Retrospective Longitudinal Survey on Canine Vector-Borne Pathogens: Trends and Challenges of 10 Years of Activities of a Veterinary Blood Bank
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sampling Population
2.2. Pre-Donor Screening for CVBPs
2.3. Statistical Analysis
2.4. Ethical Statement
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
- Reine, N.J. Infection and Blood Transfusion: A Guide to Donor Screening. Clin. Tech. Small Anim. Pract. 2004, 19, 68–74. [Google Scholar] [CrossRef]
- Spada, E.; Proverbio, D.; Martino, P.A.; Baggiani, L.; Roggero, N. Ammonia Concentration and Bacterial Evaluation of Feline Whole Blood and Packed Red Blood Cell Units Stored for Transfusion. Int. J. Health Anim. Sci. Food Saf. 2014, 1, 15–23. [Google Scholar]
- Marenzoni, M.L.; Antognoni, M.T.; Baldelli, F.; Miglio, A.; Stefanetti, V.; Desario, C.; Di Summa, A.; Buonavoglia, C.; Decaro, N. Detection of Parvovirus and Herpesvirus DNA in the Blood of Feline and Canine Blood Donors. Vet. Microbiol. 2018, 224, 66–69. [Google Scholar] [CrossRef]
- Pennisi, M.G.; Hartmann, K.; Addie, D.D.; Lutz, H.; Gruffydd-Jones, T.; Boucraut-Baralon, C.; Egberink, H.; Frymus, T.; Horzinek, M.C.; Hosie, M.J.; et al. Blood Transfusion in Cats: ABCD Guidelines for Minimising Risks of Infectious Iatrogenic Complications. J. Feline Med. Surg. 2015, 17, 588–593. [Google Scholar] [CrossRef]
- Wardrop, K.J.; Birkenheuer, A.; Blais, M.C.; Callan, M.B.; Kohn, B.; Lappin, M.R.; Sykes, J. Update on Canine and Feline Blood Donor Screening for Blood-Borne Pathogens. J. Vet. Intern. Med. 2016, 30, 15–35. [Google Scholar] [CrossRef] [PubMed]
- Miglio, A.; Stefanetti, V.; Antognoni, M.T.; Cappelli, K.; Capomaccio, S.; Coletti, M.; Passamonti, F. Stored Canine Whole Blood Units: What Is the Real Risk of Bacterial Contamination? J. Vet. Intern. Med. 2016, 30, 1830–1837. [Google Scholar] [CrossRef]
- Stefanetti, V.; Miglio, A.; Cappelli, K.; Capomaccio, S.; Sgariglia, E.; Marenzoni, M.L.; Antognoni, M.T.; Coletti, M.; Mangili, V.; Passamonti, F. Detection of Bacterial Contamination and DNA Quantification in Stored Blood Units in 2 Veterinary Hospital Blood Banks. Vet. Clin. Pathol. 2016, 45, 406–410. [Google Scholar] [CrossRef]
- Marenzoni, M.L.; Lauzi, S.; Miglio, A.; Coletti, M.; Arbia, A.; Paltrinieri, S.; Antognoni, M.T. Comparison of Three Blood Transfusion Guidelines Applied to 31 Feline Donors to Minimise the Risk of Transfusion-Transmissible Infections. J. Feline Med. Surg. 2018, 20, 663–673. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Carli, E.; Carminato, A.; Ravagnan, S.; Capello, K.; Antognoni, M.T.; Miglio, A.; Furlanello, T.; Proverbio, D.; Spada, E.; Stefani, A.; et al. Frequency of DEA 1 Antigen in 1037 Mongrel and purebreed Dogs in ITALY. BMC Vet. Res. 2017, 13, 364. [Google Scholar] [CrossRef] [Green Version]
- Miglio, A.; Gavazza, A.; Siepi, D.; Bagaglia, F.; Misia, A.; Antognoni, M.T. Hematological and Biochemical Reference Intervals for 5 Adult Hunting Dog Breeds Using a Blood Donor Database. Animals 2020, 10, 1212. [Google Scholar] [CrossRef]
