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Antioxidants
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Modern Analytical Approaches in the Identification of Polyphenols in Food,...
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Modern Analytical Approaches in the Identification of Polyphenols in Food, Food Sub-Products and Plant Matrices

A special issue of Antioxidants (ISSN 2076-3921). This special issue belongs to the section "Natural and Synthetic Antioxidants".

Deadline for manuscript submissions: 30 September 2025 | Viewed by 5847

Special Issue Editor

Dr. Alina Plenis

E-Mail Website
Guest Editor
Department of Analytical Chemistry, Faculty of Pharmacy, Medical University of Gdansk, 80-416 Gdansk, Poland
Interests: bioactive phenolic compounds; extraction process; analytical techniques; plant and food analysis; antioxidant; biological activity

Special Issue Information

Dear Colleagues,

Phenolic compounds are a large group of phytochemicals which are widely distributed in commonly consumed foods such as fruits, vegetables, whole grains, nuts, seeds, legumes, spices, coffee, tea, red wine, cocoa, and virgin olive oil. To date, more than 8000 naturally occurring phenolic compounds have been reported in plants. Dietary polyphenols can potentially protect against various chronic diseases. The most important effects of phenolic compounds include antioxidant, antibacterial, anti-inflammatory, antiviral, cancer chemopreventive, cardiovasculo-protective, immunomodulatory, and hepatoprotective activities. Various literature reviews have demonstrated the potential protective effects of polyphenol-rich foods and their extracts toward cardiovascular diseases, certain cancers, and neurodegenerative diseases, mostly through antioxidant and chemo-preventive properties. Thus, current scientific research on polyphenols has aroused great interest and significantly attracted the attention of researchers.

This Special Issue of Antioxidants, titled “Modern Analytical Approaches in the Identification of Polyphenols in Food, Food Sub-Products and Plant Matrices”, aims to collect original current research on the health benefits of polyphenols and their identification in food, food sub-products, and plant matrices. Covered aspects can include, but are not limited to, different extraction procedures and advanced analytical techniques of the isolation and characterization of polyphenols.

We invite you to submit your latest research findings or review articles for this Special Issue. If you have any questions, please do not hesitate to contact us. We look forward to hearing from you.

Dr. Alina Plenis
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. Antioxidants is an international peer-reviewed open access monthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. The Article Processing Charge (APC) for publication in this open access journal is 2900 CHF (Swiss Francs). Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • phenolic compounds
  • food
  • plants
  • extraction process
  • biological activity
  • analytical techniques
  • human health

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Published Papers (3 papers)

