Seeds, Volume 4, Issue 1 (March 2025) – 16 articles

Kentucky bluegrass (KBG) has poor seed establishment in the fall when used as a perennial groundcover in corn production. This study was conducted to investigate the effect of various seed treatments and soil amendments on the establishment of KBG under drought and non-drought conditions, simulated in a growth chamber. The effect of seed treatments, soil amendments, and irrigation frequency on KBG germination and shoot dry weight were measured over 21 days in a controlled environment at 21 °C, 50% RH, and exposure to a constant red light. The treatments were the Hydroloc seed treatment, a lime soil amendment, the Pivot Bio seed treatment, an ammonium nitrate soil amendment, a gibberellic acid seed treatment, osmotic seed priming, and an untreated control. The layout was a randomized complete block design, with two irrigation frequencies (restricted and full irrigation) and four replications (blocks). The irrigation treatments were applied to whole plots and the seed treatments were applied to subplots. The entire experiment was repeated four times. Irrigation affected the germination of all the seed treatments, but the size of the effect depended on the seed treatment applied. The control and Hydroloc treatments did not have significantly different dry shoot weights, while all the other treatments had significantly different dry shoot weights when comparing the irrigation regimes. The Hydroloc treatment significantly outperformed all the other treatments in regard to the restricted and full irrigation regime. These results indicate that the Hydroloc seed treatment improves KBG germination and shoot dry weight in drought and non-drought conditions, promoting KBG establishment in a wide range of soil moisture conditions. Full article

Mineral malnutrition negatively impacts almost 30% of the world population and could result in anemia, blindness, and stunted growth. Seed traits involve nutritional, physical, and physiological characteristics that play a key role in achieving seed quality. Chickpea (Cicer arietinum), the second most important food legume crop worldwide, is characterized by its rich nutrient profile, fiber, and antioxidant content. However, seed quality traits composition and daily nutritional value in chickpea seeds remain largely unknown. The objectives of the current study were to determine the variability in concentrations of macro and micronutrients, oil, protein, their correlations, and daily nutritional values (% DV) in the seeds of 61 chickpea genotypes. The results demonstrate substantial variation among chickpea genotypes in most nutrients, protein, and oil content. Moreover, this study identifies 12 promising chickpea genotypes that indicate large nutrient content and potential usage in chickpea biofortification and breeding programs. Our results further show that consumption of 100 g chickpea seeds could provide 122% DV of manganese (Mn), 77% DV of copper (Cu), 43% DV of magnesium (Mg), 32% DV of phosphorus (P), 27% DV of zinc (Zn), 26% DV of iron (Fe), and 5% DV of calcium (Ca). Overall, these findings improve our understanding of chickpea seed nutrient content as well as global food security by combatting mineral malnutrition. Full article

Seed priming is a simple, inexpensive, and effective pre-sowing technique that enables plants to better tolerate abiotic stresses, including high soil salinity, which is a major limiting factor in the establishment of halophytes for saline agriculture, as germinating seeds and early seedlings of many halophytes are sensitive compared to the mature vegetative stage. This article attempts to provide an overview of the research on the seed priming effects on halophyte seeds and subsequent seedlings/plants. Different physio-chemical and molecular processes, including the induction of priming/stress memory, which enhance salinity tolerance following seed priming, have also been discussed. This review also covers the aspects of reactive oxygen species (ROS), and nitric oxide (NO) signaling(s) that are activated as a result of seed priming. Finally, the limitations and prospects of seed priming to enhance the agronomic potential of halophytes for saline agriculture have been discussed. Full article