- Freeman, M.J.; Kirby, B.M.; Panciera, D.L.; Henik, R.A.; Rosin, E.; Sullivan, L.J. Hypotensive shock syndrome associated with acute Babesia canis infection in a dog. J. Am. Vet. Med. Assoc. 1994, 204, 94–96. [Google Scholar]
- Owens, S.D.; Oakley, D.A.; Marryott, K.; Hatchett, W.; Walton, R.; Nolan, T.J.; Newton, A.; Steurer, F.; Schantz, P.; Giger, U. Transmission of visceral leishmaniasis through blood transfusions from infected English foxhounds to anemic dogs. J. Am. Vet. Med. Assoc. 2001, 219, 1076–1083. [Google Scholar] [CrossRef] [PubMed]
- Stegeman, J.R.; Birkenheuer, A.J.; Kruger, J.M.; Breitschwerdt, E.B. Transfusion-associated Babesia gibsoni infection in a dog. J. Am. Vet. Med. Assoc. 2003, 222, 959–963. [Google Scholar] [CrossRef]
- de Freitas, E.; Melo, M.N.; da Costa-Val, A.P.; Michalick, M.S.M. Transmission of Leishmania Infantum via Blood Transfusion in Dogs: Potential for Infection and Importance of Clinical Factors. Vet. Parasitol. 2006, 137, 159–167. [Google Scholar] [CrossRef]
- Pérez Pérez, P.; Rodríguez-Escolar, I.; Carretón, E.; Sánchez Agudo, J.Á.; Lorenzo-Morales, J.; Montoya-Alonso, J.A.; Morchón, R. Serological Survey of Canine Vector-Borne Infections in North-Center Spain. Front. Vet. Sci. 2021, 8, 784331. [Google Scholar] [CrossRef]
- Gizzarelli, M.; Foglia Manzillo, V.; Ciuca, L.; Morgoglione, M.E.; El Houda Ben Fayala, N.; Cringoli, G.; Oliva, G.; Rinaldi, L.; Maurelli, M.P. Simultaneous Detection of Parasitic Vector Borne Diseases: A Robust Cross-Sectional Survey in Hunting, Stray and Sheep Dogs in a Mediterranean Area. Front. Vet. Sci. 2019, 6, 288. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Sainz, Á.; Roura, X.; Miró, G.; Estrada-Peña, A.; Kohn, B.; Harrus, S.; Solano-Gallego, L. Guideline for Veterinary Practitioners on Canine Ehrlichiosis and Anaplasmosis in Europe. Parasites Vectors 2015, 8, 75. [Google Scholar] [CrossRef] [Green Version]
- Esteban-Mendoza, M.V.; Arcila-Quiceno, V.; Albarracín-Navas, J.; Hernández, I.; Flechas-Alarcón, M.C.; Morchón, R. Current Situation of the Presence of Dirofilaria immitis in Dogs and Humans in Bucaramanga, Colombia. Front. Vet. Sci. 2020, 7, 488. [Google Scholar] [CrossRef]
- Montoya-Alonso, J.A.; Morchón, R.; Costa-Rodríguez, N.; Matos, J.I.; Falcón-Cordón, Y.; Carretón, E. Current Distribution of Selected Vector-Borne Diseases in Dogs in Spain. Front. Vet. Sci. 2020, 7, 564429. [Google Scholar] [CrossRef]
- Mendoza-Roldan, J.A.; Benelli, G.; Bezerra-Santos, M.A.; Nguyen, V.-L.; Conte, G.; Iatta, R.; Furlanello, T.; Otranto, D. Seropositivity to Canine Tick-Borne Pathogens in a Population of Sick Dogs in Italy. Parasites Vectors 2021, 14, 292. [Google Scholar] [CrossRef]
- Solano-Gallego, L.; Miró, G.; Koutinas, A.; Cardoso, L.; Pennisi, M.G.; Ferrer, L.; Bourdeau, P.; Oliva, G.; Baneth, G. LeishVet Guidelines for the Practical Management of Canine Leishmaniosis. Parasites Vectors 2011, 4, 86. [Google Scholar] [CrossRef] [Green Version]
- Ministero della Salute. Linea Guida Relativa All’esercizio Delle Attività Riguardanti La Medicina Trasfusionale in Campo Veterinario. Available online: http://95.110.157.84/gazzettaufficiale.biz/atti/2016/20160025/16A00611.htm (accessed on 1 March 2022).
