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Chickpea seed quality is highly susceptible to mechanical damage during handling and to rapid deterioration under postharvest storage. Atmospheric pressure Non-Thermal Plasma (NTP) has shown positive effects on seed quality in several species, but its long-term impact on chickpea remains poorly understood. This study evaluated the effect of NTP on the physiological germination process and postharvest deterioration of Cicer arietinum L. (Fabaceae) ’Felipe UNC-INTA’ seeds. Seeds were treated for three minutes with dielectric barrier discharge using O2 and N2 as carrier gases. Results showed that NTP optimized the triphasic germination response in embryo, especially in phases II and III, where radicle protrusion occurred earlier in treated (27 and 30 h) than in control (33 h) seeds, accompanied with a partition ratio < 1, indicating the roots’ preferential assimilate allocation. Fungal incidence decreased notably, e.g., Aspergillus decreased from 31% (control) to 11% (N2) and 10% (O2). O2-treated seeds exhibited higher germination (94%) than the control (90%) and an 11% reduction in individual electrical conductivity, indicating enhanced membrane integrity. After six months of storage, both treatments delayed aging, maintaining higher vigor than untreated seeds. Overall, NTP emerges as a promising postharvest technology to enhance and preserve seed vigor and viability in C. arietinum.

17 December 2025

(a) Diagram of the Dielectric Barrier Discharge (DBD) plasma system and measurement circuit, and (b) image of the DBD plasma system during the treatment of chickpea (Cicer arietinum L., Fabaceae, ‘Felipe UNC-INTA’) seeds. 1: Needle array active electrode, 2: seeds, 3: dielectric barrier, 4: grounded electrode, 5: plasma active zone, 6: gas injection system, 7: connection to the high-voltage transformer.

Soil salinity poses a major threat to agriculture by severely limiting how well plants grow and produce crops. It strongly inhibits seed germination, a critical stage for plant life. Thus, it is critical to understand the complex ways salinity affects seed germination at the physiological, biochemical, and molecular levels to develop effective salt stress mitigation strategies. This review synthesizes the underlying mechanisms of how salinity inhibits seed germination, the observed impacts of this inhibition, and potential mitigation strategies. The review revealed that high salt concentrations reduce seed germination percentage and increase germination time through multiple mechanisms. They create osmotic stress that reduces water uptake, cause ion toxicity that disrupts critical metabolic activities, and induce oxidative stress. Furthermore, salinity can modify endogenous hormonal profiles, specifically by decreasing germination stimulants like gibberellic acids while increasing inhibitors like abscisic acid. The review finally explored the strategies to mitigate salinity’s adverse effects on seed germination. They include seed priming, a technique involving partial hydration of seeds in an eliciting solution, a promising biotechnological tool to overcome salinity problems during seed germination. Other approaches are the use of organic amendments and the breeding of salt-tolerant varieties. Future research should combine conventional and advanced molecular technologies to develop salt-tolerant cultivars to ensure food security in salt-affected agricultural lands.

22 December 2025

Hot-Air Drying Temperature Affects Physiological Performance and Cyto(geno)toxic Endpoints in Soybean Seeds

  • Daynara Martins da Silva,
  • Tathiana Elisa Masetto and
  • Leilaine Gomes da Rocha
  • + 3 authors

Soybeans are widely used in agro-industrial sectors, and global demand for this crop continues to rise. After harvest, however, soybean seeds often lack the appropriate moisture content for storage, making drying a common practice under changing climate conditions. Because temperature is a critical factor during drying, this study aimed to evaluate the effect of air-drying temperature on physiological responses and cytogenetic conformation of soybean seeds. The experiment was conducted under a completely randomized design with four replications for each temperature. Seeds with 23 percent moisture content were dried in a convective dryer equipped with airflow and temperature control at 40 °C, 50 °C, 60 °C, and 70 °C until reaching 13 percent. Samples for physiological and cytological analyses were collected before and after drying. The results indicated that drying temperature influenced seed performance and vigor. Moreover, nuclear alterations were identified as an important component of the genotoxicity caused by high drying temperatures. Overall, air temperatures above 50 °C induced physiological and cytogenotoxic effects, underscoring the need for careful monitoring during seed drying.

12 December 2025

Agricultural research has accelerated in recent years, but farmers often lack the time and resources to conduct on-farm experiments, as most of their efforts are devoted to crop production. Seed classification provides essential insights for seed quality control, impurity detection, and yield estimation. Early identification of seed types is critical to reduce costs, minimize risks of poor field emergence, and support efficient crop management. Traditional classification methods rely heavily on manual feature extraction and expert input, which limits scalability and accuracy when dealing with highly similar seed types. To address this challenge, we propose an automated end-to-end deep learning framework for complex multiclass Brassica seed classification. Our framework integrates preprocessing, feature learning, and classification into a unified pipeline, eliminating the need for handcrafted features. Using a newly collected dataset of ten Brassica seed classes characterized by high texture similarity, we develop and evaluate a convolutional neural network optimized through architectural design and hyperparameter tuning. Experimental results demonstrate that the proposed framework achieves a classification accuracy of 93%, outperforming several state-of-the-art pretrained models. These findings highlight the potential of automated end-to-end deep learning models to enhance precision agriculture, providing robust and scalable solutions for seed quality monitoring and agricultural productivity.

9 December 2025

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Seed Priming Approaches That Achieve Environmental Stress Tolerance
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Seed Priming Approaches That Achieve Environmental Stress Tolerance

Editors: Jose Antonio Hernández Cortés, Gregorio Barba-Espín, Pedro Diaz-Vivancos

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