Innovative Technologies for Detecting Antioxidant Properties and Oxidative Stabilization of Agri-Food Products and Plant Extracts

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Methods for Antioxidants Evaluation/Measurement".

Deadline for manuscript submissions: 30 November 2025 | Viewed by 3195

Special Issue Editor

1. School of Regional Innovation and Social Design Engineering, Faculty of Engineering, Kitami Institute of Technology, 165 Koen-cho, Kitami 090-8507, Hokkaido, Japan
2. RIKEN Centre for Advanced Photonics, RIKEN, 519-1399 Aramaki-Aoba, Aoba-ku 980-0845, Sendai, Japan
Interests: meat science; non-invasive detection; hyperspectral imaging; terahertz spectroscopy; green extraction; food safety
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Special Issue Information

Dear Colleagues,

Food provides essential amino acids, carbohydrates, proteins, vitamins, minerals, and other vital nutrients that support daily human activities, energy, and nutritional needs. From an economic perspective, recycling waste agri-food by-products can address environmental issues and provide valuable resources for extraction in various industries. The development of green extraction and formulation methods tailored to each antioxidant is crucial for maintaining the stability of active ingredients and ensuring effective delivery to their targets. These extracts are rich in bioactive compounds such as polyphenols and flavonoids, which possess significant antioxidant activity, as well as nutraceutical and biomedical benefits.

This Special Issue aims to highlight the use of cutting-edge technologies to detect the antioxidant properties and oxidative stability of agri-food products and plant extracts. We welcome all types of articles, including original research, numerical studies, and comprehensive reviews related to (but not limited to) these topics.

Note: In studies of complex mixtures of natural products, the characterization of chemicals using analytical methodologies, such as HPLC, MS, LC–MS, HPLC–MS, and NMR, should be included.

Dr. Chao Hui Feng
Guest Editor

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Keywords

  • green extraction
  • plant extracts
  • bioactive compounds
  • flavonoids
  • polyphenols
  • detection technologies
  • antioxidant activity

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Published Papers (3 papers)