- Di Muccio, T.; Veronesi, F.; Antognoni, M.T.; Onofri, A.; Piergili Fioretti, D.; Gramiccia, M. Diagnostic Value of Conjunctival Swab Sampling Associated with Nested PCR for Different Categories of Dogs Naturally Exposed to Leishmania infantum Infection. J. Clin. Microbiol. 2012, 50, 2651–2659. [Google Scholar] [CrossRef] [Green Version]
- Morganti, G.; Gavaudan, S.; Canonico, C.; Ravagnan, S.; Olivieri, E.; Diaferia, M.; Marenzoni, M.L.; Antognoni, M.T.; Capelli, G.; Silaghi, C.; et al. Molecular Survey on Rickettsia spp., Anaplasma phagocytophilum, Borrelia burgdorferi sensu lato, and Babesia spp. in Ixodes ricinus Ticks Infesting Dogs in Central Italy. Vector Borne Zoonotic Dis. 2017, 17, 743–748. [Google Scholar] [CrossRef]
- Stefanetti, V.; Morganti, G.; Veronesi, F.; Gavaudan, S.; Capelli, G.; Ravagnan, S.; Antognoni, M.T.; Bianchi, F.; Passamonti, F. Exposure of Owned Dogs and Feeding Ticks to Spotted Fever Group Rickettsioses in Central Italy. Vector Borne Zoonotic Dis. 2018, 18, 704–708. [Google Scholar] [CrossRef]
- Gradoni, L.; Gramiccia, M. OIE Manual of Diagnostic Tests and Vaccines for Terrestrial Animals (Mammals, Birds and Bees). In Leishmaniosis; Office International des Epozooties: Paris, France, 2008; pp. 240–250. [Google Scholar]
- Adel, A.; Berkvens, D.; Abatih, E.; Soukehal, A.; Bianchini, J.; Saegerman, C. Evaluation of Immunofluorescence Antibody Test Used for the Diagnosis of Canine Leishmaniasis in the Mediterranean Basin: A Systematic Review and Meta-Analysis. PLoS ONE 2016, 11, e0161051. [Google Scholar] [CrossRef]
- Euzeby, J. Diagnostic Expérimental Des Helminthoses Animales: Animaux Domestiques, Animaux de Laboratoire Primates Travaux Pratiques d’hel-Minthologie Vétérinaire. In Livre 1: Généralites, Diagnostic Ante Mortem; Editeur Ministère de l’Agriculture: Paris, France, 1981; p. 349. [Google Scholar]
- Antognoni, M.T.; Vascellari, M.; Da Rold, G.; Toniolo, F.; Sgubin, S.; Zanardello, C.; Carminato, A.; Miglio, A. Looking for Dog Blood Donors in an Endemic Area for Vector-Borne Infections of Central Italy. Animals 2022, 12, 817. [Google Scholar] [CrossRef]
- Maresca, C.; Scoccia, E.; Barizzone, F.; Catalano, A.; Mancini, S.; Pagliacci, T.; Porrini, M.; Principato, M.; Venditti, G.; Grelloni, V. A Survey on Canine Leishmaniasis and Phlebotomine Sand Flies in Central Italy. Res. Vet. Sci. 2009, 87, 36–38. [Google Scholar] [CrossRef]
- Ebani, V.V. Serological Survey of Ehrlichia canis and Anaplasma phagocytophilum in Dogs from Central Italy: An Update (2013–2017). Pathogens 2019, 8, 3. [Google Scholar] [CrossRef] [Green Version]
- Mylonakis, M.E.; Harrus, S.; Breitschwerdt, E.B. An Update on the Treatment of Canine Monocytic Ehrlichiosis (Ehrlichia canis). Vet. J. 2019, 246, 45–53. [Google Scholar] [CrossRef]
- Mendoza-Roldan, J.; Benelli, G.; Panarese, R.; Iatta, R.; Furlanello, T.; Beugnet, F.; Zatelli, A.; Otranto, D. Leishmania infantum and Dirofilaria immitis Infections in Italy, 2009–2019: Changing Distribution Patterns. Parasites Vectors 2020, 13, 193. [Google Scholar] [CrossRef] [Green Version]
- Colombo, M.; Morelli, S.; Simonato, G.; Di Cesare, A.; Veronesi, F.; Frangipane di Regalbono, A.; Grassi, L.; Russi, I.; Tiscar, P.G.; Morganti, G.; et al. Exposure to Major Vector-Borne Diseases in Dogs Subjected to Different Preventative Regimens in Endemic Areas of Italy. Pathogens 2021, 10, 507. [Google Scholar] [CrossRef]