The cost of producing organic crops is increasing. Agricultural wastes can be used as biostimulants to increase plant growth and productivity and reduce the dependence on chemical fertilizers. A pouch assay and a potted greenhouse experiment were conducted to investigate the effect of pyroligneous acid (PA) and sea lettuce (SL) on kale (Brassica oleracea subsp. acephala (DC.) Metzg) seed germination and growth. Although previous studies have demonstrated that these two biostimulants could promote plant germination and growth, there is little research to compare their effects on seed germination and plant growth. The pouch assay showed that PA liquid affected the seed germination rate under different concentrations; the seed germination rate decreased as the concentration of PA liquid increased. However, the effect of seed germination was less pronounced in SL liquids. Kale seeds treated with 0.01% PA showed the best elongation and seedling growth performance. Moreover, the greenhouse experiment indicates that SL liquids significantly (p < 0.05) affected kale growth production, while PA liquid had less difference on kale growth under various concentrations. The 0.25% PA and 1% SL increased the aboveground fresh weight by ca. 26% and 29%, respectively. Also, the phytochemical contents of kale leaves, including phenolics, flavonoids, ascorbate, and protein, were significantly increased with 0.25% PA and 1% SL application. These results suggest that low concentrations of PA are more suitable for seedling root growth in kale and 1% SL had the most significant growth-promoting effect on kale. Hydrothermal carbonization sea lettuce liquid can be used as a good biostimulant for agricultural production to improve kale germination and growth. Full article

Insect-based (silkworm cocoons) and plant-based (cotton wool pads and gauzes) fiber substrates were used to support and ameliorate seed germination originating from trifoliate orange (Poncirus trifoliata) and pomegranate (Punica granatum) trees. Three different commercial formulations of beneficial microorganisms (Bacillus spp.-Azotobacter spp., Saccharomyces boulardii, and Saccharomyces cerevisiae) were administered to seeds in order to evaluate their contribution to germination and growth. The silkworm cocoons provided better germination rates for P. trifoliata seeds (83.33%) among the tested media without any microbial supplementation. These rates increased towards the absolute maximum (100%) when Bacillus spp.-Azotobacter spp., S. boulardii and S. cerevisiae were applied. Furthermore, inoculums of Bacillus spp.-Azotobacter spp. 2 mL and S. cerevisiae 3 g raised the pomegranate seed germination ability by 30–33.33% and 50–67.7%, respectively, on silkworm cocoon substrates when compared to plant-derived, cellulosic fiber substrates under the same biotic exposure. On increasing the size of applied microbial inoculums, seed germination moved from optimum to suboptimum for all germination media. Examination of multipartite pH compatibility (between seeds, microorganisms, and germination media) was beneficial and of functional value. In conclusion, the germination rates of both tree species can be raised using bacterial and yeast supplementation, including medical-grade S. boulardii, on environmentally friendly materials such as insect- and plant-based fiber substrates. Full article

Azadirachta indica (Neem tree) has become widely naturalised and invasive across many countries and regions including northern Australia. To aid management of A. indica where it has become a weed, a series of studies were undertaken to determine its potential soil seed bank persistence. In a field trial, packets of seeds were buried, retrieved periodically over two years and the seed viability assessed. Viability declined rapidly, with a single viable seed retrieved after 12 months burial and none thereafter. Burial depth, soil type, and pasture cover (present and excluded) significantly influenced viability (%) at 3- and 6-month retrievals. Similar data were obtained from repeated runs of a controlled ageing laboratory experiment, which categorized seeds as forming a ‘transient’ seed bank. In a third trial, fresh fruits were placed on the soil surface in replicated field enclosures over two consecutive years and seedling emergence monitored fortnightly. In both years there was no emergence from pasture excluded soil plots and emergence ceased after 2.3 and 8.4 months in plots with pasture present. A fourth (glasshouse) trial found most seeds will emerge from the soil when buried from 1 to 4 cm. However, more fatal germination than successful emergence was recorded for seeds buried at 8 cm. Seed desiccation and fatal germination are factors in A. indica developing a transient soil seed bank, and infestations require shorter-term control programs where seed input is prevented. Full article

