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Article

Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials

1
Centro NanoMat, Facultad de Química, Instituto Polo Tecnológico de Pando, DETEMA, Universidad de la República, Montevideo 11800, Uruguay
2
Área de Microbiología, Departamento de Biociencias, Facultad de Química, Universidad de la República, Montevideo 11800, Uruguay
3
Tecnológico Nacional de México en Celaya, Guanajuato 38010, Mexico
*
Author to whom correspondence should be addressed.
Academic Editor: Simona Concilio
Chemistry 2021, 3(4), 1271-1285; https://doi.org/10.3390/chemistry3040092
Received: 30 September 2021 / Revised: 29 October 2021 / Accepted: 2 November 2021 / Published: 4 November 2021
(This article belongs to the Special Issue Recent Advances in Antimicrobial Materials)
Microbial technology offers a green alternative for the synthesis of value-added nanomaterials. In particular, fungal compounds can improve silver nanoparticle production, stabilizing colloidal nanoparticles. Based on a previous study by our group, silver nanoparticles obtained using the extracellular cell-free extracts of Phanerochaete chrysosporium (PchNPs) have shown antimicrobial and antibiofilm activity against Gram-negative bacteria. Moreover, nisin—a bacteriocin widely used as a natural food preservative—has recently gained much attention due its antimicrobial action against Gram-positive bacteria in biomedical applications. Therefore, the aim of this work was to conjugate biogenic silver nanoparticles (PchNPs) with nisin to obtain nanoconjugates ([email protected]) with enhanced antimicrobial properties. Characterization assays were conducted to determine physicochemical properties of [email protected], and also their antibacterial and antibiofilm activities were studied. The formation of [email protected] was confirmed by UV-Vis, TEM, and Raman spectroscopy analysis. Different [email protected] nanobioconjugates showed diameter values in the range of 60–130 nm by DLS and surface charge values between −20 and −13 mV. Nisin showed an excellent affinity to PchNPs, with binding efficiencies higher than 75%. Stable synthesized [email protected] nanobioconjugates were not only able to inhibit biofilm formation by S. aureus, but also showed inhibition of the planktonic cell growth of Staphyloccocus aureus and Escherichia coli, broadening the spectrum of action of the unconjugated antimicrobials against Gram-positive and Gram-negative bacteria. In conclusion, these results show the promising application of [email protected], prepared via green technology, as potential antimicrobial nanomaterials. View Full-Text
Keywords: biogenic nanoparticles; nanobioconjugates; nisin; antimicrobial; antibiofilm biogenic nanoparticles; nanobioconjugates; nisin; antimicrobial; antibiofilm
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MDPI and ACS Style

Zimet, P.; Valadez, R.; Raffaelli, S.; Estevez, M.B.; Pardo, H.; Alborés, S. Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials. Chemistry 2021, 3, 1271-1285. https://doi.org/10.3390/chemistry3040092

AMA Style

Zimet P, Valadez R, Raffaelli S, Estevez MB, Pardo H, Alborés S. Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials. Chemistry. 2021; 3(4):1271-1285. https://doi.org/10.3390/chemistry3040092

Chicago/Turabian Style

Zimet, Patricia, Ruby Valadez, Sofía Raffaelli, María Belén Estevez, Helena Pardo, and Silvana Alborés. 2021. "Biogenic Silver Nanoparticles Conjugated with Nisin: Improving the Antimicrobial and Antibiofilm Properties of Nanomaterials" Chemistry 3, no. 4: 1271-1285. https://doi.org/10.3390/chemistry3040092

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