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Beverages 2019, 5(1), 8; https://doi.org/10.3390/beverages5010008

Pulsed Electric Field Extraction and Antioxidant Activity Determination of Moringa oleifera Dry Leaves: A Comparative Study with Other Extraction Techniques

1
Department of Food Technology, Technological Educational Institute of Thessaly, Terma N. Temponera str., GR-43100 Karditsa, Greece
2
Department of Wine, Vine and Beverage Sciences, University of West Attica, Agiou Spyridonos str., GR-12210 Athens, Greece
*
Author to whom correspondence should be addressed.
Received: 7 December 2018 / Revised: 24 December 2018 / Accepted: 9 January 2019 / Published: 13 January 2019
(This article belongs to the Special Issue Pulse Electric Field in Liquid Food Processing and Extraction)
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Abstract

The scope of this work was to determine the possibility of the application of the pulsed electric field (PEF) technique to the production of extracts from Moringa oleifera plant material (freeze-dried leaves). Various PEF conditions (pulse duration—PD; and pulse interval—PI) were tested. A field strength of 7 kV/cm was used. The total phenols in the extracts were evaluated by the Folin–Ciocalteu method and the antioxidant activity was evaluated by the radical scavenging activity (DPPH), ferric reducing antioxidant power (FRAP) and Rancimat methods. The results were compared with those of the extracts obtained using other extraction techniques, namely microwave-assisted and ultrasound-assisted extractions, simple boiling water extraction, and plain maceration with water (as the control). The highest extraction of total phenols was achieved by the PEF procedure using 40 min treatment at a PD of 20 msec and a PI of 100 μsec. Additionally, all methods for the determination of the antioxidant activity showed that the activity of the extracts was proportional to the total phenol content. Concerning the PEF procedure, a low pulse duration with a high pulse interval is proposed in order to achieve higher extraction efficiency. View Full-Text
Keywords: pulsed electric field; Moringa oleifera; freeze-dried leaves; extraction; antioxidant activity pulsed electric field; Moringa oleifera; freeze-dried leaves; extraction; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited (CC BY 4.0).
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Bozinou, E.; Karageorgou, I.; Batra, G.; G. Dourtoglou, V.; I. Lalas, S. Pulsed Electric Field Extraction and Antioxidant Activity Determination of Moringa oleifera Dry Leaves: A Comparative Study with Other Extraction Techniques. Beverages 2019, 5, 8.

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