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Open AccessArticle

Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock

1
ANSES, Laboratoire de la Santé des Végétaux-Unité de Mycologie, Domaine de Pixérécourt-Bât. E, CS 40009, F54220 Malzéville, France
2
Department of Plant and Soil Science, School of Biological Sciences, University of Aberdeen, Aberdeen, Scotland AB24 3UU, UK
3
Wageningen Plant Research, Business Unit Biointeractions & Plant Health, Wageningen UR, 6700 AA Wageningen, The Netherlands
*
Author to whom correspondence should be addressed.
Academic Editor: Thomas Jung
J. Fungi 2021, 7(2), 87; https://doi.org/10.3390/jof7020087
Received: 21 December 2020 / Revised: 21 January 2021 / Accepted: 24 January 2021 / Published: 27 January 2021
Numerous Phytophthora and Pythium disease outbreaks have occurred in Europe following inadvertent introduction of contaminated ornamental plants. Detection and identification of pathogens are crucial to reduce risks and improve plant biosecurity in Europe and globally. Oomycete diversity present in roots and compost was determined in 99 hardy woody plants bought from nurseries, retailers and internet sellers, using both isolations and molecular analyses. Oomycete DNA was quantified using real-time PCR of environmental DNA from the plants using three loci: ITS, trnM-trnP-trnM and atp9-nad9. At least one oomycete species was isolated from 89.9% of plants using classical techniques. In total, 10 Phytophthora spp., 17 Pythium spp. and 5 Phytopythium spp. were isolated. Oomycetes were isolated from 86% of asymptomatic plants, but real-time PCR demonstrated that oomycetes were associated with all plants tested. More oomycete DNA occurred in composts in comparison with roots and filters from baiting water (a mean of 7.91 ng g−1, 6.55 × 10−1 ng g−1 and 5.62 × 10−1 ng g−1 of oomycete DNA detected in compost with ITS, trnM and atp9 probes, respectively); the ITS probe detected the highest quantities of oomycete DNA. No significant differences were found in quantities of oomycete DNA detected using real-time PCR in plants purchased online or from traditional retailers. View Full-Text
Keywords: TaqMan PCR; Phytophthora; Pythium; quantification; ornamental plants; international trade TaqMan PCR; Phytophthora; Pythium; quantification; ornamental plants; international trade
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MDPI and ACS Style

Puertolas, A.; Bonants, P.J.M.; Boa, E.; Woodward, S. Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock. J. Fungi 2021, 7, 87. https://doi.org/10.3390/jof7020087

AMA Style

Puertolas A, Bonants PJM, Boa E, Woodward S. Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock. Journal of Fungi. 2021; 7(2):87. https://doi.org/10.3390/jof7020087

Chicago/Turabian Style

Puertolas, Alexandra; Bonants, Peter J.M.; Boa, Eric; Woodward, Steve. 2021. "Application of Real-Time PCR for the Detection and Quantification of Oomycetes in Ornamental Nursery Stock" J. Fungi 7, no. 2: 87. https://doi.org/10.3390/jof7020087

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