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Research

16 pages, 2842 KiB  
Article
Polyphenolic Antioxidants in Bilberry Stems and Leaves: A Non-Targeted Analysis by Two-Dimensional NMR Spectroscopy and Liquid Chromatography–High-Resolution Mass Spectrometry
by Anna V. Faleva, Nikolay V. Ulyanovskii, Alexandra A. Onuchina and Dmitry S. Kosyakov
Antioxidants 2024, 13(11), 1409; https://doi.org/10.3390/antiox13111409 - 17 Nov 2024
Viewed by 1274
Abstract
Compared with those of berries, the stems and leaves of the genus Vaccinium are important and underestimated sources of polyphenols with high antioxidant activity. In the course of this work, aqueous methanol extracts of the aerial parts of common bilberry (Vaccinium myrtillus [...] Read more.
Compared with those of berries, the stems and leaves of the genus Vaccinium are important and underestimated sources of polyphenols with high antioxidant activity. In the course of this work, aqueous methanol extracts of the aerial parts of common bilberry (Vaccinium myrtillus L.) and bog bilberry (Vaccinium uliginosum L.) were studied to analyze the component compositions of their biologically active polyphenolic compounds. The aqueous methanol fractions of the stems and leaves of the studied samples contained 8.7 and 4.6% extractives, respectively, and were comparable in total polyphenol content, but presented significant differences in antioxidant activity. The identification of polyphenolic compounds was carried out via the following two-stage analytical procedure: (1) non-targeted screening of dominant structures via the 2D NMR method and (2) analysis of HPLC-HRMS data via the scanning of precursor ions for a specific ion. A total of 56 phenolic compounds were identified, including the glycosides quercetin, proanthocyanidins, and catechins, as well as various conjugates of caffeic and p-coumaric acids, including iridoids. Some of the latter, such as caffeoyl and p-coumaroyl hydroxydihydromonotropein, as well as a number of lignan glycosides, were described for the first time in V. uliginósum and V. myrtillus. Full article
(This article belongs to the Special Issue Modern Analytical Approaches in the Identification of Polyphenols in Food, Food Sub-Products and Plant Matrices)
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16 pages, 436 KiB  
Article
Characterization of Phenolic Profile and Biological Properties of Astragalus membranaceus Fisch. ex Bunge Commercial Samples
by Saba Shahrivari-Baviloliaei, Ilkay Erdogan Orhan, Nurten Abaci Kaplan, Agnieszka Konopacka, Krzysztof Waleron, Alina Plenis and Agnieszka Viapiana
Antioxidants 2024, 13(8), 993; https://doi.org/10.3390/antiox13080993 - 16 Aug 2024
Cited by 3 | Viewed by 1593
Abstract
Astragalus membranaceus Fisch. ex Bunge (syn. Astragalus mongholicus Bunge) is one of the notable medicinal and food plants. Therefore, the aim of this study was to calculate the phenolic composition and antioxidant, antimicrobial, as well as enzyme inhibitory [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and [...] Read more.
Astragalus membranaceus Fisch. ex Bunge (syn. Astragalus mongholicus Bunge) is one of the notable medicinal and food plants. Therefore, the aim of this study was to calculate the phenolic composition and antioxidant, antimicrobial, as well as enzyme inhibitory [acetylcholinesterase (AChE), butyrylcholinesterase (BChE), and tyrosinase (TYR)] activities with chemometric approaches of the hydromethanolic and water extracts of commercial A. membranaceus samples. Ten individual phenolic compounds were determined using high-performance liquid chromatography (HPLC), and only quercetin was found at a level of above 80 µg/g DW in both extracts. Moreover, the highest antioxidant activity in DPPH, FRAP, ABTS, and CUPRAC assays was found in the sample containing the roots in loose form from USA. A. membranaceus extracts displayed the inhibition zone diameters within the range from 10 to 22 mm antimicrobial activity against S. aureus, while there were no inhibition zones in any extracts in case of E. coli. The extracts of A. membranaceous showed an inhibition rate below 40% against TYR, and among tested extracts, only two samples were able to inhibit BChE with IC50 values of above 30 µg/mL. Correlation analysis showed a highly positive relationship between their phenolic composition and antioxidant activity. Concluding, the obtained results confirmed that A. membranaceus commercial samples could be an important dietary source of natural antioxidants. Full article
(This article belongs to the Special Issue Modern Analytical Approaches in the Identification of Polyphenols in Food, Food Sub-Products and Plant Matrices)
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23 pages, 1291 KiB  
Article
Characterization of the Metabolic Profile of Olive Tissues (Roots, Stems and Leaves): Relationship with Cultivars’ Resistance/Susceptibility to the Soil Fungus Verticillium dahliae
by Irene Serrano-García, Lucía Olmo-García, Olga Monago-Maraña, Iván Muñoz Cabello de Alba, Lorenzo León, Raúl de la Rosa, Alicia Serrano, Ana María Gómez-Caravaca and Alegría Carrasco-Pancorbo
Antioxidants 2023, 12(12), 2120; https://doi.org/10.3390/antiox12122120 - 15 Dec 2023
Cited by 2 | Viewed by 2233
Abstract
Verticillium wilt of olive (VWO) is one of the most widespread and devastating olive diseases in the world. Harnessing host resistance to the causative agent is considered one of the most important measures within an integrated control strategy of the disease. Aiming to [...] Read more.
Verticillium wilt of olive (VWO) is one of the most widespread and devastating olive diseases in the world. Harnessing host resistance to the causative agent is considered one of the most important measures within an integrated control strategy of the disease. Aiming to understand the mechanisms underlying olive resistance to VWO, the metabolic profiles of olive leaves, stems and roots from 10 different cultivars with varying levels of susceptibility to this disease were investigated by liquid chromatography coupled to mass spectrometry (LC-MS). The distribution of 56 metabolites among the three olive tissues was quantitatively assessed and the possible relationship between the tissues’ metabolic profiles and resistance to VWO was evaluated by applying unsupervised and supervised multivariate analysis. Principal component analysis (PCA) was used to explore the data, and separate clustering of highly resistant and extremely susceptible cultivars was observed. Moreover, partial least squares discriminant analysis (PLS-DA) models were built to differentiate samples of highly resistant, intermediate susceptible/resistant, and extremely susceptible cultivars. Root models showed the lowest classification capability, but metabolites from leaf and stem were able to satisfactorily discriminate samples according to the level of susceptibility. Some typical compositional patterns of highly resistant and extremely susceptible cultivars were described, and some potential resistance/susceptibility metabolic markers were pointed out. Full article
(This article belongs to the Special Issue Modern Analytical Approaches in the Identification of Polyphenols in Food, Food Sub-Products and Plant Matrices)
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