Spread of Multidrug-Resistant Microorganisms, 2nd Edition

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Guest Editor
Department of Pharmacy, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, Italy
Interests: antimicrobial, anti-biofilm and anti-virulence activities of bioactive substances of natural origin; semi-synthesis and innovative biomaterials; the research activity has concerned oral cavity microorganisms
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E-Mail Website
Guest Editor
Department of Pharmacy, University “G. d’Annunzio” Chieti-Pescara, 66100 Chieti, Italy
Interests: antimicrobial; anti-biofilm and anti-virulence activities of natural compounds alone and combined with antibiotics against multi drug-resistant strains (Helicobacter pylori, Staphylococcus spp., Pseudomonas aeruginosa, Candida albicans, Mycobacterium abscessus and other fast-growing mycobacteria)
Special Issues, Collections and Topics in MDPI journals

Special Issue Information

Dear Colleagues,

The first edition of the Special Issue “Spread of Multidrug-Resistant Microorganisms” was published in 2021. It is a successful collection comprising 11 excellent papers. This encouraged us to open a second edition on the same topic.

As a continuation of the first Special Issue, this second edition focuses on the epidemiology of MDROs, the activity of new antimicrobials, implication of various determinants of resistance and strategies to control the diffusions of MDROs. Researchers working in this field are encouraged to submit their best manuscript(s) to this Special Issue.

Dr. Mara Di Giulio
Dr. Silvia Di Lodovico
Guest Editors

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Keywords

  • multidrug-resistant organisms
  • MDR
  • antibiotic resistance gene
  • nosocomial infections
  • new marker of resistance

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Published Papers (1 paper)

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Research

19 pages, 1580 KiB  
Article
Fitness Burden for the Stepwise Acquisition of First- and Second-Line Antimicrobial Reduced-Susceptibility in High-Risk ESKAPE MRSA Superbugs
by Eleonora Chines, Gaia Vertillo Aluisio, Maria Santagati, Maria Lina Mezzatesta and Viviana Cafiso
Antibiotics 2025, 14(3), 244; https://doi.org/10.3390/antibiotics14030244 - 28 Feb 2025
Cited by 1 | Viewed by 2306
Abstract
Background: The fitness costs (FCs) of antimicrobial resistance (AMR) are crucial issues in antimicrobial resistance (AMR) onset, spread, and, consequently, public health. In Staphylococcus aureus, AMR can induce significant FCs due to slow growth, low competitiveness, and virulence. Here, we investigated the [...] Read more.
Background: The fitness costs (FCs) of antimicrobial resistance (AMR) are crucial issues in antimicrobial resistance (AMR) onset, spread, and, consequently, public health. In Staphylococcus aureus, AMR can induce significant FCs due to slow growth, low competitiveness, and virulence. Here, we investigated the genomics and FCs emerging for progressively acquiring daptomycin (DAP) and glycopeptide (GLY) reduced susceptibility in MRSA. Methods: Genomics was carried out using Illumina-MiSeq Whole-genome sequencing and bioinformatics. The biological FCs of isogenic MRSA strain pairs progressively acquiring DAP and GLY-reduced susceptibility, under DAP/GLY mono or combined therapy, were performed by in-vitro independent and competitive mixed growth, phenotypic in-vitro virulence analysis, and in-vivo G. mellonella larvae killing. Results: Genomics evidenced four different extremely resistant high-risk clones, i.e., ST-5 N315 HA-MRSA, ST-398 LA-MRSA, ST-22 USA-100 HA-EMRSA-15, and ST-1 MW2 CA-MRSA. In-vitro fitness assays revealed slow growth, lower competitiveness, and reduced virulence, predominantly in Galleria mellonella killing ability, in DAP-S hGISA, DAP-R GSSA, DAP-R hGISA, and DAP-R GISA strains. Conclusions: The occurrence of glycopeptide and daptomycin reduced susceptibility conferred increasing FCs, paid as a gradual reduction in virulence, competitiveness, and slow growth performance. Full article
(This article belongs to the Special Issue Spread of Multidrug-Resistant Microorganisms, 2nd Edition)
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