Abstract
Postharvest operations are cost intensive in microalgae production, and when electrocoagulation–electroflotation (EC/EF) with aluminum anodes is used, aluminum can remain associated with biomass and wash streams; hence, a selective postwash process is needed. Accordingly, this study defined an operational window for aluminum desorption that preserves the energetic advantage of EC/EF. A response-surface design (I-optimal/CCD) was used to evaluate the effects of the EDTA concentration (1–100 mM), contact time (5–20 min), mixing speed (100–300 rpm), and pH (6–10) on EC/EF-harvested Chlorella sp. biomass, with ANOVA and model diagnostics supporting adequacy. EDTA concentration and mixing emerged as significant factors, whereas time and pH acted mainly through interactions; moreover, quadratic terms for EDTA and mixing indicated diminishing returns at high levels. Consequently, the surface predicted an optimum near EDTA ≈ 65 mM, time ≈ 20 min, pH 10, and 100 rpm, corresponding to ~97% aluminum removal. Importantly, a confirmation run under these conditions across eight chlorophyte strains consistently achieved >95% removal, revealing narrow dispersion yet statistically distinguishable means. Taken together, coupling EC/EF with an EDTA postwash operation in the identified window effectively limits aluminum carry-over in microalgal biomass and, therefore, provides a reproducible basis for downstream conditioning and potential recirculation within biorefinery schemes.