The foxtail millet exhibits a diverse range of hull colors, which are crucial indicators for assessing its nutritional and economic value. However, the molecular regulatory mechanisms that govern the hull color of foxtail millet are largely unknown at present. This gap in knowledge significantly impedes efforts to enhance the quality traits of foxtail millet. This study utilized a population of 250 F₆ recombinant inbred lines (RILs) generated from a cross between two foxtail millet varieties: Yugu18 (with light yellow seeds) and Hongjiugu19 (with red seeds). Four methods, the visual grouping method (I), the visual colorimetric method (II), the Lab determination method (III), and the RGB determination method (IV), were employed to determine the hull color of each line across four environments and QTL identification were conducted subsequently. It showed that there were 10, 12, 69 and 56 QTLs were detected for hull color through four methods, and these QTLs were integrated into 4, 6, 27 and 25 unique QTLs, respectively. There were three, four, four and four major QTLs. Of which, three major QTLs (qHC1.1, qHC1.2 and qHC9.3) on chromosomes 1 and 9 could be detected by all 4 methods. qHC9.1 was detected by all four methods except for method I. There were also one, one, seven and four minor identity QTLs identified across the 4 methods. Four minor QTLs (qHC3.1, qHC3.3, qHC4.1 and qHC5.1) can be stably detected only in method III, and two minor QTLs (qHC8.2 and qHC9.2) can be stably detected only in method IV. Generally, method I is fast, efficient and cost-effective, which is suitable for the rapid detection of hull color. Method II is also low-cost; however, it can detect more QTL for hull color, making it suitable for identifying major QTL loci in large populations. Methods III and IV can map more minor QTL and are more accurate in hull color characterization. This study identified four important hull color QTL for foxtail millet, which largely align with those reported in previous research. These findings establish a foundation for characterizing hull color indices and further advancing QTL mapping for grain color. Full article

The chemical quality of fenugreek seeds is a critical factor influencing their nutritional value, medicinal properties, and suitability for agricultural and industrial applications, making it essential to evaluate their biochemical composition and functional potential. This study evaluated the effects of salinity and different fertilization types on seed quality, early growth parameters, and key biochemical characteristics during fenugreek (Trigonella foenum-graecum L.) cultivation. A field experiment was established at the Agricultural University of Athens during the cropping period 2019–2020 (Year A) and 2020–2021 (Year B) in a split-plot design with the five main fertilization treatments Biocyclic-Vegan Humus Soil (BVH), Farmyard Manure (FM), Organic Compost (OC), Inorganic Fertilizer (IF; 11-14-14), and No Treatment Control (NTC) and two treatments: Elevated Salinity (ES) and Normal Salinity (NS). Fertilization significantly influenced various agronomic traits and seed compositions across both experimental years. The evaluation showed that organic fertilization with BVH yielded the best results among the treatments studied. The BVH × NS treatment consistently produced the highest plant height, seed protein content, seed yield, and mineral content, including nitrogen, calcium, and potassium, compared to the other treatments. ES impacted the concentrations of several elements, namely N (%), Ca (g/100 g), K (g/100 g), Fe (ppm), P (%), and Mg (g/100 g). Salinity also affected certain traits, such as Seed Total Ash and Dry Matter content, with significant interactions observed. These findings highlight the critical role of fertilization, especially organic fertilization, in improving both agronomic performance and seed nutritional quality in the studied crops, as well as in mitigating the adverse effects of salinity. Full article

Downy mildew caused by the oomycete Pseudoperonospora cubensis is a devastating disease of cucurbits. Cucumis species are attacked by pathotype 3 (clade 2) of the pathogen, while Cucurbita species are attacked by pathotype 6 (clade 1). The Sikkim-type cucumbers PI 197088 and PI 330628 express high levels of resistance against both pathotypes (clades) of the pathogen but no green-fruit cucumber cultivars resistant to the disease are available on the market. Here we report on several non-Sikkim accessions of cucumber that show resistance against downy mildew in four consecutive seasons. Mean % foliage attacked with downy mildew in the susceptible controls Ilan and SMR-18 was 93% and 71%, respectively, as against 0.2% and 1.8% in the Sikkim-type resistant controls PI 197088 and PI 330628, respectively. Twenty-four green fruit accessions were significantly more resistant than the susceptible cucumber controls. Five accessions showed less than 10% infected leaf area with downy mildew as follows: PI 432870—5%, PI 390266—7.5%, PI 418964—8.5%, PI 390258—8.8%, and G12—10%. PI 390258 and PI 390266 were susceptible to race 1 of powdery mildew but resistant to race 2, whereas PI 418964 was resistant to both races. These accessions may be used in breeding programs to accelerate the production of green-fruit, disease-resistant cucumbers. Full article