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Research

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31 pages, 5335 KiB  
Article
Monitoring of Antioxidant Efficacy of Mangrove-Derived Polyphenols in Linseed Oil by Physicochemical and Fluorescence Methods
by Manjeet Singh, Eliot Botosoa and Romdhane Karoui
Antioxidants 2025, 14(2), 192; https://doi.org/10.3390/antiox14020192 - 7 Feb 2025
Viewed by 672
Abstract
This study was conducted to assess the antioxidant potential of polyphenolic extracts from Rhizophora mucronata and Avicennia marina as natural preservatives in comparison with synthetic butylated hydroxytoluene (BHT) and rosemary extract. Antioxidant activities were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), [...] Read more.
This study was conducted to assess the antioxidant potential of polyphenolic extracts from Rhizophora mucronata and Avicennia marina as natural preservatives in comparison with synthetic butylated hydroxytoluene (BHT) and rosemary extract. Antioxidant activities were assessed by 2,2-diphenyl-1-picrylhydrazyl (DPPH), oxygen radical absorbance capacity (ORAC), and total phenolic content (TPC). Extracts were blended into linseed oil and evaluated for oxidative stability using a 15-day Schaal oven test. Physicochemical analyses, including peroxide value (PV), acid index (AI), p-anisidine value (p-AnV), and thiobarbituric acid reactive substances (TBARS), showed that mangrove-treated oils exhibited the highest stability against oxidation compared to the negative and positive controls. R. mucronata mature leaves presented the highest DPPH inhibition (93.40%) and the lowest TBARS value (0.33 ± 0.0 mg MDA/kg of oil) on day 11. Fluorescence spectroscopy provided complementary and valuable information. Statistical analysis using factorial discriminant analysis (FDA) achieved a classification accuracy of 91.43%, underlining the different oxidative profiles of the treated samples. These findings demonstrated the potential of extracts from mangrove plants as a sustainable alternative to synthetic antioxidants for food preservation. Future studies should explore broader food applications using advanced analytical techniques to optimize their efficiency and performing a series of toxicity evaluations. Full article
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18 pages, 7289 KiB  
Article
Formulation, Characterization, Antibacterial Activity, Antioxidant Activity, and Safety Evaluation of Camphora longepaniculata Essential Oil Nanoemulsions Through High-Pressure Homogenization
by Yue Yan, Changhe Wei, Xin Liu, Xin Zhao, Shanmei Zhao, Shuai Tong, Guoyou Ren and Qin Wei
Antioxidants 2025, 14(1), 33; https://doi.org/10.3390/antiox14010033 - 30 Dec 2024
Viewed by 887
Abstract
The volatility, instability, and water insolubility of Camphora longepaniculata essential oil (CLEO) limit its practical applications in the food, pharmaceutical, and cosmetics industries. CLEO nanoemulsions (CLNEs) were formulated and characterized to overcome the aforementioned issues. The volatile compounds of CLEO were identified by [...] Read more.
The volatility, instability, and water insolubility of Camphora longepaniculata essential oil (CLEO) limit its practical applications in the food, pharmaceutical, and cosmetics industries. CLEO nanoemulsions (CLNEs) were formulated and characterized to overcome the aforementioned issues. The volatile compounds of CLEO were identified by gas chromatography–mass spectrometry. CLNEs were prepared using EL-40 (5%, w/w) as the surfactant via the high-pressure homogenization method. The formation of nanoemulsions was verified by Fourier transform infrared spectroscopy and transmission electron microscopy. Homogenized nanoemulsions had smaller particle sizes of 39.99 ± 0.47 nm and exhibited enhanced stability. The nanostructured CLEO showed an antibacterial effect against Escherichia coli and Staphylococcus aureus. The antibacterial mechanism was explored through bacterial morphology and intracellular lysate leakage. CLNEs disrupted the structure of bacterial cells and impaired the permeability of cell membranes, resulting in the leakage of bacterial intracellular contents. The nanoemulsions exhibited superior radical scavenging ability compared to the pure oil. Furthermore, evaluations of the cellular safety of the CLNEs demonstrated that the survival rate of exposed HOECs was greater than 90%, with an apoptosis rate of less than 10% in a concentration range. The results demonstrated that nanoemulsification improved the stability, solubility in aqueous media, and bioavailability of CLEO, thereby broadening its potential industrial applications as a natural antibacterial and antioxidant agent. Full article
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Review

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20 pages, 7772 KiB  
Review
Recent Advances, Challenges, and Functional Applications of Natural Phenolic Compounds in the Meat Products Industry
by Ting Bai, Xiulian Wang, Wenqing Du, Jie Cheng, Jiamin Zhang, Yin Zhang, Roungdao Klinjapo, Suvaluk Asavasanti and Patchanee Yasurin
Antioxidants 2025, 14(2), 138; https://doi.org/10.3390/antiox14020138 - 24 Jan 2025
Viewed by 1056
Abstract
Natural phenolic compounds (NPCs) have been proven to effectively extend the storage time of meat products in recent years. To promote the discovery of more NPCs and their applications, this review examines recent progress in the classification, antioxidant, and antibacterial mechanisms of NPCs [...] Read more.
Natural phenolic compounds (NPCs) have been proven to effectively extend the storage time of meat products in recent years. To promote the discovery of more NPCs and their applications, this review examines recent progress in the classification, antioxidant, and antibacterial mechanisms of NPCs used in meat products. These compounds are found in both edible and inedible parts of plants, including fruits, vegetables, and trees. The recycling of agricultural by-products aligns with green agricultural trends and serves as a guideline for developing new sources of natural additives. Studies on the application of NPCs in various livestock and poultry products, either directly mixed into the matrix or indirectly contacted by preparation into bioactive films and packaging materials, has highlighted the great potential of NPCs. The pro-oxidative effects of NPCs on proteins and their interactions with biological macromolecules, such as proteins, provide new ideas for in-depth research on antioxidant and antibacterial mechanisms. Full article
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