Advances in Reproductive Biotechnologies in Swine

A special issue of Animals (ISSN 2076-2615). This special issue belongs to the section "Pigs".

Deadline for manuscript submissions: 20 October 2026 | Viewed by 898

Editor


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Guest Editor
Professor Emeritus, Laboratory of Animal Reproduction, School of Veterinary Medicine, Azabu University, Kanagawa 252-5201, Japan
Interests: assisted reproduction technology; oocyte/embryo cryopreservation; reproduction in livestock

Special Issue Information

Dear Colleagues,

The primary purpose of raising pigs is pork production, with pig farming representing not only the most efficient protein supply system for humans but also a crucial means of converting byproducts generated in various food manufacturing processes into pork.

The factor that most significantly impacts pig farming productivity is reproductive efficiency, and reproductive technology is widely applied to enhance this. Reproductive technology can be broadly categorized into artificial insemination and embryo transfer technology, with various related techniques, such as semen and embryo preservation, having been developed.

Furthermore, in recent years, the value of pigs as experimental and medical animals has increased significantly. This is due to our ability to manipulate the genome of cells using CRISPR/Cas9 genome editing technology  and then combine reproductive technologies to achieve artificial modification of the pig genome at the individual level.

For this Special Issue, we welcome the submission of original research papers and reviews concerning all aspects of reproductive biotechnology in swine, with a particular focus on technologies that enhance reproductive efficiency and genetic improvement. 

Prof. Dr. Naomi Kashiwazaki
Guest Editor

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Keywords

  • pig
  • reproductive efficiency
  • reproductive technology
  • artificial insemination
  • embryo transfer
  • genome editing

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Published Papers (1 paper)

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Research

12 pages, 465 KB  
Article
Double Electroporation Combined with Zona Pellucida Removal Improves Biallelic Genome Editing Efficiency in Porcine Embryos
by Nanaka Torigoe, Takeshige Otoi, Manita Wittayarat, Oky Setyo Widodo, Theerawat Tharasanit, Kaywalee Chatdarong, Megumi Nagahara, Maki Hirata, Fuminori Tanihara and Zhao Namula
Animals 2026, 16(12), 1919; https://doi.org/10.3390/ani16121919 - 20 Jun 2026
Viewed by 485
Abstract
The CRISPR/Cas9 system has been widely used for gene editing in various species; however, mosaicism remains a significant challenge. This study aimed to improve gene editing efficiency and reduce mosaicism in porcine embryos by exploring double electroporation pre- and post-in vitro fertilization combined [...] Read more.
The CRISPR/Cas9 system has been widely used for gene editing in various species; however, mosaicism remains a significant challenge. This study aimed to improve gene editing efficiency and reduce mosaicism in porcine embryos by exploring double electroporation pre- and post-in vitro fertilization combined with zona pellucida (ZP) removal. We evaluated the effects of these treatments on the development and mutation rates of oocytes/zygotes edited with guide RNAs (gRNAs) targeting GGTA1, CMAH, or B4GALNT2 genes. Double electroporation significantly increased the total and biallelic mutation rates in ZP-intact zygotes but not in ZP-free zygotes edited using GGTA1-targeted gRNAs. All blastocysts from ZP-free zygotes exhibited biallelic mutations following double electroporation. For the CMAH gene, all blastocysts exhibited mutations (biallelic mutations ≥ 80%); however, double electroporation and ZP removal did not affect their mutation rates or efficiency. For the B4GALNT2 gene, double electroporation significantly increased total mutation rates in ZP-intact zygotes, whereas all blastocysts from ZP-free zygotes showed biallelic mutation. These findings suggest that double electroporation, particularly with ZP removal, may enhance gene-editing efficiency, reduce mosaicism and improve the success of genetic modifications. Full article
(This article belongs to the Special Issue Advances in Reproductive Biotechnologies in Swine)
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