Abstract
Acquisition and development of the oral microbiome are dynamic processes that occur during early life. However, data regarding longitudinal assembly and determinants of the infant oral microbiome are sparse. This study aimed to characterise temporal development of the infant oral microbiome during the first two years of life. Infant oral samples (n = 667 samples, 84 infants) were collected at 2–7 days and 1, 2, 3, 4, 5, 6, 9, 12, and 24 months of age using COPAN E-swabs. Bacterial DNA profiles were analysed using full-length 16S rRNA gene sequencing. At 4 months of age, 76.2% of infants were exclusively breastfed, while breastfeeding rates were 83.3% at 6 months and 65.5% at 12 months. The median breastfeeding duration was 12 months (IQR: 3 months). In this cohort, the oral microbiome was dominated by Streptococcus mitis, Gemella haemolysans, and Rothia mucilaginosa. Bacterial richness decreased significantly from 1 to 2 months, then rose significantly from 12 to 24 months. Shannon diversity increased from 1 week to 1 month and again from 6 to 9 months and 9 to 12 months (all p ≤ 0.04). Microbiome composition was significantly associated with multiple factors, including pacifier use, intrapartum antibiotic prophylaxis, maternal allergy, pre-pregnancy BMI, siblings, delivery mode, maternal age, pets at home, and birth season (all p ≤ 0.03). Introduction of solid foods was a significant milestone in oral microbiome development, triggering an increase in bacterial diversity (richness p = 0.0004; Shannon diversity p = 0.0007), a shift in the abundance of seven species, and a change in beta diversity (p = 0.001). These findings underscore how the oral microbiome develops over the first two years of life and highlight the importance of multiple factors, particularly the introduction of solid foods, in shaping the oral microbiome during early life.
Author Contributions
Conceptualization, L.F.S., M.S.P. and D.T.G.; methodology, R.A.A. and L.F.S.; formal analysis, R.A.A. and L.F.S.; investigation, R.A.A. and A.S.C.; resources, D.T.G.; data curation, R.A.A.; writing—original draft preparation, R.A.A.; writing—review and editing, R.A.A., L.F.S., M.S.P. and D.T.G.; visualization, R.A.A.; supervision, L.F.S., C.T.L. and D.T.G.; funding acquisition, D.T.G. All authors have read and agreed to the published version of the manuscript.
Funding
This research was funded by an unrestricted research grant from Medela AG (Switzerland), administered by The University of Western Australia. R.A.A. is supported by a PhD postgraduate scholarship from Saudi Arabia. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.
Institutional Review Board Statement
The study was conducted in accordance with the Declaration of Helsinki and approved by the Human Research Ethics Committee of The University of Western Australia (RA/4/20/4023).
Informed Consent Statement
Informed consent was obtained from all subjects involved in this study.
Data Availability Statement
Sequence data have been submitted to the NCBI SRA (BioProject Submission: SUB14691294).
Acknowledgments
The authors would like to acknowledge Erika van der Dries for collecting the samples.
Conflicts of Interest
D.T.G. declares participation in the Scientific Advisory Board of Medela AG. C.T.L., L.F.S. and D.T.G. are/were supported by an unrestricted research grant from Medela AG, administered by The University of Western Australia. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results. All other authors declare no conflicts of interest.
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