1858
Abstract
Recent progress in bioprinting has made possible the creation of complex 3D intestinal constructs, including vascularized villi. However, for their integration into functional units useful for experimentation or implantation, the next challenge is to endow them with a larger-scale, anatomically realistic vasculature. In [...] Read more.
Recent progress in bioprinting has made possible the creation of complex 3D intestinal constructs, including vascularized villi. However, for their integration into functional units useful for experimentation or implantation, the next challenge is to endow them with a larger-scale, anatomically realistic vasculature. In general, the perfusion of bioprinted constructs has remained difficult, and the current solution is to provide them with mostly linear and simply branched channels. To address this limitation, here we demonstrated an image analysis-based workflow leading through computer-assisted design from anatomic images of rodent mesentery and colon to the actual printing of such patterns with paste and hydrogel bioinks. Moreover, we reverse-engineered the 2D intestinal image-derived designs into cylindrical objects, and 3D-printed them in a support hydrogel. These results open the path towards generation of more realistically vascularized tissue constructs for a variety of personalized medicine applications. Full article
(This article belongs to the Special Issue 3D Bioprinted Tissues for Personalized Medicine Approaches)
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