Herbaceous peonies, specifically the Balkan–Anatolian Paeonia peregrina Miller, are species with various uses such as ornamental and garden purposes, or they can be cut as flowers or potted, or they can be eaten or used for medicinal purposes due to the rich nutritional content of their seeds. However, conventional propagation methods, including rhizome division, grafting, and layering, are slow, while seed propagation is challenging due to double morpho-physiological dormancy. This study therefore evaluated the in vitro germination potential of P. peregrina seeds in darkness under different culture conditions, including different temperature regimes (constant at 15 °C or alternating from 22 °C to 15 °C); incubation periods [120 days: 22 °C (14 d) to 15 °C (0–105 d); 120 days: 22 °C (33 d) to 15 °C (0–87 d); 90 or 140 days at 15 °C]; seed cold storage period (none; 30 days; 3 months; or 2, 5, and 8 years); and gibberellic acid (GA₃) concentrations (0, 250, 500, 750, and 1000 mg L⁻¹), as dormancy release methods. The results indicated that 60-day-stored seeds (30 d at 15 °C and 30 d cold-storaged at 4–5 °C) exhibited 100% germination within an 80-day culture under 250 mg L⁻¹ GA₃ at 15 °C. A lower and constant temperature of 15 °C, a shorter 30-day seed cold storage period, and the lowest GA₃ concentration of 250 mg L⁻¹ comprised the most effective combination treatment for dormancy release and germination acceleration. An understanding of the underlying mechanisms of seed dormancy removal is imperative for successful germination, growth rate and seedling establishment, shortened breeding cycles, and germplasm conservation, leading to the field cultivation and economic production of these peony plants. Full article

Corn residual herbicides offer a practical approach to comprehensive weed management throughout the growing season. However, the use of residual pre-emergence herbicides can have a negative impact on crops grown in succession or within a rotation. A study was carried out to determine the effect of the residual activity of selected corn herbicides on soybeans. The objective of the study was to evaluate the impact of these herbicides on the germination of inoculated soybean seeds. Experiments were conducted in greenhouse conditions to check the carryover effect on soybean germination. Treatment combinations of two pre-herbicides and two inoculants were applied: atrazine (2241 g ai ha⁻¹), mesotrione (105 g ai ha⁻¹), and Bradyrhizobium japonicum, Bradyrhizobium japonicum + Bacillus subtilis, respectively. A randomized complete block design evaluated six treatment combinations, including the control. All treatments, except uninoculated treatments, presented efficacy in reducing the carryover effects of corn residual herbicides on the germination of soybeans. An increase in final germination percentage was observed with Bradyrhizobium japonicum + Bacillus subtilis co-inoculation plus atrazine (24% increase) and Bradyrhizobium japonicum plus mesotrione treatment combinations (19% increase). Inoculating soybean seeds with rhizobium bacteria can reduce the carryover effects on the germination of soybean seeds grown in soil applied with atrazine and mesotrione. Full article

Industrial hemp is mainly cultivated for its fibers aimed at the production of textiles, paper, and cordage; the inflorescences for medicinal purposes; and the seeds are used by the food industry due to their high nutritional and functional matrix of protein, fiber, lipids, and microelements. Hemp seed oil (HsO) is a unique source of polyunsaturated fatty acids, with a phenomenal ω6:ω3 ratio of 2.5–3.0, significantly enhancing human health when consumed daily. HsO is mostly obtained through cold pressing due to minimal thermal treatment, and although of lower yield compared to solvent extraction, it presents higher quality lipid fractions and organoleptic characteristics such as color, taste, flavor, and density. Although HsO is a powerful source of polyunsaturated fatty acids, antioxidants, and phytosterols, its production lacks standardized quality control parameters, except for THC, which is subject to EU legislation. Therefore, it is essential to build up a quality protocol system for standardizing seed conservation, oil extraction methods, and quality parameters. This review aims to display an overall nutritional framework of the HsO and encourage further research into its use in the food value chain. Full article

Horticulture is mainly based on transplanting seedlings produced by specialized nurseries. The recent European authorization of frass in organic farming presents new opportunities for the development of organic seedling production. Frass, a by-product of insect farming, offers innovative solutions for this sector. It mainly consists of insect excrement, exuviae, and uningested feed. Their fertilizing and biostimulating effects have been demonstrated in various pot and field crops experiments. However, the current knowledge regarding the application of frass in seedling production remains insufficient. This study aims to assess the optimal dose of mealworm frass in germination substrates for Allium cepa L., Beta vulgaris L., and Brassica rapa L. Germination and phytotoxicity tests were carried out, with seedlings evaluated one month after sowing in substrates containing frass at concentrations of 0.5%, 1%, 2%, and 3% of frass. The germination test revealed that the dilution of the frass at 1:100 produced a phytostimulant effect on A. cepa and a moderate phytotoxic effect on B. vulgaris and B. rapa. The application of mealworm frass at a concentration of 0.5–1% was generally the most effective dose, although all doses of frass in the substrate resulted in seedlings whose root length, leaf length, number of leaves, and biomass were significantly higher than the control. In conclusion, the application of low doses of mealworm frass in organic seedling production is promising and allows the management of potential phytotoxicity. Full article