- Ebani, V.V.; Bertelloni, F.; Torracca, B.; Cerri, D. Serological Survey of Borrelia burgdorferi sensu lato, Anaplasma phagocytophilum, and Ehrlichia canis Infections in Rural and Urban Dogs in Central Italy. Ann. Agric. Environ. Med. 2014, 21, 671–675. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Morelli, S.; Gori, F.; Colombo, M.; Traversa, D.; Sarrocco, G.; Simonato, G.; Nespeca, C.; Di Cesare, A.; Frangipane di Regalbono, A.; Veronesi, F.; et al. Simultaneous Exposure to Angiostrongylus vasorum and Vector-Borne Pathogens in Dogs from Italy. Pathogens 2021, 10, 1200. [Google Scholar] [CrossRef] [PubMed]
- Vascellari, M.; Ravagnan, S.; Carminato, A.; Cazzin, S.; Carli, E.; Da Rold, G.; Lucchese, L.; Natale, A.; Otranto, D.; Capelli, G. Exposure to Vector-Borne Pathogens in Candidate Blood Donor and Free-Roaming Dogs of Northeast Italy. Parasites Vectors 2016, 9, 369. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Pascucci, I.; Antognini, E.; Canonico, C.; Montalbano, M.G.; Necci, A.; di Donato, A.; Moriconi, M.; Morandi, B.; Morganti, G.; Crotti, S.; et al. One Health Approach to Rickettsiosis: A Five-Year Study on Spotted Fever Group Rickettsiae in Ticks Collected from Humans, Animals and Environment. Microorganisms 2021, 10, 35. [Google Scholar] [CrossRef]
- Brianti, E.; Arfuso, F.; Cringoli, G.; Di Cesare, A.; Falsone, L.; Ferroglio, E.; Frangipane di Regalbono, A.; Gaglio, G.; Galuppi, R.; Genchi, M.; et al. Italian Nationwide Survey on Endoparasites of Dogs. In Proceedings of the XXX Congresso SoIPa, Milano, Italy, 26–29 June 2018; pp. 45–48. [Google Scholar]
- Harizanov, R.N.; Jordanova, D.P.; Bikov, I.S. Some Aspects of the Epidemiology, Clinical Manifestations, and Diagnosis of Human Dirofilariasis Caused by Dirofilaria repens. Parasitol. Res. 2014, 113, 1571–1579. [Google Scholar] [CrossRef] [PubMed]
- Maia, C.; Coimbra, M.; Ramos, C.; Cristóvão, J.M.; Cardoso, L.; Campino, L. Serological Investigation of Leishmania infantum, Dirofilaria immitis and Angiostrongylus vasorum in Dogs from Southern Portugal. Parasites Vectors 2015, 8, 152. [Google Scholar] [CrossRef] [Green Version]
- Sauda, F.; Malandrucco, L.; Macrì, G.; Scarpulla, M.; De Liberato, C.; Terracciano, G.; Fichi, G.; Berrilli, F.; Perrucci, S. Leishmania infantum, Dirofilaria spp. and Other Endoparasite Infections in Kennel Dogs in Central Italy. Parasite 2018, 25, 2. [Google Scholar] [CrossRef] [Green Version]
- Capelli, G.; Genchi, C.; Baneth, G.; Bourdeau, P.; Brianti, E.; Cardoso, L.; Danesi, P.; Fuehrer, H.-P.; Giannelli, A.; Ionică, A.M.; et al. Recent Advances on Dirofilaria repens in Dogs and Humans in Europe. Parasites Vectors 2018, 11, 663. [Google Scholar] [CrossRef] [PubMed] [Green Version]
End Point Titres (IgG) | ||||||
---|---|---|---|---|---|---|
CVBPs | Diagnostic Methods | Number of Dogs Positive; % (95% CI) | 1/80 (n Dogs) | 1/160 (n Dogs) | 1/320 (n Dogs) | 1/640 (n Dogs) |
Leishmania infantum | IFAT | 154 12.22% (10.41–14.03%) | 95 | 25 | 23 | 11 |
Rickettsia conorii | IFAT | 108 20.15% * (16.72–23.50%) | 102 | 5 | 1 | 0 |
Anaplasma phagocytophilum | IFAT | 15 1.19% (0.59–1.79%) | 7 | 1 | 4 | 2 |
1/25 (n Dogs) | 1/50 (n Dogs) | 1/100 (n Dogs) | 1/200 (n Dogs) | |||
