Hyaluronic Acid-Poly(N-acryloyl glycinamide) Copolymers as Sources of Degradable Thermoresponsive Hydrogels for Therapy
Abstract
:1. Introduction
2. Results and Discussion
2.1. Synthesis
2.2. Spectroscopic Analyses
2.3. Indirect Demonstration of the Grafting of PNAGA onto HA Macromolecules
2.3.1. Physical Aspect
2.3.2. Molar Mass Determination
2.3.3. Ionic Interaction with Cationic Poly(l-Lysine)
2.3.4. Degradation by Hyaluronidase
2.4. Ability to Sustaining the Release of a Drug
3. Conclusions
4. Materials and Methods
4.1. Materials
4.1.1. Chemicals
4.1.2. Synthesis of HA-PNAGA Copolymer
4.2. Methods
4.2.1. Spectral Characterizations
4.2.2. Thermal Characterizations
4.2.3. Size Exclusion Chromatography (SEC)
4.2.4. Enzymatic Degradation
4.2.5. Formulation of Prednisolone Hydrogels
4.2.6. Prednisolone Sustained Release Profiles
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Polymers | HA,Na a g | NAGA a g | HA/ HA + NAGA b W/W % | HA/ HA + NAGA c g W/W % (Moldisac%) | Yield % ± 2 | Gel → Sol Transition Temperature d °C |
---|---|---|---|---|---|---|
HA30 − PNAGA 1 | 0.3 | 1.2 | 20 | 97 | 55–56 | |
HA100 − PNAGA 2 | 0.375 | 1.5 | 20 | 23 (7.4) | 96 | 71–72 |
HA100 − PNAGA 3 | 0.065 | 2.0 | 3.15 | 96 | 66–68 | |
HA100 − PNAGA 4 | 0.25 | 2.5 | 9.18 | 9.2 (2.93) | 95 | 83–84 |
HA100 − PNAGA 5 | 0.4 | 2.5 | 13.8 | 17.7 (5.7) | 95 | 85–87 |
HA30 − PNAGA 6 | 0.4 | 0.4 | 50 | 54.4 (17.4) | 97 | 43–45 e |
HA100 − PNAGA 7 | 0.4 | 0.4 | 50 | 98 | 41–42 e | |
HA100 − PNAGA 8 | 0.4 | 0.4 | 50 | 56.4 (18) | 94 | 42–43 e |
HA2000 − PNAGA 9 | 0.4 | 2.5 | 13.8 | 96 | 77–79 | |
HA2000 − PNAGA 10 | 0.2 | 2.5 | 9.18 | 9.2 (2.9) | 95 | 73–75 |
HA30 + PNAGA121 | 0.01 | 0.01 | 50 | 50 (16 ± 1) | - | - |
HA100 + PNAGA121 | 0.01 | 0.01 | 50 | 53 (17 ± 1) | - | 58–59 f |
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Boustta, M.; Vert, M. Hyaluronic Acid-Poly(N-acryloyl glycinamide) Copolymers as Sources of Degradable Thermoresponsive Hydrogels for Therapy. Gels 2020, 6, 42. https://doi.org/10.3390/gels6040042
Boustta M, Vert M. Hyaluronic Acid-Poly(N-acryloyl glycinamide) Copolymers as Sources of Degradable Thermoresponsive Hydrogels for Therapy. Gels. 2020; 6(4):42. https://doi.org/10.3390/gels6040042
Chicago/Turabian StyleBoustta, Mahfoud, and Michel Vert. 2020. "Hyaluronic Acid-Poly(N-acryloyl glycinamide) Copolymers as Sources of Degradable Thermoresponsive Hydrogels for Therapy" Gels 6, no. 4: 42. https://doi.org/10.3390/gels6040042