The germination speed and completeness of bitter gourd seeds were enhanced using two seed coat treatments: scarification and removal of the seed coat. In comparison to the scarified seed and control, removing the seed coat improved the germination speed and completeness, making it the most successful method. When there are not enough seeds or when a research project needs a full, homogenous group of seedlings, this method would be the most helpful. The seed image analyzer, which can capture the small variations in seed parameters between groups, showed a triphasic pattern of water uptake during the imbibition process of germination of bitter gourd seeds, which was in accordance with the conventional methods of analyzing it. This was shown as a correlation between the mean germination rate and the perimeter of seeds. Full article

Canadian buffaloberry (Shepherdia canadensis (L.) Nutt.) is a perennial shrub known for its drought tolerance, nitrogen-fixing ability, and suitability for land reclamation and vegetation, particularly on nutrient-poor industrially disturbed soils in Alberta, Canada. Despite its ecological importance, commercial nurseries and greenhouses face challenges due to limited knowledge of optimizing seed germination and maintaining genetic diversity in cultivated seedlings. In this study, we investigated the interactive effect of cold stratification duration (0, 2, 4, 8, 12, and 16 weeks) and scarification time (control (no scarification), 1, and 4 h) on buffaloberry seed germination. The seed germination rate was tested using a factorial experiment with two factors arranged in a completely randomized design with four replications. Our findings indicate that scarification with a low concentration of acid for 1 h significantly enhances germination outcomes and shortens the required stratification period from 16 weeks to 12 weeks, achieving an optimal germination rate of 82%. Our novel approach using low-concentration acid to scarify Canadian buffaloberry seeds will help commercial greenhouses and forest nurseries improve seed germination, which utilizes this species for land reclamation and reforestation. Furthermore, this method can be adapted to improve germination in other native species with similar dormancy challenges, broadening its applications in ecological restoration efforts. Full article

Rice (Oryza sativa L.) is one of the most crucial cereal crops worldwide, serving as a staple food for a significant portion of the global population. Rice is the second most important staple food crop in Pakistan after wheat, and it is also a major export commodity. Concerning this, the current study aimed to evaluate the effects of different seed rates on the yield and yield-contributing parameters of rice varieties. The experiment was conducted over two consecutive kharif summer seasons, from 2020–21 and 2021–22, at the Pakistan Agricultural Research Council (PARC) Rice Program experimental area in Kala Shah Kaku, Lahore, Pakistan, by following a factorial randomized complete block design with three replications using coarse rice (KSK-133) and fine rice (Super Basmati) varieties. Different seed rates, including 27 kg/ha, 22 kg/ha, 17 kg/ha, and 12 kg/ha, were tested during the experiment. Different growth and yield-related attributes, such as plant height (cm), the number of productive tillers per plant, panicle length (cm), the number of grains per panicle, and grain yield (m⁻²), were recorded. The results showed that for KSK-133 and Super Basmati, the maximum grain yield was achieved at a sowing rate of 27 kg/ha in direct seed rice (DSR). The lowest yield was observed at a seeding rate of 12 kg/ha for KSK-133 and Super Basmati in DSR. Both basmati (Super Basmati) and coarse-grain (KSK-133) varieties exhibited similar responses to seed rate treatments, with the optimal performance observed at the highest seed rate of 27 kg/ha for both seasons. Grains per panicle and thousand grain weight emerged as critical determinants of yield, highlighting the need to balance vegetative growth with reproductive development. Breeding programs should focus on developing varieties that balance vegetative traits like tiller production and panicle length with reproductive traits to enhance overall yield. Based on these findings, it is concluded that using an optimal seeding rate of 27 kg/ha for direct-seeded fine and coarse rice varieties is beneficial in terms of tillers and higher yield. Full article