Ehrlichia canis | IFAT | 29 2.30% (1.47–3.13%) | 17 | 5 | 5 | 2 |
1/64 (n Dogs) | 1/128 (n Dogs) | 1/256 (n Dogs) | 1/512 (n Dogs) | |||
Babesia canis | IFAT | 2 0.16% (0.00–0.38%) | 2 | 0 | 0 | 0 |
Dirofilaria repens | Knott test | 12 0.95% (0.42–1.49%) | - | - | - | - |
Dirofilaria immitis | Knott test/ELISA | 4 0.32% (0.01–0.63%) | - | - | - | - |
Year 2012 | Year 2013 | Year 2014 | Year 2015 | Year 2016 | Year 2017 | Year 2018 | Year 2019 | Year 2020 | Year 2021 | |
---|---|---|---|---|---|---|---|---|---|---|
N. of dogs | 118 | 208 | 211 | 152 | 143 | 119 | 91 | 88 | 77 | 55 |
Leishmania infantum n, % (95% CI) | 14 11.86% (6.03–17.70%) | 36 17.30% (12.17–22.45%) | 32 15.17% (10.33–20.01%) | 23 15.13% (9.43–20.83%) | 19 13.28% (7.72–18.85%) | 12 10.08% (4.67–15.49%) | 8 8.79% (2.97–14.61%) | 4 4.55% (0.19–8.90%) | 4 5.20% (0.24–10.15%) | 2 3.64% (0.00–8.58%) |
Ehrlichia canis n, % (95% CI) | 4 3.38% (0.12–6.66%) | 3 1.44% (0.00–3.06%) | 5 2.38% (0.32–4.42%) | 7 4.60% (1,27–7.94%) | 0 0.00% (0.00–0.00%) | 9 8.00% (2.81–13.19%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 1 1.82% (0.00–5.35%) |
Anaplasma phagocytophilum n, % (95% CI) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 4 2.80% (0.09–5.50%) | 4 3.60% (0.60–6.60%) | 1 1.00% (0.00–3.24%) | 3 3.44% (0.00–7.20%) | 2 2.60% (0.00–6.15%) | 1 1.82% (0.00–5.35%) |
Babesia canis n, % (95% CI) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 1 0.60% (0.00–1.94%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 1 1.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) |
Dirofilaria immitis n,% (95% CI) | 1 0.84% (0.00–2.50%) | 1 0.48% (0.00–1.42%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 1 0.70% (0.00–2.07%) | 1 0.90% (0.00–2.48%) | 0 0.00% (0.00–0.00%) | 0 0,00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) |
Dirofilaria repens n,% (95% CI) | 0 0.00% (0.00–0.00%) | 2 1.00% (0.00–2.29%) | 4 1.90% (0.06–3.74%) | 0 0.00% (0.00–0.00%) | 0 0.00% (0.00–0.00%) | 2 1.70% (0.00–3.99%) | 2 2.20% (0.00–5.21%) | 1 1,14% (0.00–3.35%) | 1 1.30% (0.00–3.83%) | 0 0.00% (0.00–0.00%) |
Rickettsia conorii n,% (95% CI) | 24 20.33% (13.08–27.60%) | 47 22.60% (16.91–38.38%) | 37 17.54% (12.40–22.67%) | n.p. | n.p. | n.p. | n.p. | n.p. | n.p. | n.p. |
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Morganti, G.; Miglio, A.; Moretta, I.; Misia, A.L.; Rigamonti, G.; Cremonini, V.; Antognoni, M.T.; Veronesi, F. Retrospective Longitudinal Survey on Canine Vector-Borne Pathogens: Trends and Challenges of 10 Years of Activities of a Veterinary Blood Bank. Vet. Sci. 2022, 9, 274. https://doi.org/10.3390/vetsci9060274
Morganti G, Miglio A, Moretta I, Misia AL, Rigamonti G, Cremonini V, Antognoni MT, Veronesi F. Retrospective Longitudinal Survey on Canine Vector-Borne Pathogens: Trends and Challenges of 10 Years of Activities of a Veterinary Blood Bank. Veterinary Sciences. 2022; 9(6):274. https://doi.org/10.3390/vetsci9060274
Chicago/Turabian StyleMorganti, Giulia, Arianna Miglio, Iolanda Moretta, Ambra L. Misia, Giulia Rigamonti, Valentina Cremonini, Maria T. Antognoni, and Fabrizia Veronesi. 2022. "Retrospective Longitudinal Survey on Canine Vector-Borne Pathogens: Trends and Challenges of 10 Years of Activities of a Veterinary Blood Bank" Veterinary Sciences 9, no. 6: 274. https://doi.org/10.3390/vetsci9060274