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Methods and Protocols, Volume 7, Issue 2

April 2024 - 18 articles

Cover Story: In immunological research, bone marrow-derived dendritic cells (BMDCs) are a valuable tool in the study of antigen processing and presentation, as well as T-cell responses. However, the isolation of murine bone marrow for large-scale experiments can be time-consuming. In this protocol, we evaluated a faster isolation method that uses centrifugation prior to the differentiation of FMS-like tyrosine kinase 3 ligand-driven BMDCs. Compared to the conventional flush-out method, we found that the new approach resulted in a similar initial bone marrow cell count as well as viability and an increased yield of BMDCs upon eight days of differentiation, while preserving the proportions of individual dendritic cell subsets (cDC1, cDC2 and pDC). View this paper
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Articles (18)

  • Study Protocol
  • Open Access
3 Citations
5,015 Views
10 Pages

Effects of a WhatsApp-Assisted Health Educational Intervention for Cardiac Rehabilitation: A Randomized Controlled Clinical Trial Protocol

  • Adriana Marcela Jacome-Hortua,
  • Zully Rocio Rincon-Rueda,
  • Diana C. Sanchez-Ramirez and
  • Adriana Angarita-Fonseca

Although the effectiveness of cardiac rehabilitation (CR) programs in secondary prevention is well-recognized, there is a lack of studies exploring the potential of mobile health to enhance educational interventions within CR. The objective is to ass...

  • Study Protocol
  • Open Access
3 Citations
2,684 Views
11 Pages

Financial Toxicity in Renal Patients (FINTORE) Study: A Cross-Sectional Italian Study on Financial Burden in Kidney Disease—A Project Protocol

  • Rossella Siligato,
  • Guido Gembillo,
  • Emanuele Di Simone,
  • Alessio Di Maria,
  • Simone Nicoletti,
  • Laura Maria Scichilone,
  • Matteo Capone,
  • Francesca Maria Vinci,
  • Marta Bondanelli and
  • Cristina Malaventura
  • + 5 authors

Financial toxicity (FT) refers to the negative impact of health-care costs on clinical conditions. In general, social determinants of health, especially poverty, socioenvironmental stressors, and psychological factors, are increasingly recognized as...

  • Article
  • Open Access
3 Citations
2,580 Views
20 Pages

The underground mining environment is currently based on technology that uses mainly analogue sensors in machine and equipment control systems. The primary machine performing the most important functions in a mining system is the powered roof support...

  • Communication
  • Open Access
1 Citations
2,356 Views
8 Pages

Optimization of the Amplification of Equine Muscle-Derived Mesenchymal Stromal Cells in a Hollow-Fiber Bioreactor

  • Julien Duysens,
  • Hélène Graide,
  • Ariane Niesten,
  • Ange Mouithys-Mickalad,
  • Justine Ceusters and
  • Didier Serteyn

The main causes of mortality in horses are the gastrointestinal pathologies associated with septic shock. Stem cells have shown, through systemic injection, a capacity to decrease inflammation and to regenerate injured tissue faster. Nevertheless, to...

  • Study Protocol
  • Open Access
2,671 Views
12 Pages

Assessing Stress Induced by Fluid Shifts and Reduced Cerebral Clearance during Robotic-Assisted Laparoscopic Radical Prostatectomy under Trendelenburg Positioning (UroTreND Study)

  • Tobias Peschke,
  • Matthias Feuerecker,
  • Daniel Siegl,
  • Nathalie Schicktanz,
  • Christian Stief,
  • Peter Zu Eulenburg,
  • Alexander Choukér and
  • Judith-Irina Buchheim

In addition to general anesthesia and mechanical ventilation, robotic-assisted laparoscopic radical prostatectomy (RALP) necessitates maintaining a capnoperitoneum and placing the patient in a pronounced downward tilt (Trendelenburg position). While...

  • Protocol
  • Open Access
1,978 Views
11 Pages

Diverting the Use of Hand-Operated Tablet Press Machines to Bioassays: A Novel Protocol to Test ‘Waste’ Insoluble Shell Matrices

  • Camille Lutet-Toti,
  • Marie Da Silva Feliciano,
  • Nelly Debrosse,
  • Jérôme Thomas,
  • Laurent Plasseraud and
  • Frédéric Marin

To mineralize their shells, molluscs secrete a complex cocktail of proteins—collectively defined as the calcifying shell matrix—that remains occluded in the exoskeleton. Nowadays, protein extracts from shells are recognized as a potential...

  • Review
  • Open Access
11 Citations
8,808 Views
14 Pages

Imaging Flow Cytometry: Development, Present Applications, and Future Challenges

  • Savvas Dimitriadis,
  • Lefkothea Dova,
  • Ioannis Kotsianidis,
  • Eleftheria Hatzimichael,
  • Eleni Kapsali and
  • Georgios S. Markopoulos

Imaging flow cytometry (ImFC) represents a significant technological advancement in the field of cytometry, effectively merging the high-throughput capabilities of flow analysis with the detailed imaging characteristics of microscopy. In our comprehe...

  • Article
  • Open Access
2,402 Views
15 Pages

Indirect Measurement of Variables in a Heterogeneous Reaction for Biodiesel Production

  • Ana Paloma González-García,
  • Lourdes Díaz-Jiménez,
  • Padmasree K. Padmadas and
  • Salvador Carlos-Hernández

This research focuses on the development of a state observer for performing indirect measurements of the main variables involved in the soybean oil transesterification reaction with a guishe biochar-based heterogeneous catalyst; the studied reaction...

  • Study Protocol
  • Open Access
2 Citations
4,208 Views
14 Pages

The Frailty Reduction via Implementation of Exercise, Nutrition, and Deprescribing (FRIEND) Trial: Study Protocol and Recruitment Results

  • Michael Inskip,
  • Carolina Almendrales Rangel,
  • Chidiamara Maria Njoku,
  • Fiona Barnett,
  • Isabel Shih,
  • Leonie O’Neill,
  • Maria A. Fiatarone Singh and
  • Trinidad Valenzuela

Introduction: Virtually all adults in aged care facilities are frail, a condition which contributes to falls, cognitive decline, hospitalisation, and mortality. Polypharmacy, malnutrition, sedentariness, and sarcopenia are risk factors amenable to in...

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Methods Protoc. - ISSN 2409-9279