Natural Antimicrobial Agents for Crop Protection and Enhancement Applications

A special issue of Agriculture (ISSN 2077-0472). This special issue belongs to the section "Crop Protection, Diseases, Pests and Weeds".

Deadline for manuscript submissions: 10 October 2026 | Viewed by 2398

Editors


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Guest Editor
State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, Lithuania
Interests: antimicrobial activity; biocontrol; metagenomic; killer systems; yeast; bacteria

E-Mail Website
Guest Editor
State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, Lithuania
Interests: killer yeasts; antibacterial; microbiota; antibiotic resistance; biocontrol

E-Mail Website
Guest Editor
State Scientific Research Institute Nature Research Centre, Akademijos St. 2, LT-08412 Vilnius, Lithuania
Interests: yeast; bacteria; killer toxin; biocidal systems; microbiota; bioactive nanoparticles

Special Issue Information

Dear Colleagues,

The use of agrochemicals has enabled higher yield and quality of the produce by supplying nutrients and protecting crops from pests, weeds, and diseases. However, the overuse of those chemicals poses hazards to human health and the environment; thus, the search and development of natural and non-hazardous antimicrobial agents is the leading task.

This Special Issue focuses on the employment and development of natural antimicrobial agents, including those produced by bacteria, yeasts, and fungi and derived from plants. Original research and innovative methods and technologies for the screening, development, and employment of natural compounds and/or isolated microorganisms to help prevent microbial hazards are welcome. Investigation of the broader applications of antimicrobial agents for crop enhancement, such as increasing soil fertility and improving microbiota biodiversity, crop yield, post-harvest stability, etc., is encouraged. Cutting-edge research on agriculturally relevant prebiotics, probiotics, synbiotics, and postbiotics is appreciated. All types of articles, such as original research, opinions, and reviews, are welcome.

Dr. Iglė Vepštaitė-Monstavičė
Dr. Bazilė Ravoitytė
Dr. Ramunė Stanevičienė
Guest Editors

Manuscript Submission Information

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Keywords

  • probiotics
  • epiphytes
  • endophytes
  • bacteria
  • yeasts
  • antimicrobial
  • antibacterial
  • essential oils
  • fermentation
  • metabolites

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Published Papers (1 paper)

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Review

30 pages, 1318 KB  
Review
Bacillus Species in Agriculture: Functional Traits, Biocontrol Performance, and Regulatory Safety Assessment
by Martynas Dėlkus, Algirdas Ivanauskas, Marija Žižytė-Eidetienė, Juliana Lukša-Žebelovič, Iglė Vepštaitė-Monstavičė, Sonata Brokevičiūtė and Neringa Šimkutė
Agriculture 2026, 16(4), 413; https://doi.org/10.3390/agriculture16040413 - 11 Feb 2026
Cited by 4 | Viewed by 1886
Abstract
Bacillus species are among the most widely used microbial agents in agricultural biocontrol, reflecting their ecological resilience, functional diversity, and long history of practical application. The antagonistic activity of Bacillus spp. against plant pathogens and their plant growth–promoting effects are well established. However, [...] Read more.
Bacillus species are among the most widely used microbial agents in agricultural biocontrol, reflecting their ecological resilience, functional diversity, and long history of practical application. The antagonistic activity of Bacillus spp. against plant pathogens and their plant growth–promoting effects are well established. However, these biological functions are frequently considered in isolation from safety evaluations and regulatory decision-making, resulting in a fragmented evidence base. This review addresses this gap by providing an integrated synthesis of agriculturally relevant Bacillus taxa, explicitly linking biocontrol performance with strain-level safety considerations and regulatory assessment. This review focuses on the principal groups currently applied in agriculture, including the Bacillus subtilis lineage, notably B. amyloliquefaciens, B. velezensis, B. pumilus, and B. licheniformis, as well as B. thuringiensis and Cytobacillus firmus. Key mechanisms underlying biocontrol efficacy are examined alongside evidence from greenhouse and field applications. These mechanisms include the production of secondary metabolites and volatile compounds, biofilm formation, rhizosphere colonisation, and the induction of plant defence responses. Attention is given to environmental and operational factors that influence the consistency of performance. A central contribution of this review is the integration of functional evidence with safety-relevant considerations, such as realistic metabolite exposure, antimicrobial resistance potential, and ecological effects. Regulatory approaches in the European Union, the United States, and selected Organisation for Economic Co-operation and Development countries are compared to illustrate how such evidence informs risk assessment and supports the sustainable use of Bacillus-based biocontrol agents in modern agriculture. Full article
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